Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"multiomic"”.
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Multiomic Atlasing of Human Mesenchyme Development
… paucity of studies applying emerging spatial and multiomic technologies to deeply map mesenchyme development in the human embryo in spatial and epigenetic contexts. Understanding the basis of cell fate decisions that drive the development of progenitors into numerous mesenchymal lineages can shed …
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Integrative multiomic insights into Alzheimer’s disease pathology
… complex I. Taken together, my work integrates multiomic data from animal models and human AD patients to gain insights into disease mechanisms, drug safety and therapeutic mechanisms. These results offer translational opportunities and call for further clinical validations to develop effective …
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Stage-Specific Multiomic Analysis of Human Erythroid Differentiation
Significance: Reduced red blood cell (RBC) production resulting in anemia is a common symptom of many diseases. RBC transfusion and erythropoiesis-stimulating agents (ESAs), primarily erythropoietin (Epo) and its analogs, are major treatments for anemia, but often lead to organ damage and/or ESA …
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Development of a framework for multiomic analysis in disease research
… studentship has highlighted the potentials for multiomics to improve disease research.
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Spatially-resolved multiomic profiling of the human heart in adulthood and development
The human heart is composed of a multitude of cell types that must work in a coordinated fashion to achieve tissue and organ functions. Single-cell genomics is a powerful framework for characterising cell and tissue biology, including functional gene programmes, regulatory networks, and cell-cell …
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Computational imaging and multiomic biomarkers for precision medicine: characterizing heterogeneity in lung cancer.
… sources, to develop computational imaging and multiomic biomarkers to characterize intratumor heterogeneity. A novel radiomic biomarker, that integrates with PDL1 expression, ECOG status, BMI, and smoking status, to enhance the ability to predict progression-free survival in a preliminary …
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Multiomic Investigation of the Human Gut Toward Insight into Childhood Inflammatory Bowel Disease Pathogenesis
Inflammatory bowel disease (IBD) is an abnormal immune response to the gut microbiome causing chronic debilitating pain in genetically susceptible people. The development of effective therapies for this condition is hampered by a poor understanding of its complex aetiology. Progress in this area will …
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Multiomic Analyses Reveal Epigenomic Alterations and New Targets of Polycomb Repressive Complex 2 in Schwann Lineage Cells and Malignant Peripheral Nerve Sheath Tumors
Malignant peripheral nerve sheath tumors (MPNST) are lethal soft tissue sarcomas occurring in 5-13% of Neurofibromatosis Type I (NF1) patients. It is thought that MPNSTs develop from benign precursors. However, the existing histologically defined stages in MPNST development need improvement. …
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Single cell -omic landscapes in normal and perturbed haematopoiesis
… describes the generation of a new single-cell multiomic atlas of healthy adult murine haematopoiesis, incorporating 200,000 single cell transcriptomes and surface proteome information from 138 antibodies. While consensus is growing over best practices for single cell transcriptomic analysis, …
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Investigation of transcriptional regulation by C. elegans SET-2/SETD1 H3K4 methyltransferase through single-nucleus profiling
… I first generated a lineage-resolved single-cell multiomic atlas of early embryogenesis in a null mutant of set-2 and then analysed the resulting alterations in gene expression and chromatin accessibility. I found extensive deregulation of diverse transcriptional programmes including shared and …
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A multiomic study to unveil the salt stress tolerance mechanisms in S. pennellii x S. lycopersicum Introgression Lines (ILs) [Uno studio multiomico per svelare i meccanismi di tolleranza allo stress salino nelle linee di introgressione (ILs) di S. pennellii x S. lycopersicum]
Lo stress salino influisce significativamente sul metabolismo delle piante, portando a uno sviluppo ridotto, a una riduzione della produzione e, infine, alla morte della pianta stessa. Molte colture hanno numerosi “parenti selvatici”, capaci di crescere in condizioni subottimali. Solanum pennellii, …
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Correlation-based Linking of Epigenomic Regions to Target Genes in Bulk and Single Cell Epigenomic Data
… linking as a key aspect of future single-cell multiomic technology, as it allows for correlation between assays at the single-UMI level. As such, improved modeling of data at the single-cell level will enhance our understanding of complex gene regulatory networks and disease mechanisms.
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INTEGRATED SINGLE-CELL MUTATION, GENE EXPRESSION AND ISOFORM ANALYSIS TO DECONVOLVE ACUTE MYELOID LEUKEMIA HETEROGENEITY
… of tumor samples, including the simultaneous multiomic analyses of both malignant and immune populations at single-cell level. In this work, we have developed a novel high-throughput multiomics approach to integrate gene mutation, expression and isoform information at single-cell resolution. …
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Regulation and function of histone variant H2BE in neurons
… of the brain. The work described here combines multiomic analyses, mouse models, and animal behavior with molecular and biochemical techniques from the chromatin biology field. Using these tools, we reveal broad expression of the H2B variant H2BE in multiple tissues, including in the brain where …
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Identification and characterisation of Pu.1-driven gene regulatory complexes and networks in Acute Myeloid Leukaemia.
… Specifically, we have compared and contrasted; Multiomic data (sc + snRNAseq & snATACseq), integrative Bioinformatic analysis (SCENIC+), qPLEX-RIME mass spectrometry analysis, proximity ligation analysis (PLA) to verify leukaemia-specific/selective interactors, co-binding by CUT&RUN analysis and …
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Μεταγραφωματική και επιγενετική ανάλυση μονήρων κυττάρων ασθενών με καρκίνο του πνεύμονα
… αλληλούχισης μονήρων κυττάρων (single-cell multiomic technologies) επιτρέπουν την ταυτόχρονη χαρτογράφηση της γονιδιακής έκφρασης και των επιγενετικών μηχανισμών σε μεμονωμένα κύτταρα. Στην παρούσα μελέτη αναλύθηκαν δεδομένα single-nucleus RNA sequencing και single-nucleus ATAC sequencing …
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Systems immunology frameworks link multicellular immune perturbation phenotypes and setpoints to response outcomes
… using top-down systems biology approaches with multiomic single cell technology to understand variation in human immune system response outcomes. We integrate human population, cell subset and single cell variations in molecular phenotypes which give rise to baseline setpoints, are perturbed by …
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MULTI-OMIC LINEAGE TRACING PREDICTS THE MOLECULAR DRIVERS OF CANCER STEM CELLS IN TRIPLENEGATIVE BREAST CANCER EVOLUTION.
… this work, we developed GALILEO, a single-cell multiomic framework that combines lineage tracing with transcriptomic and chromatin accessibility profiling (via snRNA-seq and snATAC-seq) and integrates these with phenotypic assays. Applying GALILEO to the intrinsically heterogeneous and highly …
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Somatic mosaicism in development and paediatric cancer
… the evolutionary histories of cells with multiomic approaches can reveal great insight into the paths they take from embryonic to mature or healthy to diseased, with great potential impact for both biology and clinical care of patients.
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Somatic Mutation in Cancer and Healthy Human Tissue
… in cancer. Secondly, G&T-seq, a single cell multiomics technique, is extended to single nucleotide variant analysis. When applied to cortical neurons, it suggested a substantial level of technical artefacts in previously published mutational spectra. Thus, this multiomic approach offers an …
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