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Showing 1 to 20 of 116 for “"mouse embryonic stem cells"”.
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Tumor-like growth of mouse embryonic stem cells
… capability of self-renewal and differentiation, embryonic stem cells (ESCs) are an invaluable tool for research in regenerative medicine. However, the main problem that hampers application of ESCs to practical stem cell therapies is that ESCs can grow as tumors when transplanted. To date, only …
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Directed differentiation of endodermal cells from mouse embryonic stem cells
Pluripotent embryonic stem cells hold a great promise as an unlimited source of tissue for treatment of chronic diseases such as Type 1 diabetes and chronic liver disease. Various attempts have been made to produce cells that can serve as precursors for pancreas and liver. By using …
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Molecular regulation of Polycomb complexes in mouse embryonic stem cells
Contains fulltext : 203027.pdf (Publisher’s version ) (Open Access)
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THE ALTERNATIVE SPLICING OF NF-YA REGULATES MOUSE EMBRYONIC STEM CELLS DIFFERENTIATION
… maintenance of self-renewal and pluripotency of Embryonic Stem Cells (ESC), others drive cell lineage decisions which give rise to the entire organism. An example of these factors is NF-Y, a heterotrimeric pioneer TF promoting chromatin accessibility to other TFs to regulate gene expression. The …
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ZSCAN4 AND REPLICATION STRESS: SAFEGUARDING GENOME STABILITY IN MOUSE EMBRYONIC STEM CELLS
… Our laboratory discovered that exposure of mouse embryonic stem cells (mESC) to RS induces the expression of Zscan4, a marker of the 2C stage in mouse embryo development. Depletion of Zscan4 in mouse zygotes reduces the number of blastocysts formed, which eventually fail to grow and implant. …
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Dicer deletion and short RNA expression analysis in mouse embryonic stem cells
… describes the short RNAs expressed in mouse embryonic stem (ES) cells. ES cell lines are derived from the pre-implantation blastocyst and can be cultured in vitro for extended periods while still maintaining pluripotency. It was demonstrated that approximately 130,000 5' phosphorylated …
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Developmental Regulation of Prion Expression in Cattle and Mouse Embryonic Stem Cells
… However, recent reports using Prnp-null mouse models have suggested the participation of PrPC in neural stem/progenitor cell proliferation and differentiation. The first objective in our study was to map the expression of PrPC in twenty six somatic and reproductive tissues in ruminants. …
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THE IMPACT OF REPLICATION STRESS ON THE EPIGENOME OF MOUSE EMBRYONIC STEM CELLS
… replication stress, can expand the cell fate of mouse embryonic stem cells (mESCs) by activating totipotency and extra-embryonic pathways. However, the underlying epigenetic mechanisms remain unclear. Preliminary data indicated that polycomb repressive complex 2 (PRC2)-targeted genes are …
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The Role of Dax-1 in Regulating Pluripotency in mouse Embryonic Stem Cells
… Dax-1 is highly expressed in pluripotent embryonic stem (ES) cells and shows a correlative reduction in expression as these cells differentiate. While it is known that Dax-1 is expressed in pluripotent mouse ES cells, the precise function of Dax-1 in these cells is not as well understood. …
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Modeling 3D Retinogenesis in Mouse Embryonic Stem Cells Following CRISPR-mediated Crx Knockdown
… that it is possible to differentiate dissociated mouse embryonic stem cells (mESCs) into 3D retinal tissues <em>in vitro</em><em> </em>(Bertacchi, 2015; Eiraku, 2012). The newly differentiated retinal tissues are said to encompass all of the major components found in retinal tissues. The …
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Cyclic loading of fibronectin coated beads induces differentiation in mouse embryonic stem cells
The role of soluble factors in determining embryonic stem cell fate has been well studied. A growing body of research indicates mechanical cues are equally important to chemical signals in eliciting cellular response, especially during the early developmental stages of embryos. Here, we seek to …
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Consequences of mitotic loss of heterozygosity on genomic imprinting in mouse embryonic stem cells
… of imprinted gene expression is disrupted in cells with uniparental disomy (UPD), which is an unequal parental contribution to the genome. I have derived mouse embryonic stem (ES) cell sub-lines with maternal UPD (mUPD) for mouse chromosome 6 (MMU6) to characterize regulation and maintenance …
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Cell-cell and cell-medium interactions in the growth of mouse embryonic stem cells
Embryonic stem cells serve as powerful models for the study of development and disease and hold enormous potential for future therapeutics. Due to the potential for embryonic stem cells (ESCs) to provide a variety of tissues for use in regenerative medicine, there has been great interest in the …
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Comparative Analysis of Antiviral Responses in Mouse Embryonic Stem Cells and in Mesenchymal Stem Cells
<p>Embryonic stem cells (ESCs) are cells that have unlimited capacity for selfrenewal and differentiation. These properties make ESCs a great cell source for application in regenerative medicine. When used for cell therapy, ESC-derived cells could be placed in a wounded area that is likely to be …
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Investigating the Role of the mRNA Capping Enzymes in Mouse Embryonic Stem Cells and Differentiation
… complex, are expressed at different levels in mouse primary tissues, and RAM is particularly highly expressed in mESCs. RAM is essential for pluripotency maintenance by controlling the expression of pluripotency-associated transcription factors in a positive feedback loop manner. During neural …
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A multi-lineage in vitro mouse embryo model derived exclusively from mouse embryonic stem cells
The mouse embryo has been a vital model to study human embryonic development and congenital diseases. As the mouse embryo implants, it undergoes drastic morphological changes to form the egg cylinder consisting of the epiblast (EPI), extraembryonic ectoderm (ExE) and visceral endoderm (VE), which …
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Defining the Transcriptional and Epigenetic Signature of Mouse Embryonic Stem Cells with Compromised Developmental Potency
Mouse embryonic stem (ES) cells have played a crucial role in studying developmental processes and gene function in vivo. They are extremely useful in the generation of transgenic animals as they can be genetically manipulated and subsequently microinjected into blastocyst stage embryos, where they …
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Promoter directionality is controlled by U1 snRNP and polyadenylation signals in mouse embryonic stem cells
… at a candidate set of divergent promoters in mouse embryonic stem cells (mESCs). We reveal that upstream antisense RNAs (uaRNAs) are less than 1 kb in size, 5'-capped, heterogeneous at their 3'-ends, and accumulate to 1-4 copies per cell at the steady state. In addition, uaRNA are transcribed …
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Fusion of bovine fibroblasts to mouse embryonic stem cells: a model to study nuclear reprogramming
The cells from the inner cell mass (ICM) of an early embryo have the potential to differentiate into all the different cell types present in an adult organism. Cells from the ICM can be isolated and cultured in vitro, becoming embryonic stem cells (ESCs). ESCs have several properties that make them …
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Attenuated Responses To Inflammatory Cytokines In Mouse Embryonic Stem Cells: Biological Implications And The Molecular Basis
<p>Embryonic stem cells (ESCs) have attracted intense interest due to their great potential for regenerative medicine. However, their immune property is an overlooked but a significant issue that needs to be thoroughly investigated not only to resolve the concern for therapeutic applications but …
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