Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 10 of 10 for “"mouse hepatocytes"”.
-
Lipid acquisition by apolipoprotein A-I in ER and Golgi compartments of primary mouse hepatocytes
… lipidation of newly synthesized apoA-I in hepatocytes were in plasma HDL formation. By labeling primary mouse hepatocytes with 3H-choline, we showed that phospholipidation of apoA-I is most significant in endoplasmic reticulum (ER) and medial Golgi compartments with minor lipidation upon …
-
Quantitative analysis of cell decision processes in response to inflammatory cues and their role in mediating genotoxicity in hepatocytes
… model system that can maintain primary mouse hepatocytes in a differentiated state and study the independent extracellular cues which regulate hepatocyte behavior during chronic inflammation. This model system allowed systematic variation of oxygen concentration as well as matrix …
-
Uncovering Molecular Mechanisms Regulating Circadian Amplitude Across the Tree of Life
… RORs positively regulates circadian amplitude in mouse hepatocytes, and the ROR paralog Rora has a stronger impact on amplitude than Rorc. This is due to the disordered hinge region and the ligand binding domains of RORɑ, and in silico models predict that these regions are critical for formation …
-
The potential of sphingolipid depletion for the treatment of atherosclerosis
… To address this, HepG2 cells and primary mouse hepatocytes were treated with myriocin. This significantly increased apoA-I mRNA, and protein levels. It also increased apoA-I secrection, and decreased ERK phosphorylation. These in vitro data indicate that ERK phosphorylation plays a role in …
-
Regulation of amino acid transport across the lysosomal surface by the mTORC1 pathway
… of essential amino acids from lysosomes of mouse hepatocytes in-vivo during periods of starvation and refeeding. Thus, the studies described in this thesis provide new insights to the role of the Rag GTPases by genetic, biochemical, and structural techniques. Further mechanistic insights …
-
Establishment of new human and mouse liver cancer models and their use to uncover the role of RNF43 and ZNRF3 in liver homeostasis and repair
… able to generate tumours in an in vivo xenograft mouse model after long-term expansion. Furthermore, we have shown that tumoroids can also be successfully used for drug testing, suggesting their use to devise new targeted therapy as well as personalised treatment strategies. Current models to …
-
Development of Polymeric Nanocarriers for Dual Magnetic Resonance Imaging and Drug Delivery
… and did not show any in vitro toxicity against mouse hepatocytes. The T1 relaxivities of the MaGICs were 2-10 times higher than that of a commercial manganese based contrast agent MnDPDP. These MaGICs with encapsulated anticancer drugs including doxorubicin, cisplatin and carboplatin have …
-
Alterations in Lipid Metabolism in Mouse Tissues and Hepatic Cell Lines in Response to the Trans10,Cis12-18:2 Isomer of Conjugated Linoleic Acid
… of mRNA for CPT-1 and fatty acid profile in mouse hepatocytes (AML-12) and human hepatoma cells (HepG2). In the first study, male or female mice were fed diets containing 0, 0.15%, or 0.30% trans10,cis12-CLA for 6 wk. Epididymal adipose weights (males) and inguinal adipose weights (females) …
-
Role of Src phosphorylation of FXR in bile acid regulation
… of FXR is rapidly phosphorylated in liver hepatocytes in response to treatment with either natural bile acids or FGF19. Biochemical analysis paired with bioinformatic tools identified Src as the kinase responsible for this post-translational modification. Feeding mice a diet supplemented …
-
A novel pathway for VLDL assembly in the mouse liver
Es wird davon ausgegangen, dass LDL von der Leber und von peripheren Zellen endozytiert und vollständig abgebaut wird. Mit Hilfe primärer Maushepatozyten, durch Mausleberperfusion und durch intravenöse Injektion von LDL an Mäusen wird in der vorliegenden Dissertationsschrift gezeigt, dass als LDL …