Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"mouse embryonic stem cell"”.
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Regulated Histone H3 Proteolysis During Mouse Embryonic Stem Cell Differentiation
… chromatin often lead to dynamic changes in many cellular processes, including development and differentiation, by affecting the accessibility of the genomic information stored in the DNA. The cell uses many different mechanisms to regulate chromatin in order to establish, maintain, and propagate …
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The regulation of mouse embryonic stem cell differentiation by Nrf2
Embryonic stem (ES) cell maintenance and differentiation are dynamic processes controlled by various intrinsic and extrinsic factors. Identifying these factors will enhance the understanding about developmental process and improve the application of stem cells in clinic. Previous studies highlight …
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Effects of Trimethylamine N-Oxide on Mouse Embryonic Stem Cell Properties
… oxygen species (ROS) generation in numerous cell types, resulting in cell dysfunction. However, whether TMAO will impact stem cell properties remains unknown. This project aims to explore the potential impact of TMAO on mouse embryonic stem cells (mESCs), which serve as an in vitro model of …
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P120-Catenin Regulates Rest and Corest, and Modulates Mouse Embryonic Stem Cell Differentiation
… studied to determine their contributions to stem cell biology, but less is known about p120-catenin in the nuclear compartment. P120 is developmentally required as a consequence of its biochemical and functional interactions with cadherins, small-GTPases and transcriptional regulators. We …
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Analysis of an epigenetic regulator in mouse embryonic stem cell self-renewal and differentiation
… and widespread defects {Glaser, 2005 #296}. Thus mouse embryonic stem (ES) cells carrying a null mutation of trx2 were used as an alternative model system to address the implication of Trx2 in differentiation. This study showed that Trx2 is redundant for ES cell self-renewal. Homozygous trx2 …
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Transplantation of Mouse Embryonic Stem Cell-Derived Dopaminergic Neurons in a Unilateral 6-Hydroxydopamine Lesion Rat Model of Parkinson’s Disease
Embryonale Stammzellen (ES Zellen) sind eine vielversprechende Möglichkeit für Zellenerzatztherapie der Parkinson-Erkrankung. Undifferenzierte ES Zellen der Maus differenzieren sich zu funktionellen dopaminergen Neuronen nach der Transplantation in ein experimentellen Rattenmodell der …
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Characterization of spontaneous and evoked activity of mouse embryonic stem cell derived motor neurons using optogenetics and micro electrode array electrophysiology
… neuronal lineages derived from progenitor cells is essential for the development of biological models that can recreate native function of neurons of the central nervous system while permitting researchers to have an easily accessible source of cellular building blocks. These models are …
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An exploration of the molecular mechanisms underlying the effects of hyperglycaemia on the autorrhythmicity of cardiac-like stem cells
… poorly understood. Using a cardiac developmental cellular model, this study aimed to explore the effects of hyperglycaemia on the autorrhythmicity of mouse embryonic stem cell (mESC)-derived cardiac-like cells.
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The cell-secreted microenvironment : shaping embryonic stem cell self-renewal and differentiation
… is to obtain an in depth understanding of how embryonic stem cell-secreted signals contribute to their identity. We analyze the contribution of broad and specific signals present in the cell-secreted microenvironment using techniques that can easily be applied to studies of other cell types and …
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Studies of the MYM-type zinc finger protein ZMYM3
… wild-type and mutant RNase H2B in HEK293T cells. In this work, I identify several members of a putative chromatin-silencing complex as novel RNase H2B binding partners whose binding is impaired by each mutation in this cluster, including: HDAC2, a histone deacetylase, LSD1, a histone …
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MES-DERIVED CARDIOMYOCYTES AS CELLULAR MODEL TO STUDY CARDIAC DISEASES: ALTERATIONS IN STRN AND GNB5 GENES
… of CMs, generated from STRN-KO and isogenic WT mouse embryonic stem cell (mESC) lines. 10-12-day old beating mESC-CMs were analyzed by Patch-clamp, motion video tracking, Ca2+ dynamics and immunofluorescence analysis. STRN-KO cells have a higher spontaneous beating rate and faster action …
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Investigating the role of Neuronatin in neural development
… is converted into neural tissue. However, the cellular and molecular mechanisms that govern this process are still not completely understood. For instance, in Xenopus, calcium signalling is known to play a pivotal role in neural induction but whether a similar mechanism occurs in mammals is …
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Transcriptional Regulation by DAX-1 in Pluripotent and Differentiated Cells
… in of DAX-1 into a low-DAX-1 expressing cancer cell line has been previously observed to increase apoptosis, while, inversely, down in a high-DAX-1 expressing cancer cell line shows a decrease in apoptosis. Target genes that belong to the TNFα and BCL-2 families have shown changes in expression …
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Using single cell transcriptomics to characterise endothelial populations in embryonic haematopoiesis
Haematopoietic stem cells (HSCs) possess the unique ability to reconstitute an animal’s entire haematopoietic system. As such, it is inherently interesting to the fields of regenerative medicine and developmental biology to understand how these cells first form in the embryo. Haematopoietic stem …
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Context-dependent activity of a pioneer transcription factor, ASCL1, in early development
Transitions from one cell type to another are fundamental to developmental biology and they require the concerted activity of transcription factors (TFs) to ensure the expression of the right genes at the right time. Some TFs, so-called pioneer TFs, direct cell identity by opening previously closed …
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Liposome-Mediated DNA Transfer
… pressure to increase transfection efficiency in cell lines as a potential way to produce transgenics through embryonic stem cells. The second method used artificial insemination with liposome-treated sperm in an effort to produce transgenic animals. The effects of high pressure on cell viability …
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Cyclic loading of fibronectin coated beads induces differentiation in mouse embryonic stem cells
The role of soluble factors in determining embryonic stem cell fate has been well studied. A growing body of research indicates mechanical cues are equally important to chemical signals in eliciting cellular response, especially during the early developmental stages of embryos. Here, we seek to …
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Developing High Purity Embryonic Stem Cell-Derived V2a Interneurons for In Vitro Investigation and Transplantation Following Spinal Cord Injury
… lesion to activate pre-existing motor pathways. Cell replacement strategies and growth factor delivery platforms often attempt to enhance these natural mechanisms of regeneration. The work of this thesis sought to overcome the major obstacle to manipulating specific interneurons for therapy: an …
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Germ lineage specification from a pluripotent primitive ectoderm-like substrate: a role for cell-cell contacts.
… development a small number of pluripotent cells proliferate and differentiate to give rise to all the mature cell types of the organism. Among the earliest differentiation events is the process of gastrulation, in which pluripotent primitive ectoderm cells form the three germ lineages, …
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A multi-lineage in vitro mouse embryo model derived exclusively from mouse embryonic stem cells
The mouse embryo has been a vital model to study human embryonic development and congenital diseases. As the mouse embryo implants, it undergoes drastic morphological changes to form the egg cylinder consisting of the epiblast (EPI), extraembryonic ectoderm (ExE) and visceral endoderm (VE), which …
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