Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 69 for “"mouse embryonic fibroblasts"”.
-
Redox Regulation of Akt Phosphorylation in PTEN-/- Mouse Embryonic Fibroblasts
… the course of this study, it was noticed that in mouse embryonic fibroblasts that do not express the tumour suppressor PTEN (MEFPTEN-/- cells), a decrease in the intracellular level of O2.- abrogated the hyperphosphorylation of Akt that was observed in these cells. Therefore, we hypothesize that …
-
A dissection of class I phosphoinositide 3-kinase signalling in mouse embryonic fibroblasts and prostate organoids
… Past work has indicated that, in PTEN-null mouse models of prostate tumour progression, either PI3Kβ or PI3Ks α and β, have important roles. Furthermore, the cell types and mechanism involved remained unclear. Recent published work in the host laboratory had indicated that there is an …
-
The role of transcription factors in somatic cell nuclear reprogramming by eggs and oocytes
… of terminally differentiated somatic cells to an embryonic state and gain totipotency (Gurdon et al., 1958). Additionally, induced pluripotency is applied to transform identities of somatic cells to induced pluripotent stem cells by overexpression of combinatorial Yamanaka factors (iPS, Takahashi …
-
Interpreting Raman spectra using machine learning: towards a non-invasive method of characterizing single cells
… and the gene expression values of ten genes in mouse embryonic fibroblasts (MEFs) undergoing a dynamic cellular reprogramming process. Using a multi-modal, supervised learning approach, we provide evidence that Raman spectra can accurately resolve microbial cell types. This thesis also presents …
-
Novel Small Molecule Induces Apoptosis in Malignant Peripheral Nerve Sheath Tumors of Neurofibromatosis Type I
… we exploited a robust genetically-engineered mouse model of MPNST that recapitulates human NF1 associated MPNST to identify a novel small chemical compound that inhibits tumor cell growth. Compound 21 (Cpd21) inhibits growth of all available in vitro models of MPNST and human MPNST cell lines, …
-
Direct Reprogramming Of Fibroblasts Into Muscle Or Neural Lineages By Using Single Transcription Factor With Or Without Myod Transactivation Domain
… by overcoming the ethical concerns surrounding embryonic stem (ES) cell research. However, iPSC technology presents several safety concerns, such as the potential risk of tumor formation, that have caused apprehension for use of iPSCs in humans. One such approach is "direct reprogramming" which …
-
Regulation of the Arf tumor suppressor by E2F transcription factors
… cell cycle-dependent transcription. In wildtype mouse embryonic fibroblasts, the Arf promoter is specifically repressed by only one E2F isoform, E2F3, and this binding does not fluctuate during cell cycle progression. In response to oncogenic stress, such as expression of the oncoproteins c-Myc …
-
Engineering functional blood vessels in vivo
… cells (HUVECs) and 10 T 1/2 cells, a line of mouse embryonic fibroblasts. To translate this concept to the clinic, we need to utilize cells that can be secured and used in clinic. To this end, we systematically replace each individual vascular cell type with a readily available source of …
-
Identification of novel proteins that regulate the amino acid-sensitive mTORC1 pathway
… also characterized RagA-null mice and RagA-null mouse embryonic fibroblasts (MEFs). RagA is required for embryonic development, and, surprisingly, its deletion in adult mice leads to an expansion of monocytes. MEFs derived from RagA-null embryos display atypical, nutrient-insensitive mTORC1 …
-
Beta 3 integrins : negative regulators of angiogenesis
… or any of its derivatives. Nor do they occur in mouse embryonic fibroblasts which do express beta 3 integrin. The inhibition by tumstatin also occurs in in vivo angiogenesis assayed using a Matrigel plug insert. Similarly to in vitro assays, tumstatin failed to inhibit angiogenesis in beta 3 …
-
Gsk3 Mediates Signaling Upstream of Akt
… of GSK3 in MDAMB231 breast cancer cells and in mouse embryonic fibroblasts (MEFs) abrogated induction of Akt phosphorylation by the Akt catalytic domain inhibitor A674563. Our discovery that GSK3 can function upstream of Akt led us to explore the role of GSK3 in mediating signaling from growth …
-
Lack of Inflammatory Response in Mouse Embryonic Cells to Listeria Infection
… research has shown that key cell types within mouse blastocysts, including embryonic stem cells (ESCs) and trophoblast stem cells (TSCs), exhibit remarkable resilience against the cytotoxic effects associated with viral infections and embryotoxic cytokines, such as TNFα and IFNγ, both of which …
-
The role of Xist RNA in the maintenance of X chromosome inactivation
… GFP transgene, can be quantitatively assessed. Mouse embryonic fibroblasts derived from mice carrying the conditional Xist mutation were cultured and infected with an adenovirus vector carrying the gene for Cre recombinase. After Cre mediated deletion of Xist, the inactive X remained …
-
The role of E2F·pocket protein repressive complexes in cell cycle control and differentiation
… p107 and p130, were generated. Analysis of mouse embryonic fibroblasts derived from mutant embryos revealed that while loss of E2F4 alone did not lead to defects in cell cycle control, it did significantly enhance the ability of cells to differentiate into adipocytes. This phenotype could be …
-
Variation in chromosomal aberration induction in differing ATM and ASPM genotypic backgrounds detected via a modified PNA FISH technique
… was used to detect a heightened sensitivity in mouse fibroblasts in response to ionizing radiation with Atm-/- and in mouse embryonic fibroblasts (MEFs) in response to loss of Aspm gene respectively. For Atm analysis, four commonly used inbred mouse strains were used, C57BL/6J, A/J, 129S6, and …
-
Chemical Genetic Studies of Tumor Suppressors in the Developing Zebrafish
… that they are able to perform their function on mouse embryonic fibroblasts in vitro. Finally, I sought the role of the DNA damage marker H2AX in early zebrafish development. While studying the DNA damage response in cdc25a mutants, I discovered that H2AX is present and cyclic in …
-
New Targets of p53 Regulation: From Aging to Immune Response
… the transcriptome of p53+/+ and p53-/- mouse embryonic fibroblasts (MEFs), I identified Mmergln-int among the top genomic loci differentially expressed between p53+/+ and p53 -/- cells. Mmergln-int is a murine endogenous retrovirus that is transcriptionally upregulated in response to …
-
Role of JunB in hypoxia-mediated cell response and tumour angiogenesis
… signals. As loss of functional JunB results in embryonic lethality the aim of this work was to elucidate molecular mechanisms underlying this phenotype. Therefore, a comparative analysis of wildtype and junB-/- cells was performed using in vitro systems such as mouse Embryonic Stem (ES) cells …
-
The function of E2F6 in the Polycomb complex
… have generated and characterized E2f6'- mice and mouse embryonic fibroblasts (MEFs). The mutant mice are viable and survive into adulthood with similar lifespan as their littermate controls. Furthermore, the E2f6 null MEFs are indistinguishable from wild-type MEFs in asynchronous proliferation, …
-
Investigating the Molecular Mechanisms of SNED1 Fiber Assembly in the Extracellular Matrix
… First, I established an in-vitro system using mouse embryonic fibroblasts to study the mechanisms of ECM assembly. Using this system, I found that SNED1 requires the presence of two ECM proteins, fibronectin and collagen I, for its fibrillar assembly. In addition, using biolayer interferometry, …
Page 1 of 4