Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"multiphoton imaging"”.
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Compact Femtosecond Lasers for Multiphoton Imaging
Multiphoton imaging currently is one of the main development directions in Laser Scanning Microscopy. A simplified way to assess suitability of a given laser for MPI applications is a figure of merit (FOM) approach based on peak power and average power of the particular excitation laser used. The …
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Functional Biological Spectroscopy and Multiphoton Imaging Using Endogenous Optical Contrast
… tissue, two model systems were investigated with multiphoton microscopy in order to evaluate processes important in angiogenesis and ovarian carcinogenesis research. Starting with an in vitro model of angiogenesis, the individual intracellular and extracellular optical signals were combined for …
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Label-free multi-modal multiphoton imaging for real-time point-of-procedure assessment of needle biopsies
… demand. Label-free multimodal nonlinear optical imaging (NLOI) provides a powerful and non-invasive tool to study the microstructure and biochemical distributions in biological tissue, especially in tumor microenvironments. Continuous efforts have been devoted to the development and optimization …
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Multiphoton imaging and control of opsins in living neural systems by spectral-temporal modulation of a supercontinuum source
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-08-01
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Supramolecular quantum dot-porphyrin assemblies for biological oxygen sensing
… to enable sensing under two-photon excitation. Multiphoton imaging is a powerful technique that is nondestructive to tissue and provides high-resolution images of live tissue at depths of several hundred microns with submicron spatial resolution. Having studied the photohysical properties of …
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Nonlinear optical frequency conversion of mode-locked all-solid-state lasers
… This novel source is intended for application to multiphoton imaging. The first demonstration of additive pulse mode-locking of a diode-pumped Nd³⁺: YVO₄ laser operating in the near-infrared is presented and the source characteristics compared to a similar system that is mode-locked using a …
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In Vivo Metabolic and Vascular Response to Hypoxia in Twist Knockdown Murine Breast Cancer
… to intrinsic or extrinsic factors hyperspectral imaging was used to determine vascular oxygenation within the tumor. Multiphoton microscopy was used to quantify metabolism on the cellular level by measuring the optical redox ratio as well as the relative contribution ratio of free and bound NADH. …
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Improving the delivery and efficacy of a cancer therapeutic via extracellular matrix modification
… enhance its distribution significantly. In vivo multiphoton imaging of fibrillar collagen type I and injected HSV vectors largely supported the model predictions. Injected viral particles could not penetrate dense networks of fibrillar collagen. Both the initial distribution and subsequent …
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Methods and technologies for high-throughput and high-content small animal screening
… their immobilization for high-resolution imaging and manipulation. Here we present methods to enable HTS and HCS of the nematode Caenorhabditis elegans (C. elegans). First we present microfluidic technologies to rapidly isolate, immobilize, image and manipulate individual animals. These …
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Interstitial Fluid Flow Magnitude and Its Effects on Glioblastoma Invasion
… fluid flow within the brain through simultaneous multiphoton imaging. We showed that this technique can be used to modulate interstitial fluid flow, as a mechanism by which to label cells of interest, and as a means to implant and monitor glioma progression. Through these means we further …
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Multiphoton microscopy for stain-free slide-free histopathology
… advantages, conventional methods for label-free multiphoton imaging are burdened by limited contrast and efficiency of individual modalities as well as complex laser systems, which hinders this technology from wider application in biomedicine. This thesis developed a fiber-based single-excitation …
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Dynamic Properties of Chondrocytes
… incorporating the simultaneous loading and imaging of cartilage tissue, to overcome common technical challenges, such as motion artifacts, which have previously limited studies on chondrocyte deformations to static conditions. Using this setup, cartilage tissues were subjected to cycles of …
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Model-based quantitative combinatorial optical microscopy for extracting clinical imaging biomarkers
… noninvasive, high-resolution biomedical optical imaging techniques based on unique, yet complementary, contrast mechanisms have emerged. While each imaging modality is able to elucidate certain properties of the particular sample under study, an integrated approach in which all modalities are …