Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"multiplex qPCR"”.
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Investigation and validation of mammalian telomeres as biomarkers for health and disease
… oxidative damage. A mouse adapted monochromatic multiplex qPCR (mmqPCR) assay was developed and validated to measure relative TL, complemented by a formamidopyrimidine DNA glycosylase (FPG) qPCR assay to quantify telomere-specific oxidative damage. Across multiple murine tissues, pronounced …
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Characterizing the epidemiology of ophidiomycosis in North American snakes through field studies, modeling, pathogen genomic analysis, and treatment trials
… to detect the presence of O. ophidiicola using qPCR. Each snake was assigned to an ophidiomycosis category based on the presence of skin lesions and O. ophidiicola. Logistic regression models were used to predict lesion presence, O. ophidiicola detection, and ophidiomycosis category based on …
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Accelerated Sepsis Diagnosis by Seamless Integration of Nucleic Acid Purification and Detection
… diagnostic information. Although the present qPCR-based test is more specific than biomarker detection and faster than culturing, its 6 ~ 10 hr turnaround remains suboptimal relative to the 7.6%/hr rapid deterioration of the survival rate, and the 3 hr hands-on time is labor-intensive. To …
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Nucleic acid sample preparation using magnetic ionic liquids as cell lysis and DNA extraction solvents
… and directly integrated into quantitative PCR (qPCR) and multiplex-qPCR assays with SYBR green and Taqman probe detection. Adding the DNA-enriched MIL into the reaction buffer drastically reduced the sample preparation time improving sample throughputs without inhibiting PCR efficiency. A …
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Messenger Rna Profiling: A Prototype Method For Body Fluidand Tissue Identification
… fluid-specific genes have been incorporated into multiplex PCR and real-time PCR assays. These assays allow for the positive identification of blood, saliva, semen,vaginal secretions, and/or menstrual blood in a stain. The final task of this work was the molecular characterization of mRNA …