Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 58 for “"mucins"”.
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Packaging and Secretion of Airway Mucins
… cleared. This gel layer is composed of secreted mucins that become hydrated once reaching the airway lumen. Mucins are secreted at a baseline rate and at a stimulated rate, dependent on the amount of agonist. Baseline secretion is responsible for steady clearance of inhaled particles and …
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The influence of mucins on bacterial communities
… interactions. In this thesis, I demonstrate that mucins impact bacterial communities in their physical structure as well as microbe-microbe and microbe-host dynamics. In Chapter 2, 1 study the ability of mucin surface coatings to suppress bacterial surface attachment, the first step in biofilm …
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Impacts of mucins on microbial physiology and interactions
… of this thesis is to explore the effects of mucins, the main gel-forming component of mucus, on microbial behavior. Using an in vitro mucus model consisting of purified mucins, I show that these polymers suppress microbial virulence traits in selected bacterial and fungal opportunistic …
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Mucins regulate virulence and colonization factors in Streptococcus pneumoniae
… to the mucin environment. First, I identify that mucins downregulate a key virulence factor of S. pneumoniae, the cytolytic toxin pneumolysin (PLY). I show that through the regulation of PLY, mucin protects host cells from toxin-mediated killing and modulates inflammatory signals. Second, I …
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The biochemical and molecular characterisation of respiratory mucins in TB
The role of the dominant respiratory mucins (MUC5AC and MUC5B) and MUC2 has been investigated in chronic airway diseases as it is the mucin glycoprotein that confers upon mucus its biological, rheological and physicochemical properties. Within South Africa, specifically the Western Cape, TB has …
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The biochemical characterisation of respiratory mucus & mucins in normal, asthma & COPD
Airway mucus hyper-secretion is the main cause of mortality and morbidity in respiratory diseases such as asthma and chronic obstructive pulmonary disease (COPD) and is the leading cause of death in South Africa. Mucus is a viscid, slimy visco-elastic gel-like material, which coats the epithelial …
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The role of human breast milk mucus and mucins in HIV-AIDS
Milk molecules such as mucins, antibodies, bactericidal enzymes like lysozymes and fatty acids that lyse bacteria, viral particles and bacterial peptides, offer anti-microbial activity in milk. Despite human breast milk being rich in anti-microbial substances, such as mucin, that protect against …
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Separation and purification of mucins and tenascin-C in breast milk of patients and the investigation of the role of mucins and tenascin-C in the inhibition of HIV-1
… and vacuolated cytoplasm with neutral and sialomucins. The presence of MUC1, MUC4 (β subunit) and TNC were confirmed in the lactating breast tissue using immunohistochemistry.
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The biochemical analysis of mucus and mucins in respiratory diseases with a focus on tuberculosis
… The main constituents of this layer of mucus are mucins. MUC5AC and MUC5B are the predominant respiratory tract mucins. However, little is known of the association between respiratory mucins and TB. This study aimed at describing the types and role of respiratory mucins in TB. Fifty six sputum …
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Pathogenesis of Dry Eye in Graft Versus Host Disease (GVHD): Role of Ocular Mucins and Conjunctival Fibrosis
… corneal glycocalyx, a decrease in ocular surface mucins, MUC4 and MUC5AC and loss of conjunctival goblet cells. Our data showed that topical treatment by mucin secretagogue, rebamipide, partially attenuates GVHD-associated damage to ocular surface. Hyperosmolar tears are a consistent feature of …
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The role of crude saliva and purified salivary mucins in the inhibition of the Human Immunodeficiency Virus type 1
… components inhibited HIV-1 in an in vitro assay. Mucins from infected saliva did not show inhibition in this assay. Mucus was extracted in 6M guanidinium hydrochloride and a cocktail of protease inhibitors, pH 6.5. Sepharose CL-4B gel filtration separated MUC5B and MUC7 in saliva. Mucins were …
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Analyzing the Role of Mucin O-Glycans in Regulating Microbial Virulence
… Defects in the production or glycosylation of mucins, the gel-forming component of mucus, are associated with microbial dysbiosis and infection; however, owing to the complexity of mucins, an understanding of the molecular motifs and mechanisms underlying mucins’ protective functions remains …
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The effect of reduction, trypsin digestion and de-glycosylation of salivary mucins in the inhibition of human immunodeficiency virus type 1
… showed that crude saliva and purified salivary mucins (MUC5B and MUC7) inhibit HIV-1 infection in vitro. However, it is not known whether the specific arrangement of mucin carbohydrate residues is important for mucin interactions with HIV-1, or if the negative charge afforded by sialic acid and …
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Physical and chemical changes in porcine gastric mucus in the normal and ulcerated status
… CL-4B indicated larger amounts of degraded mucins relative to native mucins in the samples obtained from pre-ulcerated, ulcerated and post-ulcerated stomachs, as compared with the normal and control samples. The amounts of purified mucins obtained after isopycnic centrifugation in CsCl and …
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A study of the functional anatomy of the bovine cervix with special reference to the epithelium, mucus secretion and sperm transport
… with (1) Alcian blue (AB) at pH 1.0 (sulfomucins) and at pH 2.5 (sialomucins and sulfomucins); (2) periodic acid Schiff (PAS) (neutral mucins) and AB (pH 2.5); (4) high iron diamine (HID) (neutral and sulfomucins) with AB (pH 2.5). Additional samples were processed for ultrastructural …
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The barrier functions of the cervical mucus plugs and purified mucins against a panel of HIV-1 strains in the contexts of cell-free and cell-to-cell infections
… uterus. Our laboratory found that the cervical mucins—but not the crude cervical mucus plugs—isolated and purified from the mucus plugs inhibit HIV-1 infection in an in vitro cell-free inhibition assay. We aimed to characterize and compare the inhibition of cell-free and cell-associated HIV-1 …
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The isolation, purification, tissue localization and identification of a glycoprotein found in the crude mucus gel of patients with carcinoma of the stomach
… inorganic ions and very importantly the mucins, that impart to it its gel-forming properties. Mucins are large heterogenous polymers that are difficult to characterize by traditional biochemical methods. However mucins from different regions of the body do share common features such as a …
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STRUCTURAL AND FUNCTIONAL CHARACTERISATION OF MUCUS ADHESION PROTEINS OF LACTOBACILLUS REUTERI
… culture media, demonstrated binding to different mucins in vitro. Sugar inhibition experiments and glycan arrays suggested the involvement of sugar recognition in MUB protein binding to mucins. Lar0958 is a modular protein of six Lar0958 repeats present on the cell-surface of L. reuteri DSM …
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Unnatural glycopeptides with application on cancer research
… relevant implications to fight against cancer: mucins, such as mucin-1 (MUC1), and antifreeze glycoproteins. Concerning the first mentioned class, mucins are a family of O-glycoproteins with high molecular weight that serve as cell-surface receptors and sensors. Notably, aberration in the …
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Modulation of airway mucin glycosylation by Pseudomonas aeruginosa pyocyanin
… levels of sialyl-Lewisx modifications on airway mucins in a mouse model and in in vitro cell culture. We found PCN to be a potent inducer of sialyl-Lewisx in both mouse airways and in the cultured airway epithelial cells. PCN increases the expression of ST3Gal-IV and C2/4GnT, two enzymes …
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