Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"multi-domain proteins"”.
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Exploring the Biophysical Properties of Domains Within Multi-Domain Proteins
… in understanding the biophysical properties of proteins using small modular domains such as SH3 domains. However, there is a surprising lack of knowledge regarding how these properties are affected when the domain is placed back within its full-length multi-domain protein. Using a combination of …
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Multi-domain Protein Unfolding Pathway Studies by Single Molecule Techniques
<p>Large multi-domain proteins, which are ubiquitous in the proteomes in eukaryotic and prokaryotic organisms, still lack intensive studies on their folding mechanisms due to their complicated interactions between and inside of their domains. My work is broadly aimed at characterize folding …
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A directed evolution approach to engineering recombinant protein production in S. cerevisiae
… expression host for the manufacture of these proteins is a potential solution. Although heterologous proteins are frequently made in organisms as disparate as E. coli and bovines, the single-celled organism S. cerevisiae has emerged as a well-qualified candidate due to its approachable genetic …
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Identification of Cyclophilin gene family in soybean and characterization of GmCYP1
… in soybean (GmCYP1 to GmCYP62), of which 8 are multi-domain proteins and 54 are single domain proteins. At least 25% of the GmCYP genes are expressed in soybean. GmCYP1 localizes to the nucleus and the cytoplasm and it interacts with an R1 MYB transcription factor, GmMYB176, in the nucleus. …
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Computational methods for the analysis of protein structure and function
The vast majority of known proteins have not yet been experimentally characterized and little is known about their function. The design and implementation of computational tools can provide insight into the function of proteins based on their sequence, their structure, their evolutionary history …
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Towards unveiling the Photoactive Yellow Proteins: characterization of a halophilic member and a proteomic approach to study light responses
… of blue-light absorbing Photoactive Yellow Proteins (PYPs) has become an attractive model system to study the molecular events related to light perception. PYP covalently binds a p-hydroxycinnamic acid chromophore, which is fine-tuned for efficient light absorption and initiates a photocycle …
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Sortases: Keystones to Virulence and Targets for Anti-Infective Therapy
… in that they covalently polymerize secreted proteins, rather than attach them to peptidoglycan. Full length and truncation mutant constructs of SrtC and its substrate, T3, and peptide substrate mimics have been produced in soluble form for use in kinetic assays. Additionally, initial …