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Showing 1 to 20 of 27 for “"multiple reaction monitoring"”.
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Analysis of urinary lipid biomarker candidates from tuberculosis patients by multiple reaction monitoring
… discovery of biomarker candidates, and targeted multiple reaction monitoring (MRM) is the method of choice for subsequent validation of biomarker candidates. A list of candidate urinary biomarkers has previously been generated which can discriminate between latent and active TB infection using …
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LC-MS/MS determination of various drugs of abuse and metabolites in municipal wastewater effluent samples
… ionization was used along with dynamic multiple reaction monitoring (MRM) tandem mass spectrometry for identification and quantitation. The extraction method was validated with matrix matched spiked samples at the limits of quantitation (0.5 ng/mL to 30 ng/mL) for each analyte with …
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Comprehensive proteomic profiling of clinically relevant strains of Mycobacterium tuberculosis
… candidate proteins to use in the design of multiple reaction monitoring (MRM) MS experiments for targeted biomarker validation in patient derived biological samples such as sputum. Liquid chromatography mass spectrometry (LC MS/MS) and data capture were carried out using the LTQ Orbitrap …
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Quantitative Approaches for Protein Differential Expression Analysis
… validation by targeted screening, i.e., multiple reaction monitoring (MRM). Preliminary experiments were performed to evaluate the performance of the spectral counting method. The method proved to be applicable for proteins with spectral counts≥2, and a close-to-linear relationship …
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Liquid Chromatography – Mass Spectrometry for Protein Biomarker Discovery in Pancreatic Adenocarcinoma
… to enhance the utility of the method. Multiple reaction monitoring (MRM)/MS provided sensitivity and specificity to the method and stable isotope dilution (SID) provided correction for losses during sample analysis, enabling precise quantitation and efficient determination of the …
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Computational Tools to Expand the Coverage of Metabolite Profiling via LC-MS/MS
… well as a foundation for designing large scale multiple reaction monitoring experiments.</p><p>With these two additions to the arsenal of LC-MS/MS based metabolite profiling tools, we move closer to one day reaching the goal of global metabolite profiling. In turn, this may lead to exciting …
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Oncoproteomic applications for detection of breast cancer. Proteomic profiling of breast cancer models and biopsies
… of variation for triplicate analyses by multiple reaction monitoring mass spectrometry (MRM-MS), was 9% in cell lines, 17 % in tissue biopsies, 22% in serum samples and 24% in NAF samples. Overall, the results provide a strong paradigm to develop a clinical assay based on proteomic …
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Forensic Analysis of Trace Evidence Fibers and Drugs of Abuse In Biological Matrices Using Chromatographic and Mass Spectrometric Methods
… on dye formulations. Foremost, LC/MS/MS used multiple reaction monitoring (MRM) transitions unique for every dye molecule allowing unequivocal discrimination between fibers as short as 0.5 mm in length and limits of detection for this method range between 200 and 1500 pg for most dyes. CE/DAD …
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Development of liquid chromatography-mass spectrometry protocols for quantification of imidacloprid in pig urine and for analysis of 5α-cyprinol sulfate metabolism in zebrafish
… we developed also included tandem MS/MS and multiple reaction monitoring (MRM) mode that created specific channels to monitor previously identified daughter ions of IMI and the internal standard D4-IMI. The subsequent LC/MS utilization first required purification of 5α-cyprinol sulfate, the …
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Complementary and alternate medicines: a forensic analysis of the potential adulteration of over-the-counter anorectics and "lifestyle" medicines in South Africa
… pharmaceutical ingredients using a targeted multiple reaction monitoring mode. Samples were extracted with 50% v/v methanol in water. A method for the quantitation of sildenafil was subsequently partially validated. The intra- and inter-assay precisions were evaluated and the linearity of the …
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Characterization of membrane lipids and, Changes therein during desiccation and rehydration of the resurrection plant Xerophyta humilis (Bak) Dur and Schinz
… acyl chain compositions were determined by multiple reaction monitoring (MRM) mass spectrometry. The glycerophospholipids profiles from total leaves and roots lipid extracts showed an increased representation of unsaturated molecular species such as 18:3/18:3 (in leaves) and 18:3/18:2 (in …
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Microfluidic Approaches for Probing Protein Phosphorylation in Cells
… also amenable to automation, integration, and multiplexing. To achieve our objective, this study was conducted in multiple steps: (1) We performed a comprehensive analysis of the factors that affect the performance of mass spectrometry detection (i.e., sensitivity, reproducibility, ability to …
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Subcellular Fractionation Methods for the Quantification of Endogenous Protein in Human Liver Using Targeted Proteomics
Ultra-performance Liquid chromatography-multiple reaction monitoring (UPLC-MRM) is a versatile mass spectrometry method with many applications in drug development. Due to its high selectivity, fast analysis time and high sensitivity, UPLC-MRM is widely used in sample bioanalysis for …
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The determination of β-endosulfan and endosulfan sulfate in human serum with dialkylphosphate metabolites as urinary markers using LC-MS/MS electrospray ionization
… mass detection of all the analytes, employing multiple reaction monitoring as scan mode. Calibration standards and quality control samples for both analyses were prepared in the biological matrix in which the samples for each determination were collected, i.e. serum for the determination of the …
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Characterization and quantification of urinary metabolic biomarkers for early response to anti-tuberculosis treatment
… research describes the development of a targeted multiple reaction monitoring (MRM) assay which enabled accurate and precise quantitation of compounds previously detected in an untargeted metabolomics experiment. This targeted assay will be used for validation of these compounds in a larger set of …
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Determination of Rifapentine and 25-O-desacetyl Rifapentine from 100 µl human breastmilk by LC-MS/MS using protein precipitation and solid phase extraction
… 4000 mass spectrometer at unit resolution in the multiple reaction monitoring (MRM) mode was used to monitor the transition of the protonated precursor ions m/z 877.5, m/z 835.4 and m/z 827.4 to the product ions m/z 151.1, m/z 453.2 and m/z 151.200 for rifapentine, 25-Odesacetyl rifapentine and …
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HORMONAL AND GENETIC BASIS OF HEAT RESPONSE IN FIELD PEA (PISUM SATIVUM L.)
… ABA catabolic pathways by liquid chromatography-multiple reaction monitoring mass spectrometry. Both pea HSP genes and ABA metabolism responded rapidly after 3 h at HS. However, PsHSP18.1 and PsHSP71.2 had similar induction levels between heat tolerant and susceptible varieties, suggesting the …
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Development of liquid chromatography with tandem mass spectrometry method for measurement of sepsis biomarkers
… measurement of biomarkers in serum and offers multiplexing capability. Measurements of biomarkers aid clinicians in their decision making and helps them to assess the efficacy of treatment. Hence, leading to improvement in the patient’s health and quality of life. Objective: The aim of this …
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Transfer and Distribution of Cantharidin within Selected Members of Blister Beetles (Coleoptera: Meloidae) and Its Probable Importance in Sexual Behaviour
… MS seemed to be very difficult. Therefore, MRM (Multiple Reaction Monitoring) method for cantharidin/D2C separation and independent quantitation was developed for the first time. Cantharidin pharmacodynamic in male of studied species could be quite different over a period of time. As the …
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