Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 61 for “"morpholino"”.
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DESIGN AND SYNTHESIS OF ALPHA-AMINO ACID AND MORPHOLINO `CHIMERA' BUILDING-BLOCKS
… Penso Design and synthesis of -amino acid and morpholino ‘chimera’ building-blocks Non-natural quaternary α-aryl-α-amino acids are of great interest for their potential intrinsic bioactivity.1 here we reported the stereoselective conversion of a series of N-aryl-sulphonyl-α-amino acid t-butyl …
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Electronic absorption spectra of basic dyes containing terminal diethylamino and morpholino groups
Comprehensive series of methyl and triuluoromethyl derivatives of Brilliant Green and Morpholine Green have been synthesised and their visible absorption spectra have been correlated with structural features. The spectral data for the parent dyes have been compared with those for Malachite Green …
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Molecular Profiling: Gene Expression Reveals Discrete Phases of Lens Induction and Development in Xenopus Laevis
… manner, including the optic cup and the lens. Morpholino-mediated knockdown of Psf2 resulted in dosage-dependent phenotypes which included microphthalmia, coloboma, and abnormal lens formation. Additionally, tissue manipulation experiments in conjunction with morpholino injections demonstrated …
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Characterisation and functional analysis of a novel msp domain – containing protein, MOSPD1
… Mospd 1 gene expression was knocked down using morpholino anti-sense knockdown technology. A morpholino was generated against the splice-site between exons 2 and 3 of the zebrafish Mospd 1 gene and injected into early embryos. At doses that significantly reduced the level of Mospd 1, to below 50 …
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ELUCIDATING THE ROLE OF MIR-10A/B IN THE WOUND HEALING PROCESS IN LARVAL ZEBRAFISH
… can rescue the regenerative defect caused by morpholino-mediated knockdown. In addition, miR-10 participates in cellular proliferation without altering apoptosis during the healing process, further eliminating off-target concerns for the morpholino. miR-10 also influences the formation of …
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Epigenetic therapy based on micrornas inhibition for the amelioration of masld phenotype
… several systems to inhibit microRNAs and RNAs, morpholino is a form of antisense RNA inhibition used to block complementary RNA sequences. Considering the potential pathological influence of microRNAs in MASLD, we aimed to discover and describe the role that several microRNAs may have on MASLD, …
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The Chemistry of some Di- and Tri-Phenylmethane dyes
… diethylamino, pyrrolidino, piperidino, morpholino, thiomorpholino and N-methylpiperazino. The rates of ailcalirie hydrolysis of the dyes at various hydroxide ion concentrations and temperatures have been investigated with a view to elucidating the reaction mechanism and the nature of …
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Towards Enzymatic Synthesis of Uncharged Xeno Nucleic Acids
… one of the non-bridging backbone oxygens, and morpholino nucleic acids, which carry a six-membered morpholine ring as well as dimethylamino or alkyl substituents on the backbone. The choice of these two chemistries is motivated by the hypothesis that such uncharged genetic polymers should …
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Molecular and Pharmacological Studies of Alpha1-AR Subtypes and their Signaling Pathways
… backbone modification (phosphorothioate vs. morpholino) altered the potency of the antisense oligomer sequence characterized above. I measured ccib-AR density in [3H]-prazosin saturation binding experiments using membranes from cells treated with varied concentrations of either …
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Role of Xenopus Pitx3 during early development.
… Similar effects are obtained using antisense morpholino oligonucleotide-mediated translation knockdown. Reciprocal grafting experiments using wildtype and morpholino treated tissues demonstrate that xPitx3 in the presumptive lens ectoderm is required for both lens and retina formation. Ectopic …
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FUNCTIONAL ANALYSIS OF ANAPLASTIC LYMPHOMA KINASE DURING EMBRYONIC NEUROGENESIS IN ZEBRAFISH
… neurons. Loss-of-function in alk by, Morpholino knock-down and a small molecule inhibitor compromised neuronal differentiation and neuron survival in the CNS, but left progenitor formation intact. Also Midkine, a putative ligand for Alk and receptor protein tyrosine phosphatase zeta …
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Signaling mechanisms and developmental function of fibroblast growth factor receptors in zebrafish
… range. Through TILLING mutant recovery and morpholino knockdown studies we also show that Fgfr2 is required for growth and skeletal development in zebrafish, whereas Fgfr4 is required for pectoral fin specification and growth.
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Aquaglyceroporin Expression and Regulation in Erythrocytes From Freeze Tolerant Cope’s Gray Treefrog, <i>Hyla Chrysoscelis</i>
… knockout tools, a novel method of antisense HC-3 morpholino delivered in to cultured suspension erythrocytes via a peptide mediated Endo-Porter was developed where HC-3 protein expression was reduced by 94% in morpholino targeted cells (as assessed by Western blotting) as compared to controls. In …
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Identification and Functional Characterization of Novel Genes Involved in Primary Neurogenesis in Xenopus laevis
… Funktionsverlust, der durch Verwendung eines Morpholino-antisense-Oligonukleotids erzeugt wurde, die Bildung von primären Neuronen und induzierte eine gesteigerte Zellproliferation. Diese Hemmung der Neurogenese wurde durch Koinjektion von XPak3-myr wieder aufgehoben. Schlussfolgernd schlagen …
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Role of ANLN in post ischaemic angiogenesis
… Hypomorphic modulation of anln expression by morpholino injection of flk:gfp embryos did not alter the vascular morphology of the 2-3 dpf zebrafish vasculature. This data for the first time associates ANLN with the EC response to the angiogenic response in ischaemia. Although our zebrafish …
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Potential roles for Elf3 in fetal alcohol spectrum disorder and development
… zygotic genome activation. Translation blocking morpholino oligonucleotides were used to implicate Elf3 in epiboly movements during gastrulation and zebrafish tail development. Taken together these results help to strengthen the zebrafish as a model for FASD in addition to giving greater insight …
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Identifizierung neuer vegetal lokalisierter RNAs in der Xenopus laevis Oozyte und deren funktionelle Charakterisierung
… der XDead end Proteintranslation druch Morpholino Oligonukleotid Injektionen resultiert im Ausbleiben einer korrekten Migration der Keimzellvorläuferzellen und deutet auf eine konservierte Funktion XDead ends innerhalb der Keimzellentwicklung hin. In Mikroinjektionsexperimenten konnte …
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FUNCTIONAL CHARACTERIZATION OF DENND5A/RAB6IP1 IN EARLY EMBRYONIC DEVELOPMENT
… in-vivo experiments, knockdown of Dennd5a by morpholino leads to unclosed blastopore and “open-back” defect during embryonic development. Interestingly, overexpression of Dennd5a by microinjection of RNA leads to very similar phenotype.
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The Role of Kitlb on Development of Coordinated Muscular Contractions in the Zebrafish Gastrointestinal Tract
… on the development and maturation of ICC using morpholino oligonucleotides knockdown in zebrafish. Gene expression was quantified using reverse transcriptase PCR analysis. Digital imaging techniques was used to examine morphology of the GI tract. It is anticipated that continued stimulation of …
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Establishment of retinoic acid gradients in the early development of Xenopus laevis
… durch die Verwendung spezifischer Antisense Morpholino Oligonukleotide zeigt den gegenteiligen Effekt in Bezug auf die Genexpression von Markern der dorsalen Blastoporuslippe. Zusätzlich ist eine Ventralisierung der Embryonen und eine Anteriorisierung des sich entwickelnden Gehirns zu …
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