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Showing 1 to 15 of 15 for “"monoploid"”.
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AFLP Marker Analysis Of Monoploid Potato
… conducted on two sibling S. phureja "BARD 1-3" monoploid populations derived by androgenesis in anther culture, and gynogenesis through the use of a haploid-inducing pollinator, S. phureja "IVP 101." Little indication of somaclonal variation and haploid-inducer gene introgression was found in …
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Molecular marker analysis of a segregating monoploid potato family
… experiments were conducted to construct a monoploid family. The donor plants used were hybrids between high leptine producing selections of Solanum chacoense Bitt. and anther culture responsive selections of Solanum phureja Juz. et Buk. Several steps of the anther culture process were …
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Derivation and Comparison of Androgenic and Gynogenic Monoploid Potato Families
… were to derive androgenic and gynogenic monoploid populations from each of two diploid (2n = 2x = 24) Solanum phureja clones and compare their phenotypic characteristics in a greenhouse study. A haploid-inducing pollinator, S. phureja IVP101, was crossed to two selections of S. phureja …
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Use of monoploid solanum phureja in cell and tissue culture techniques for potato improvement
Monoploid genotypes (2n = x = 12), derived by anther culture of a diplandrous genotype of Solanum phureja, a South-American diploid potato species, were examined for their utility in germplasm development. Nine monoploid genotypes and the diploid anther-donor plant were grown in photoperiod …
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Analysis of anther-derived plants of Solanum phureja: variation in ploidy, photosynthetic efficiency and structure of the nuclear genome
… number of the S. phureja clones to the monoploid level and subsequently fusing genomes of two unrelated monoploid plants either by somatic hybridization or by cross-pollination between fertile doubled monoploids. Within this framework, the objectives of this research were to analyze …
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Protoplast Fusion for the Production of Intermonoploid Somatic Hybrids in Cultivated Potato
Monoploid potato genotypes represent plant material that is free from the "genetic load" of lethal and severely deleterious alleles normally present in the highly heterozygous cultivated potato species. Field evaluations enabled the identification of agronomically superior monoploid potato …
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Application of molecular markers to characterize potato plants derived from anther culture and protoplast fusion
… to the dihaploid level (2n=2x=24) and then the monoploid (2n=1x-12), or reducing diploid potato spp. to the monoploid level is a way of developing pure homozygous lines by doubling the monoploid genome. Homozygous breeding lines are important since potato is a highly cross pollinated crop and …
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Embryogenic response of potato anther culture to colchicine
… 56 plants were regenerated of which 11% were monoploid. The second experiment using the same colchicine treatments was conducted with 54 hybrids derived by crossing six anther-derived doubled monoploids with three heterozygous pollinators. A mean of 1.78 embryos per anther was obtained; again, …
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Factors affecting variability in anther culture and in regeneration of androgenic embryos of Solanum phureja
… were performed on several anther-derived monoploids of S. phureja to examine the frequency of nuclei at the 1x, 2x, and 4x levels within and among clones. Significant variation was found among duplicate cultures of the individual clones, but this variation was small enough to allow the …
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Derivation, fertility and breeding value of doubled monoploids from the diploid potato species, Solanum phureja
Thirty-two monoploids (<i>2n = x = 12</i>) derived by anther culture of ten diploid clones of <i>Solanum phureja</i> were used to generate doubled monoploids through in vitro shoot regeneration. Doubled monoploids were compared to the anther donor and progenitor monoploids for morphological …
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Transposon Tagging in Strawberry and Potato and Characterization of Representative Strawberry Mutants
… successful sequencing of the homozygous doubled monoploid clone of potato provides good insight into the study of important genes in this species in improving the pest resistance and improving yield. One approach to characterize gene function in a model system is having large populations of T-DNA …
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Derivation of interspecific Solanum hybrid genotypes with resistance to Colorado potato beetle (Leptinotarsa decemlineata Say)
… and the regeneration potential of anther-derived monoploids was evaluated. In vivo evaluation of interspecific hybrids was also performed. Three hybrids were anther cultured to observe the effects of reduced nitrogen source on androgenesis. Anthers were distributed to five reduced nitrogen …
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Inheritance of protoplast culturability and improvement in pollen development by protoplast manipulation in solanum
… resynthesizing superior diploids from selected monoploids, and tetraploids from selected diploids and dihaploids. Successful application of somatic hybridization largely depends upon protoplast culturability and regenerability of a plant. The ability of callus formation and plant regeneration …
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Genetic analysis of androgenetic competence and plants regenerated from callus culture of diploid potato species
… species. The transfer of desirable traits to a monoploid background can be expected using an- drogenetically competent selections in hybrid combination with germplasm expressing the desired attributes. In an attempt to determine genetic changes of regenerated potato plants following anther and …
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Development of intermonoploid somatic hybrids of potato and their molecular analysis based on polymorphism for retroelement Tst1
… cross-pollinating species. Four families of monoploids (2n=1x=12), developed from diploid hybrids with diverse genomic constitutions but heavily favoring Solanum phureja, a primitive cultivated potato, were used in electrofusion experiments to create intermonoploid somatic hybrids (SH). The …