Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"mouse heart"”.
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Characterization of Murine Cardiac Cholinergic Innervation and Its Remodeling in Type 1 Diabetes.
… the distribution of autonomic nerves in the mouse heart, making it difficult to compare current findings from clinical and experimental models related to cardiovascular diseases. Furthermore, determination of the requirements for the development of this system and its maintenance in adult …
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The epigenetics and role of Dopa Decarboxylase in heart development
… imprinting in the developing and neonatal mouse heart with transcription occurring solely on the paternally inherited allele, with the maternally inherited allele epigenetically silenced. In all other tissues where Ddc_exonla is expressed transcription occurs from both parentally inherited …
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Heart Regeneration: An Evolutionary Tale
… pre-existing cardiomyocytes. The adult mammalian heart lacks any meaningful endogenous regenerative response following injury. However, embryonic mammalian cardiomyocytes are proliferative and exit the cell cycle shortly after birth. The question of whether the mammalian heart lacks this …
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Elucidation of Emergent Regional Mechanisms of Heart Muscle Dysfunction in the Mouse Model of Duchenne Muscular Dystrophy
… mishandling have not been defined in the whole heart. Here, we elucidate, for the first time, loci- and age-dependencies between calcium mishandling and myocardial sheet function as a manifestation of dystrophin-deficient cardiomyopathy. We also map calcium transients to illustrate the regional …
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Identification and Quantification of Protein Carbonylation by Mass Spectrometry
… investigate protein carbonylation in ischemic mouse heart mitochondria, and develop a quantitative method for detecting carbonylated protein in this system. The research is based on liquid chromatography/mass spectrometry (LC/MS), Western Blots, and enzymatic assay.
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The role of ischaemia-reperfusion injury and mitochondrial dysfunction in organ transplantation
… ability to moderate metabolic pathways in the mouse heart during organ storage using metabolic inhibitors. In order to investigate the effects of the metabolic changes during ischaemia on reperfusion I developed a novel, small animal model of solid organ transplantation incorporating a warm …
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Cardiomyocyte Cell Cycle, Renewal and Isolation
Heart disease results from the loss of cardiomyocytes following myocardial damage and is a leading cause of death worldwide. Stimulating the endogenous regeneration of cardiomyocytes or isolating them from in vitro culture is an attractive therapeutic strategy. In paper I, the focus is on the …
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ADVANCED MRI FOR CARDIAC ASSESSMENT IN MICE
Heart failure is a leading cause of mortality in the Western world. The mouse is a widely used model for a number of diseases, induced by genetic modification or surgical intervention. When performing experiments in mice, in vivo magnetic resonance imaging (MRI) can be used to evaluate heart …
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Novel Strategies in Cardioprotection against Ischemia/Reperfusion Injury
… and pharmacological preconditioning (PC) of the heart. Phosphodiesterase type 5 (PDE-5) is an enzyme that specifically hydrolyzes cGMP thereby decreasing its tissue concentration. Sildenafil is a potent selective inhibitor of PDE-5 and therefore allows the accumulation of cGMP in several tissues …
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Transcriptional Regulation of Neonatal Heart Regeneration and Direct Cardiac Reprogramming
The adult mammalian heart has limited capacity for regeneration following injury, whereas the neonatal heart can readily regenerate within a short period after birth. Deciphering the molecular underpinnings of neonatal heart regeneration and the blockade to regeneration in later life may provide …
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Using molecular approaches to understand Complex I deficiency in mouse models
… complex I dysfunctions remain unclear. Here, two mouse models of complex I-linked mitochondrial disease were analysed structurally by cryo-EM to understand the mechanisms of their pathogenesis. The first part of this thesis explores complex I from the ND6-P25 mouse model, which contains a …
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Developing mouse complex I as a model system: structure, function and implications in mitochondrial diseases.
… medically relevant model organism Mus musculus (mouse), in a pure, stable and active state. The enzyme from mouse heart mitochondria was then comprehensively characterised, to ensure the presence of all the expected subunits and co-factors, and to define its kinetic properties. The second part of …
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Extracellular Superoxide Dismutase Protects Cardiomyocytes During Hyperoxia
… oxygen for 6 hours and for 72 hours, and the hearts were subsequently removed under deep anesthesia. The mortality rate in KO mice after 72 hours (25%) was significantly higher than wild type littermates (10%) and TG mice (4.2%). In the EC-SOD TG mice, 6 hours of exposure to hyperoxia …
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Role of Dopamine Receptor 3 in Cardiac Fibrosis
… been removed from the market due to fibrosis and heart valve regurgitation. We hypothesized that there is an intrinsic cardiac dopaminergic system and changes to that system can lead to left ventricular (LV) remodeling and particularly, cardiac fibrosis. The first aim focused on determining the …
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Imaging 3D tissue fiber organization using optical polarization tractography
… histology comparison study was conducted using heart tissues which are known to have a depth-dependent fiber orientation change. A systematic image processing procedure was developed to register histology images with OPT images so that the pixel-wise difference between the two measurements can …
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Developing methods for measuring mitochondrial redox status and membrane potential in the isolated perfused heart
… intrinsic chromophores in the isolated perfused heart. The absorbance of mitochondrial cytochromes provides information about reducing equivalent delivery, respiratory flux, tissue oxygenation, and ΔΨ<sub>m</sub> and thus provides a wealth of information about cardiac energetics. The distribution …
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Development of the Clinical Application of miRNA with Proregenerative Effect on the Heart
… part, to the poor regenerative capacity of the heart and the lack of drugs able to foster cardiac regeneration. Our recent work has identified a few human microRNAs (miRNAs), in particular hsa-miR-199a-3p, able to stimulate proliferation of cardiomyocytes and, once expressed in the mouse heart …
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Probing Tissue Microstructure Using Susceptibility Contrast Magnetic Resonance Imaging
… susceptibility anisotropy in myocardium. Intact mouse heart specimens were scanned using MRI at 9.4 T to ascertain both the magnetic susceptibility and myofiber orientation of the tissue. The susceptibility anisotropy of myocardium was observed and measured by relating the apparent tissue …
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Submicron-resolution Photoacoustic Microscopy of Endogenous Light-absorbing Biomolecules
… high-resolution PAM could resolve capillaries in mouse ears more clearly. As an example application, we demonstrated intracellular temperature imaging, assisted by fluorescence signal detection, with sub-degree temperature resolution and sub-micron lateral resolution.</p><p>The second part of this …
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