Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 18 of 18 for “"multiplex assay"”.
-
MULTIPLEX ASSAY DESIGN
This thesis explores the design of assays for the multiplexed detection of various infectious diseases. A single-pot, 8-plex end-point PCR reaction for the detection of carbapenemase-producing Enterobacteriaceae (CPE), vancomycin-resistant Enterococcus (VRE) and colistin resistance has been …
-
Development and Testing of a Rapid Multiplex Assay for the Identification of Biological Stains
… Current antibody- and enzyme activity-based assays used by forensic practitioners for biological stain identification yield only presumptive results. Positive results with non-target body fluids or cross-reactivity with non-human sources has also been well documented. Some tests can consume …
-
Development, Validation and Applications of a Novel Multiplex Assay RM-Yplex Amplifying 13 Rapidly Mutating Y Chromosome Short Tandem Repeat Regions
A polymerase chain reaction (PCR) multiplex assay capable of amplifying 13 rapidly mutating Y chromosome short tandem repeats (RM Y-STRs) simultaneously was developed and optimised. This multiplex assay which was termed RM-Yplex is the first to include all 13 RM Y-STRs including DYF387S1, DYF399S1, …
-
Determination of the frequency of four pathogenic variants causing inborn errors of metabolism in the western cape black population, using a multiplexed arms pcr approach
… a simple, cost effective, and robust method like multiplexed ARMS PCR using standard PCR chemistry is attractive for use in resource constrained environments in which common population variants account for most of the disease burden. Methods: ARMS PCR primers were designed to detect the four …
-
An evaluation of genetic markers for forensic identification of human body fluids
… require large amount of template, use separate assays for various body fluids, and are prone to contamination. Various genes are expressed in different body fluids that could be used as genetic markers for body fluid identification, and are used in forensic investigations. The aim of this study …
-
The Role of the CD14 molecule in equine endotoxemia
… LPS. Supernatants were collected at 6 hours and assayed for tumor necrosis factor ° (TNF°) activity. Part 2; Serum sCD14 was measured at admission and then at 24 and 48 hours after admission using a bead-based multiplex assay. Results - Part 1; Pre-incubation with CD14 mAb did not inhibit …
-
Early biomarkers to predict grade of encephalopathy following hypoxic ischaemic injury
… included; multiple inflammatory proteins using multiplex assay; the metabolomics profile using LC/MS; and the miRNA profile using microarray. Results: Eighty five infants with perinatal depression were recruited. Analysis of inflammatory proteins consisted of exploratory analysis of 37 analytes …
-
Comparison of immune responses induced by Bacillus calmette-Guerin when given at birth or at 6 weeks of age in Ugandan Infants
… blood intracellular cytokine/cytotoxic marker assay to measure T cell-associated cytokine expression and memory phenotypes. We also compared the capacity of BCGspecific T cells to proliferate and produce cytokines upon antigenic stimulation with a 6-day proliferation assay. Finally, we measured …
-
Synthesis and Applications of Luminescent Quantum Dots in Bioassays
… for optical imaging of cells, tissues and in bioassays as alternatives to conventional organic fluorophores. The main objective of my PhD dissertation was to develop luminescent quantum dot based bioassays for real time monitoring of enzyme activity and simultaneous detection of several …
-
Engineering soluble, high affinity T cell receptor domains for detection of staphylococcal and streptococcal exotoxins
… with SEA and also to develop specific, sensitive assays for detection of SEA. In chapter 2, the process of engineering of human Vβ22 protein for improving its affinity towards SEA is described. In this chapter, yeast display coupled with random mutagenesis and fluorescence-activated cell sorting …
-
Contusion Progression in Traumatic Brain Injury
… peri-contusional inflammatory response with a multiplex assay of 42 cytokines, chemokines, and growth factors. Peri- contusional tissue was found to exhibit an early pro-inflammatory signature. • Study IV: A paired microdialysis study of pericontusional expression of matrix metalloproteinases …
-
Elucidating the Immunogenicity and Protective Immune Response of a Trivalent Vaccine for Sudan Ebola, Marburg, and Lassa Fever Viruses
… To do so, I first developed a highly sensitive, multiplex assay for the simultaneous detection of antigen-specific antibodies against several targets of interest, optimising the use of scarce samples from small animal models. I then assessed the immunogenicity of the trivalent and monovalent …
-
Intratracheal instillation of silver nanoparticles exacerbates cardiac ischemia/reperfusion injury in male sprague-dawley rats
… instillation. Serum samples were analyzed by multiplex assay for concentrations of: G-CSF, GM-CSF, MIP-1a, IL -1b, IL-2, IL-5, IL-6, IL-10, IL-13, IL-17a, IL-18, MCP-1, IFNy, RANTES, and TNFa. Twenty four and 168 hours after IT exposure, cardiac ischemia was induced by left anterior descending …
-
Molecular Pathology: Potential Biomarkers For The Detection Of Down Syndrome Pregnancies
… new target biomarkers for DS. We will also use multiplex droplet digital PCR (ddPCR) using cell-free fetal DNA (cffDNA) to target different genes on chromosomes 21, 18, and 13 to screen for trisomy 21 (T21), trisomy 18 (T18) and trisomy 13 (T13), respectively. PRSS56 and SPINK7 proteins are …
-
Investigating the immune modulatory properties of kisspeptin: implications for pregnancy
… Mycobacterium tuberculosis (Mtb) infection assay model as well as a newly established in vitro infection model using lipopolysaccharide (LPS)-stimulated whole blood. Protein expression analysis of selected pro- and anti-inflammatory cytokines was performed on PBMC infected with Mtb and on …
-
Entwicklung einer parallelen Mehrkomponentenanalyse von Antigen-Antikörper-Reaktionen in der Dopinganalyse
… ging. Neben der Verwendung eines Multiplex Ansatzes und der Mikroarraytechnologie stellten ebenfalls die Genauigkeit aller Messparameter, die Stabilität des Versuchsaufbaus sowie die Performance über einen Einfach-Blind-Ansatz Herausforderungen dar. Die Anforderung an den Multiplex …
-
Polyunsaturated fatty acids and inflammatory biomarkers in the pathogenesis of type II diabetes
… IFN-γ, TGF-β and MCP-1) were measured by a multiplex assay that utilises Luminex software technology and vitamin D by chemiluminescence microparticle immunoassay methodology. The FA profiles are reported as mean % weight ± SD and the data obtained compared between DM2 and healthy controls in …
-
Failure of Primary Total Knee Arthroplasty: Establishing a Baseline for Retrieval Analysis Using Well-Functioning Necropsy Specimens
… condyle was assessed for damage. A bead-based multiplex assay using the Luminex MAGPIX platform (R&D Systems, Minneapolis, MN) was performed on the synovial aspirates for simultaneous detection of various inflammatory cytokines including: IL-1β, IL-6, MCP-1, and MIP-3α. Inductively coupled …