Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 227 for “"mononuclear cells"”.
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Endotoxin-induced microRNA expression in equine peripheral blood mononuclear cells
… expression of miRNAs in equine peripheral blood mononuclear cells (PBMCs). PBMCs were isolated from healthy adult horses and cultured with LPS or medium only for 2, 4 and 8 hours. Concentrations of inflammatory cytokines were measured in supernatants by immunoassay. Illumina Next-Generation …
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Peripheral Blood Mononuclear Cells Cytokine Expression in Horses Treated with Dexamethasone
… injection was administered. Peripheral-blood mononuclear cells were isolated from the blood samples and stimulated with concavalin A. RNA was isolated using the QIAGEN RNeasy kit. cDNA first strand synthesis was achieved using QIAGEN's OMMISCRIPT RT KIT. cDNA was also constructed for the house …
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Cytokine Gene Expressions of Peripheral Blood Mononuclear Cells in Acute Renal Allograft Rejection
… of mRNA extracted from peripheral blood mononuclear cells (PBMC) in post-transplant period. And, the patterns of gene expression of recipients who underwent rejection episode were compared with those of recipients who had no graft dysfunction. METHODS Among renal transplant recipient who …
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Peripheral blood mononuclear cells as non-invasive diagnostic indicators of stress-associated neural states
Researchers have demonstrated the ability to predict psychopathological states from human peripheral immune tissure transcriptional profiles, using microarrays. Although evidence in support of such an approach as a viable diagnostic avenue within psychiatric settings is accumulating, it remains to …
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Induction and characterization of endotoxin tolerance in equine peripheral blood mononuclear cells in vitro
… for inducing ET in equine peripheral blood mononuclear cells (PBMCs) in vitro, and to describe the cytokine profile which is associated with the ET. Blood was collected from 6 healthy horses and PBMCs were isolated. ET was induced by culturing cells with three concentrations of endotoxin …
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The early time course of autophagy in human peripheral blood mononuclear cells following endurance exercise
Exercise disrupts homeostasis and leads to the induction of an important catabolic system called autophagy. Autophagy is a beneficial cell survival process that is induced in periods of starvation. The purposes of this study are to (1) determine the time course of autophagy activation following …
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African swine fever virus CD2v protein induces β-interferon expression and apoptosis in swine peripheral blood mononuclear cells
Embargo set by: Seth Robbins for item 121229 Lift date: 2024-01-12T22:55:09Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system
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Development and characterization of recombinant human autoantibody using cDNA made from peripheral blood mononuclear cells of patients with bronchial asthma
… total RNA 추출 3 1. 말초혈액 단핵구 세포 (peripheral blood mononuclear cell, PBMC) 분리 3 2. Total RNA 추출 4 3. mRNA 분리 4 C. Polymerase chain reaction (PCR)을 이용한 cytokeratin 18 (CK-18) 단백질에 대한 인간 single-chain variable fragment (scFv) 항체 라이브러리의 구축 5 1. cDNA 합성과 중합효소 연쇄반응 (PCR) 5 2. scFv의 heavy chain과 light …
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The influence of equine bone marrow derived stem cells on the response of cultured peripheral blood mononuclear cells to endotoxin
… supportive care at this time. Mesenchymal stem cells (MSCs) have been shown to have anti-inflamamtory and immune modulatory effects that may have some benefit for the treatment of horses with endotoxemia. To evaluate the effect of equine MSCs on the response to endotoxin challenge, the study was …
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Analysis of the Immunomodulatory Properties of Mycobacterium Cell Wall Fraction on the Cytokine Production of Peripheral Blood Mononuclear Cells of Healthy Dogs
… induced by MCWF in canine peripheral blood mononuclear cells (PBMCs), using enzyme-linked immunosorbent assays (ELISA) and quantitative reverse-transcription polymerase chain reaction (qRT-PCR) for quantifying cytokine protein and mRNA transcripts, respectively. PBMCs were isolated from …
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Involvement of the LFA-1 ligand JAM-A in inflammatory recruitment of mononuclear cells : identification of the JAM-A binding domain in LFA-1
… of JAM-A in inflammatory recruitment of mononuclear cells was analyzed. Furthermore, a functional contribution of JAM-A to atherogenesis was investigated. By deletion of the I domain in the aL subunit of LFA-1 and expression of this aL mutant in aL-deficient Jurkat J-ß2.7 T cells, it was …
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Redefining endothelial progenitor cells using a proteomics approach
The concept of endothelial progenitor cells (EPCs) has attracted considerable interest in cardiovascular research, but despite a decade of research there are still no specific markers for EPCs and results from clinical trials remain controversial. To identify membrane proteins, which could serve as …
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The Satellite Cell of Skeletal Muscle
… be readily distinguished from other better-known mononuclear cells found lying between muscle fibres, such as endomycial fibroblasts, vascular pericytes, mast cells and histiocytes. It is unique in that it is the only mononucleated cell lying within the basement membrane of the normal adult muscle …
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A role of statins against listeria monocytogenes and Mycobacterium tuberculosis infection
… decreased growth of Mtb in peripheral blood mononuclear cells (PBMC) and monocyte†derived macrophages (MDM) isolated from patients with familial hypercholesterolemia (FH) on statin therapy when compared to healthy subjects. Together, our results show that statins induces protection against …
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Terapia celular na pseudo-artrose
… of the bone marrow aspirate was compared to mononuclear cells purified extemnporaneously using the Sepax® equipment. Seven patients with nonunion of the tibia or femur were treated. Five received a percutaneous infusion of autologous bone marrow aspirated from the iliac crest, and two …
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Active Inhibition of T Cell Proliferation by Monocytes
… in activation and inhibition of peripheral blood mononuclear cells. In this study, it is shown that the inhibition of OKT3-dependent T cell activation correlates with metabolic activation of antigen presenting cells. In contrast, inhibition of lectin- and superantigen-mediated T cell activation …
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Cryopreservation of pancreatic islets
… complex architecture of pancreatic islets, human mononuclear cells were first used to optimise the use of permeabilising polymers. Current methods of viability assessment were critically examined and improved, followed by systematic screening of cryopreservation conditions. The efficacy of …
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