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Showing 1 to 16 of 16 for “"monoploids"”.
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Derivation, fertility and breeding value of doubled monoploids from the diploid potato species, Solanum phureja
Thirty-two monoploids (<i>2n = x = 12</i>) derived by anther culture of ten diploid clones of <i>Solanum phureja</i> were used to generate doubled monoploids through in vitro shoot regeneration. Doubled monoploids were compared to the anther donor and progenitor monoploids for morphological …
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Derivation and Comparison of Androgenic and Gynogenic Monoploid Potato Families
… IVP101 seeds, 278 were spotless, resulting in 27 monoploids. Approximately 37 monoploids were obtained from the 99,500 BARD1-3 x IVP101 seeds of which 500 were spotless. In anther culture, PP5 and BARD1-3 yielded 0.16 and 1.67 embryos per anther respectively of which 51% and 44% of the regenerants …
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Application of molecular markers to characterize potato plants derived from anther culture and protoplast fusion
… a selection against lethal recessive genes. Monoploids carry only a single set of chromosomes and every locus carries only one allele. So the surviving monoploids are devoid of lethal alleles due to the effect of the "monoploid sieve" Protoplasts are plant cells, the cell walls of which have …
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Utilization of tissue culture methods and molecular markers for improvement of Solanum phureja Juz. & Buk.
Anther-derived monoploids of Solanum phureja were utilized to investigate factors essential to an efficient method of regenerating doubled monoploids (DMs). The presence of silver thiosulfate (STS) in the MS basal medium did not affect the frequency of cells with 2x nuclei but increased the …
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Inheritance of protoplast culturability and improvement in pollen development by protoplast manipulation in solanum
… resynthesizing superior diploids from selected monoploids, and tetraploids from selected diploids and dihaploids. Successful application of somatic hybridization largely depends upon protoplast culturability and regenerability of a plant. The ability of callus formation and plant regeneration …
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Studies of androgenic processes in diploid potato
… from anther donor (2n=2x=24) and anther-derived monoploids (2n=1x=12). Anther donor and anther-derived monoploids were distinguished by scoring segregating bands as well as total number of scorable bands that exhibited polymorphism. Thus RAPD has the potential to separate homozygous from …
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AFLP Marker Analysis Of Monoploid Potato
… populations, ranging from 35% in the gynogenic monoploids (GM) to 46% in the androgenic monoploids (AM). Genetic diversity appeared more random among the monoploid populations after skewed marker data was removed from phylogenetic analyses. Bilateral and unilateral marker skewness in the …
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Embryogenic response of potato anther culture to colchicine
… derived by crossing six anther-derived doubled monoploids with three heterozygous pollinators. A mean of 1.78 embryos per anther was obtained; again, there was no significant difference among the colchicine treatments, which ranged from 1.27 to 2.75 for 50 mg/L colchicine and control, …
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Analysis of anther-derived plants of Solanum phureja: variation in ploidy, photosynthetic efficiency and structure of the nuclear genome
… or by cross-pollination between fertile doubled monoploids. Within this framework, the objectives of this research were to analyze variation among anther-derived plants of Solanum phureja regarding their: 1) ploidy level and morphology, 2) net photosynthesis and its biochemical components, and 3) …
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Development of intermonoploid somatic hybrids of potato and their molecular analysis based on polymorphism for retroelement Tst1
… cross-pollinating species. Four families of monoploids (2n=1x=12), developed from diploid hybrids with diverse genomic constitutions but heavily favoring Solanum phureja, a primitive cultivated potato, were used in electrofusion experiments to create intermonoploid somatic hybrids (SH). The …
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Protoplast Fusion for the Production of Intermonoploid Somatic Hybrids in Cultivated Potato
… a population of more than 100 anther-derived monoploids. Chemical fusion and electrofusion between pairs selected from 31 superior monoploids resulted in the production of three different groups of intermonoploid somatic hybrids. The hybridity of somatic hybrid plants and calluses was …
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Derivation of interspecific Solanum hybrid genotypes with resistance to Colorado potato beetle (Leptinotarsa decemlineata Say)
… and the regeneration potential of anther-derived monoploids was evaluated. In vivo evaluation of interspecific hybrids was also performed. Three hybrids were anther cultured to observe the effects of reduced nitrogen source on androgenesis. Anthers were distributed to five reduced nitrogen …
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Use of monoploid solanum phureja in cell and tissue culture techniques for potato improvement
… anther-donor plant, one of its anther-derived monoploids, and a diploid and tetraploid plant derived from callus culture of the monoploid genotype. Flow-cytometric analysis of propidium-iodide Stained cells and nuclei showed a greater ploidy stability for plant material grown in vitro and a …
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Factors affecting variability in anther culture and in regeneration of androgenic embryos of Solanum phureja
… were performed on several anther-derived monoploids of S. phureja to examine the frequency of nuclei at the 1x, 2x, and 4x levels within and among clones. Significant variation was found among duplicate cultures of the individual clones, but this variation was small enough to allow the …
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Molecular marker analysis of a segregating monoploid potato family
… homozygous diploid plants that were added to the monoploids. In total 34 monoploid plants and 14 homozugous diploids were obtained. The degree of heterozygosity revealed by SSR analysis indicated that the diploid plants originated from unreduced gametes formed by first division restitution (FDR) …
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Bulk segregant analysis for anther culture response and leptine content in backcross families of diploid potato
… 2) embryo regeneration rate and 3) percentage of monoploids (2<I>n</I>=1<I>x</I>=12) among regenerants. CP2 was intermediate, 80-1 was low, and 1-3 was high for ACR. Only EPA was selected for further characterization in our populations. PBCp (78 genotypes) and CBC (57 genotypes), were …