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Showing 1 to 8 of 8 for “"motility assay"”.
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Molekulares Tuning des Aktin-Myosin-Motors : Entwicklung und Anwendung eines Motility Assays zur Messung maximaler Verkürzungsgeschwindigkeiten intakter Muskelfasern in einem in vitro Modell
Das Motility Assay ist ein in vitro Modell zur Visualisierung der Bewegung von einzelnen Aktinmolekülen über eine myosinbedeckte Oberfläche mittels Epifluoreszenzmikroskopie. Als einfachstes Muskelmodell war es damit bisher jedoch nicht möglich, die eklatante Diskrepanz zwischen in vivo und in …
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Interactions of Smooth Muscle Myosin Filaments with Actin and Myosin Light Chain Kinase: Insights into the Mechanics of Smooth Muscle Contraction
… acto-myosin interactions is using the in vitro motility assay. Previous research investigating the kinetics underlying the interaction of soluble sub fragments of smooth muscle myosin with actin filaments showed that this is a detachment-limited model, with ADP-release from myosin being the rate …
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The effects of force on actin-myosin ATPase activity
… actin-myosin ATPase activity using a standard motility assay and analysis for skeletal and cardiac muscle myosin. A mechanical load was imposed on a sliding actin filaments using alpha-actinin, and the corresponding effects of load were determined using a novel experimental and theoretical …
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Effects of Actin-Myosin Kinetics on the Calcium Sensitivity of Regulated Thin Filaments
… kinetics. To test the model, we use an in vitro motility assay to measure the myosin-based sliding velocities of thin filaments at different pCa, N.katt, and kdet values. We observe that the combined effects of varying pCa, N.katt, and kdet are accurately fit by the analytical model. The model …
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The Effects of Treating Dauer C. Elegans with Natural Anthelmintics and Ivermectin
… and Ivermectin were used to treat the worms. A motility assay was conducted before, during, and after the treatments. As a result, the wormwood extract was effective at killing both wild and odr-3 mutant strains in either dauer or non-dauer worms. The wormwood extract appeared to be the most …
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Regulation of Cell Migration by WNK1
Cell motility is an immensely complex process that involves reorganization of the cytoskeleton, and consequent membrane deformation, triggered by a variety of motogenic stimuli, including growth and chemotactic factors, hormones, and elements of the extracellular matrix. My graduate research has …
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Non-equilibrium dynamics of adsorbed polymers and filaments
… part, we consider collective filament motion in motility assays for motor proteins, where filaments glide over a motor-coated substrate. The model for the simulation of the filament dynamics contains interactive deformable filaments that move under the influence of forces from molecular motors …
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The regulation of the T-box transcription factor TBX3 in Luminal A breast cancer
… rescued TBX3 levels. Lastly, cycloheximide chase assays and western blotting show that phosphorylation of TBX3 by AKT1 at Serine 720, stabilizes TBX3 in breast cancer cells, and mutating this site affects its ability to promote breast cancer cell migration. This suggests that the putative AKT …