Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 106 for “"monooxygenase"”.
-
Structural and functional models for methane monooxygenase
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1991.
-
Mechanistic Studies of Peptidylglycine Alpha-Amidating Monooxygenase (PAM)
… C-terminus. Peptidylglycine alpha-amidating monooxygenase (PAM) is a bi-functional enzyme which catalyzes the activation of peptide pro-hormones. PAM also functionalizes long chain N-acylglycines suggesting a potential role in signaling as their respective fatty acid amides. As chain length …
-
Structural enzymology of soluble methane monooxygenase protein-protein interactions.
Soluble methane monooxygenase (sMMO) is a multicomponent metalloenzyme that activates molecular oxygen, breaks the 105 kcal/mol C-H bond of CH4, and inserts one atom of O to create methanol at ambient temperature and pressure. This feat of catalytic prowess requires all three protein components of …
-
Mutagenic studies into the catalytic versatility of soluble methane monooxygenase
Soluble methane monooxygenase (sMMO) is a multicomponent bacterial enzyme that catalyzes the oxidation of methane to methanol, as well as oxidizing many other adventitious substrates. A number of mutagenic studies were carried out on the sMMO enzyme of Methylosinus trichosporium OB3b in order to …
-
Ammonia-monooxygenase-mediated cometabolic biotransformation and abiotic transformation of micropollutants
… were proposed based on MS2 spectra. Ammonia monooxygenase (AMO) was the responsible enzyme, given the competitive inhibition of asulam on AMO, and the instantaneous cease of asulam biotransformation when AMO was inhibited by octyne. More interestingly, hydroxylamine, the product of ammonia …
-
Steady State and Theoretical Investigations of Peptidylglycine α-Amidating Monooxygenase (PAM)
… to survival. Peptidylglycine alpha-amidating monooxygenase (PAM) is a bi-functional enzyme which catalyzes the conversion of peptide pro-hormones to peptide hormones. PAM is the only known mammalian enzyme that catalyzes the necessary alpha-amidation to activate these peptide hormones. PAM has …
-
Mechanistic and physical studies of methane monooxygenase from Methylococcus capsulatus (Bath)
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1995.
-
Circadian regulation and phytohormone responses of cytochrome P450 monooxygenase genes in Arabidopsis
… transcripts of cytochrome P450 monooxygenases (P450s) involved in their syntheses and catabolisms suggest an interactions between hormone signaling network and circadian signaling network. The first phase of my research profiled a total of 98 P450 genes in Arabidopsis thaliana as …
-
Structural studies of the hydroxylase component of methane monooxygenase from methyloccous capsulatus
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1994.
-
Structural investigations of hydroxylase proteins and complexes in bacterial multicomponent monooxygenase systems
… monooxgenases (BMMs) such as toluene/o-xylene monooxygenase (ToMO), phenol hydroxylase (PH), and soluble methane monooxygenase (sMMO) catalyze hydrocarbon oxidation reactions at a carboxylatebridged non-heme diiron center common to many systems in biology, as discussed in the first and …
-
Understanding orchestrated chemical reactions in toluene/o-xylene monooxygenase from pseudomonas sporium OX1
… in the family of bacterial multicomponent monooxygenases (BMMs), soluble methane monooxygenase (sMMO) and toluene/o-xylene monooxygenase (ToMO), are described. The functions and structures of the three or four components of sMMO and ToMO are summarized. Distinctly different dioxygen …
-
Structural and mutagenesis studies of soluble methane monooxygenase reductase from Methylococcus capsulatus (Bath)
… 27 kDa flavin binding domain of soluble methane monooxygenase reductase from Methylococcus capsulatus (Bath) was solved by NMR spectroscopy. The structure consists of a two domains, an FAD binding domain with a six-stranded antiparallel β-barrel and one α-helix, and an NADH binding domain with a …
-
Studies of the soluble methane monooxygenase : heterologous expression and reactions with nitric oxide
Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1999.
-
Exploring reactivity and component interactions in Toluene/o-Xylene Monooxygenase from pseudomonas sp. OX1
… and Four-Component Bacterial Multicomponent Monooxygenases. Bacterial multicomponent monooxygenases (BMMs) catalyze oxidation of hydrocarbon substrates through activation of dioxygen. Each BMM utilizes a diiron active site housed within a catalytic hydroxylase protein. This diiron active site …
-
Electron transfer and protein engineering studies of the soluble methane monooxygenase from Methylococcus capsulatus (Bath)
… of the Reductase Component of Soluble Methane Monooxygenase from Methylococcus capsulatus (Bath) ... Chapter 3. Expression and Characterization of Ferredoxin and Flavin Adenine Dinucleotide-Binding Domains of the Reductase Component of Soluble Methane Monooxygenase from Methylococcus capsulatus …
-
Purification and characterization of the hepatic microsomal monooxygenase system from the coastal marine fish Stenotomus chrysops
… cytochrome P-450 forms in trout and scup. Monooxygenase reconstitution experiments indicated that purified scup cytochrome P-450A actively hydroxylated testosterone at the 6ß position (turnover number = 0.8 nmol/min/nmol cytochrome P-450). Reconstituted cytochrome P-450B oxidized …
-
Heterologous production of recombinant peptidylglycine α-amidating monooxygenase for the production of biosimilar α-amidated peptides
… biological activity. Peptidylglycine α-amidating monooxygenase (PAM) is the only enzyme that catalyses the C-terminal amidation of peptides in vivo through its two catalytic cores, peptidylglycine α-hydroxylating monooxygenase (PHM) and peptidylglycine αamidating lyase (PAL). The cost and limited …
-
Bioinorganic hydrocarbon oxidation : mechanistic and kinetic studies of the soluble methane monooxygenase from Methylococcus capsulates (bath)
… as Exemplified by the Soluble Methane Monooxygenase -- Chapter 2. Small Molecule Binding to the Mixed-Valent Diiron Center of Methane Monooxygenase Hydroxylase from Methylococcus capsulatus (Bath) as Revealed by ENDOR Spectroscopy -- Chapter 3. An EPR Study of the Dinuclear Iron Site in …
-
Biochemical Characterization of the Two-component Monooxygenase System; Isobutylamine N-hydroxylase (IBAH) and Flavin Reductase (FRED)
… are part of a two-component flavin-dependent monooxygenase enzyme system that catalyze the conversion of isobutylamine (IBA) to isobutylhydroxylamine (IBHA), a key step in the formation of valanimycin, an azoxy antibiotic. In this work, we present the over-expression, purification and …
Page 1 of 6