Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 4 of 4 for “"mononuclear iron enzymes"”.
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Structural analysis of hydroxypropylphosphonic acid epoxidase : a fosfomycin biosynthetic enzyme
… is presented. Structural analysis of this cupin mononuclear iron enzyme in complex with its in vivo substrate, and comparison with apo- and holo-enzyme structures, provides insight into the mechanism of fosfomycin biosynthesis. In vivo, the enzyme catalyzes the biosynthesis of the epoxide …
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Superoxide stress in Escherichia coli
… new class of cellular targets of O2-: non-redox mononuclear iron enzymes. These enzymes employ a ferrous iron atom [Fe(II)] as a solvent-exposed cofactor. Four such enzymes -- threonine dehydrogenase (Tdh), ribulose-5-phosphate 3-epimerase (Rpe), peptide deformylase (PDF), and DAHP synthase -- …
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Secondary problems caused by the oxyr response to hydrogen peroxide stress in Escherichia coli
… (H2O2) diffuses into cells, where it reacts with iron via Fenton chemistry. Three cellular targets are damaged: DNA, mononuclear iron enzymes, and [4Fe-4S] cluster enzymes. E. coli uses the transcription factor OxyR to sense and respond to H2O2 stress. When the intracellular H2O2 levels rise to …
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Identification and characterization of a novel class of hydrogen peroxide targets in escherichia coli
… In this study, a novel class of H2O2-sensitive enzymes is described. I discovered that Hpx- cells were unable to metabolize gluconate via the pentose phosphate pathway. By assaying activity of various enzymes in the pathway, one protein, ribulose 5-phosphate 3-epimerase (Rpe), which requires a …