Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 4 of 4 for “"multiple displacement amplification"”.
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Diversity of Skin Infections
… or other microorganisms, using a combination of multiple displacement amplification (MDA), which amplifies all DNA in a sample without any requirement of prior knowledge of the nucleotide sequence, degenerate “general HPV primers” PCR and high-throughput sequencing. The most common microbial DNA …
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Developing and Optimizing Conditions for Single Cell Genetic Analysis
… A recent multi-center study determined a 17% amplification failure rate overall and a 3.4% misdiagnosis rate (ESHRE PGD consortium steering committee, 2002). Experiments presented here were conducted to decrease those rates by optimizing single cell sensitivity and specificity of PGD …
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Investigating Metastatic Lineage In Colorectal Cancer By Single Cell Dna Sequencing
… address this problem, we have developed a highly-multiplexed single cell DNA sequencing method that combines flow-sorting of single nuclei, multiple-displacement-amplification using Φ29 polymerase, low-input library preparation, library barcoding, targeted capture and NGS to generate high-coverage …
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Elucidating aquatic virioplankton diversity and dynamics using high-throughput DNA sequencing
… preparation strategies employ some form of amplification to obtain sufficient DNA for sequencing. Biases resulting from these techniques may alter interpretations of the occurrence and abundance of viral populations from metagenome data. In turn, these biases may lead to misinterpretations …