Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 65 for “"mouse embryo"”.
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Mouse Embryo Development in the Presence of Capsaicin
… cross the blood/placenta barrier and affect an embryo in utero. In order to assay the potential for toxicity to human embryos, mouse embryos were exposed to capsaicin and the effect of the capsaicin on embryo development was measured. Embryos were co-cultured in Krebs medium with 1% ethanol and …
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Morphogenesis of the early post-implantation mouse embryo
… that give rise to the early post-implantation mouse embryo (egg cylinder) have not been thoroughly studied and our knowledge is restricted to “snap-shot” descriptions of embryos recovered at different stages of implantation from the mother. A central feature of the egg cylinder is the …
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Characterization of serum-induced CYP1A1 expression and activity in mouse embryo fibroblasts
The AhR is a ligand-activated transcription factor that mediates the toxic effects of environmental contaminants that include TCDD. Using a TCDD dose-response treatment in MEFs, we observed a super induction of CYP1A1 with newborn calf serum (NCS) in the presence (10nM/15nM) of TCDD. In addition to …
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Attempts to localize the replicative form of polyoma virus DNA in mouse embryo cells
… found primarily in the nucleus of Py-infected mouse embryo cells. Very little Py I or II* is found in the nucleolus or in the cytoplasm. These two forms of Py DNA are located in the same sub-nuclear fraction. At least half of each is not in association with the nuclear membrane. Possible …
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Mechanisms behind the fate of early chromosomal and transcriptional heterogeneities in the mouse embryo
A series of events during the first four days of mouse embryo development leads to the formation of a blastocyst. The blastocyst consists of neatly segregated three lineages: the epiblast (EPI), which will form the fetus, the extra-embryonic primitive endoderm and the outer layer of the …
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The role of polarisation in the first cell fate decision of the mouse embryo
A fundamental process in embryonic development is the first cell fate decision, when cells take on distinct lineage identities for the first time. In mammals, this separates embryonic inner cell mass (ICM) from extra-embryonic trophectoderm (TE) during pre- implantation development. In the mouse, …
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A multi-lineage in vitro mouse embryo model derived exclusively from mouse embryonic stem cells
The mouse embryo has been a vital model to study human embryonic development and congenital diseases. As the mouse embryo implants, it undergoes drastic morphological changes to form the egg cylinder consisting of the epiblast (EPI), extraembryonic ectoderm (ExE) and visceral endoderm (VE), which …
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Investigating the mechanisms and the temporal regulation of the first cell polarity establishment in the mouse embryo
Embryonic cells of many species polarise and the cell polarity is often important for the normal developmental progression. In the mouse embryo, the prototype of epithelial cell polarity, namely apico-basal polarisation, become established at the 2.5 days’ post-fertilisation, when the embryos are …
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The Effect of Infection with Polyoma Virus on the Protein, RNA, and DNA of Mouse Embryo Cell Cultures
<p>In four separate experiments mouse embryo cell monolayers infected with polyoma virus and uninfected control mouse embryo cells were maintained in Eagle’s Minimum Essential Medium for periods of time ranging from 0 to 168 hours. The cell layers were removed from the glass by trypsinization. …
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Date with Destiny: Genetic and Epigenetic Factors in Cell Fate Decisions in Populations of Multipotent Stem Cells
… bipotent progenitors in the context of the mouse embryo. The first model aimed to explore the effects of chromatin-associated factors on the ability of pluripotent mouse Embryonic Stem Cells (ESCs) to self-renew, via monitoring gene expression heterogeneity of key genes. The second model …
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Utilising embryonic and extra-embryonic stem cells to model early mammalian embryogenesis in vitro
… mammalian development to term requires that embryonic and extra-embryonic tissues communicate and grow in coordination, to form the body. After implanting into the uterus, the mouse embryo is comprised of three cell lineages: first, the embryonic epiblast (EPI) that forms the embryo proper, …
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Regulation of polycomb repressive complexes at the neural differentiation gene Pax6
… cord neural tissue in the posterior of the embryo is progressively generated from a pool of bipotent progenitors of the stem zone/caudal lateral epiblast, termed neuromesodermal progenitors. Neural differentiation gene transcription begins in a sharply defined position due to loss of …
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Molecular Regulation of Direct Cardiac Reprogramming
… process in three different types of fibroblasts (mouse embryo, adult cardiac, and tail tip). Approximately 50% of the reprogrammed mouse embryo fibroblasts displayed spontaneous beating after 3 weeks of induction by AKT1 plus GHMT (AGHMT). Furthermore, AGHMT evoked a more mature cardiac phenotype …
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The role of inherited determinants and embryonic-extraembryonic cell interactions in models of mammalian embryogenesis
Mammalian embryogenesis begins with the fusion of an oocyte and a sperm to create the first cell of the newly developing embryo called the zygote. The mechanisms by which this single cell generates a fully formed organism have long been the subject of study of experimental biologists. Initially, …
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A study into the biology of mESCs exiting naïve pluripotency and technology development for subcellular proteomics
… have been fascinated by different aspects of embryology for decades. One of those aspects is pluripotency, the ability of embryonic cells of certain developmental stages to divide indefinitely without the loss of their potential to differentiate into derivatives of the three primary germ …
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Estrogen Regulation of Gene Expression and Analysis of the Role of the Coregulator, Repressor of Estrogen Receptor Activity (Rea)
… and changes in E2-responsiveness. Studies using mouse embryo fibroblasts (MEFs) revealed that REA heterozygous MEFs displayed a greater transcriptional response to E2. These studies demonstrate that REA is a significant modulator of estrogen responsiveness in vivo.
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The role of mir-290-295 in murine embryonic and germ cell development
… a mammalian-specific miRNA cluster that, in the mouse, is expressed specifically in early embryos and embryonic germ cells. This thesis examines the in vivo consequences of targeted deletion of mir-290-295 in the developing mouse embryo. Mir-290-295 deficiency results in a partially penetrant …
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Modelling Embryonic Haematopoiesis Using In Vitro State-of-art Gastruloid Culture
… known as a gastruloid, can mimic gastrulation in embryogenesis, which is crucial to the emergence of HSPC precursors, highlighting the potential to develop this protocol as an in vitro platform for haematopoietic research. This project aims to adapt the previously published gastruloid culture …
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Mechanisms of toxicity in cell models of FSHD
… DUX4 expression normally occurs during early embryonic development, ectopic DUX4 expression is highly toxic in human cells. In my thesis work, using a new cell model with regulated expression of DUX4, I found that expression of DUX4 leads to the stabilization of normally rapidly turned-over …
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Integrin signalling in pluripotent cells acts as a gatekeeper of mouse germline entry
… appear at the proximal posterior region of the mouse embryo, where the somatic germ layers begin to emerge and a series of dramatic morphological changes occur at the onset of gastrulation. While the transcriptional and epigenetic mechanisms that drive entry into the germline have been …
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