Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 44 for “"monoclonal antibody (Mab)"”.
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Monoclonal antibody (mAb) purification by counter current chromatography (CCC)
… extended to the rapid, scalable purification of monoclonal antibodies (mAb) from mammalian cell culture, using aqueous two-phase systems (ATPS) of inorganic salts and polymer. A polyethylene glycol (PEG) and sodium citrate system was found to be the most appropriate by robotic phase system …
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Continuous Antibody Titer Assessment of the Biomanufacturing Process using Nanofluidic Binding Assays
Online monitoring of monoclonal antibody (mAb) product titers throughout biologics process development and production enables the implementation of quality-by-design biomanufacturing, rapid bioprocess decision-making, and process optimization. Intermittent sampling and analysis are presently …
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Correlated long-distance dynamics modulate monoclonal antibody binding resistance in flaviviral envelope pretein domain-3: A molecular dynamics simulations study
Numerous monoclonal antibody (MAb) binding resistant mutations have been localized to the envelope protein domain-3 (ED3) of flaviviruses. Previously it was shown that regions constituting antibody binding sites of dengue-2 (DEN2) and West Nile virus (WNV) ED3 were energetically coupled with the …
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Surface Directed Monoclonal Antibodies against STEC Aid in the Reduction of Pathogen Detection Times from Food and Water
… pore sizes), followed by capture using specific monoclonal antibody (mAb)-coated Dynabeads, and detection via fluorescence microscopy, (2) the addition of enrichment culture into a flow through system consisting of a column packed with large polystyrene beads (≥ 100 μm) coated with specific mAbs …
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Epiregulin as a Novel Target for Antibody-Drug Conjugate Development for the Treatment of Colorectal Cancer
<p>Antibody-drug conjugates (ADCs) are a fast-growing class of molecularly targeted therapies with 13 currently approved and over a 100 in clinical trials. ADCs consist of a monoclonal antibody (mAb) conjugated to a potent cytotoxic payload to selectively target tumor antigens highly expressed on …
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The Cardiovascular Effect of GLP-1 in Humans
… with an extensively validated monoclonal antibody (mAb). There was no effect of GLP-1 on forearm blood flow, nor were systemic hemodynamics or cardiac output affected, and no binding of GLP-1R mAb was detected in vascular tissue. GLP-1 reduced resting coronary transit time (Tmn …
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Evaluation of a Monoclonal-based EIA for the Detection of Giardia lamblia and the Identification of the Antigen
… In the first phase of this study, I developed a monoclonal-based EIA, the GIARDIA TEST, with these qualities in mind. This assay's performance characteristics were determined by a comparison study using conventional ova and parasite examination, immunofluorescence antibody test (IFA) and other …
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Intravital Imaging of Dynamic Behaviors of Leukocytes in UVB-induced Skin Inflammation
… treatment. Depletion of neutrophils by anti-Ly6G monoclonal antibody (mAb) administration did not affect LCs migration in response to the same UVB radiation, but rather promoted LC precursors’ repopulation to the epidermis after UVB treatment. In addition, depletion of neutrophils greatly …
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Application of Anti-H Monoclonal Antibodies to Develop Rapid Immunoassay for Vibrio vulnificus
… and rapid. Application of species-specific monoclonal antibody (MAB) can increase the sensitivity and speed of V. vulnificus detection by eliminating enrichment steps, hence the objective of our study was to develop antibody based rapid and sensitive V .vulnificus detection methods. In the …
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Antibody engineering for cancer therapy
… cancer. In this Thesis, novel antibodies and antibody-conjugate against two tumor antigens, AF-20 antigen and human aspartyl (asparaginyl) [beta]- hydroxylase (HAAH), were developed. Previously, these two tumor antigens have been shown to be present on a variety of tumor cells, while they have …
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Modeling and economic evaluation of early stage clinical monoclonal antibody manufacturing using single use technology
… investigates the current state of clinical monoclonal antibody (mAb) manufacturing and generates possible future states for low-cost, flexible manufacturing. Pfizer currently has a world-class clinical manufacturing facility in St. Louis, MO, and forecasts have not predicted sufficient …
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Separação imunomagnética de cistos teciduais e esporocistos de toxoplasma gondii pelo anticorpo monoclonal K8/15-15 e caracterização de sua proteína alvo por Western Blot e Imunoblot
… and to isolate the protein recognized by the monoclonal antibody (mAb) K8/15-15. This was the first described mAb that is able to simultaneously recognize proteins in tissue cyst and sporocyst walls of the parasite. Using IMS, more than 2,000 in vitro produced tissue cysts were isolated from a …
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Understanding the Mechanism of Action of Rituximab in the Reversal of Multidrug Resistance in a Non-Hodgkins Lymphoma Cell Line
… been previously demonstrated that an anti-CD20 monoclonal antibody (MAb) can reverse MDR in B lymphoma cells in vitro. However, the mechanisms underlying this effect are unknown. A recent study showed that anti-CD20 MAbs could induce rapid redistribution of CD20 into a detergent-insoluble …
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Transdermal delivery of heavy chain only antibody binding domains using hydrogel-forming microarray patches
Since the licensing of the first monoclonal antibody (mAb) over 30 years ago, the mAb industry has expanded exponentially, with six of the top 10 blockbuster drugs in 2018 being antibody-based. However, rather than resting on their laurels, drug developers have continued to innovate. This has led …
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Alternate pathways of cytotoxic T lymphocyte and natural killer cell activation
… In the present study, we demonstrate that monoclonal antibody (mAb) 9F3, directed against murine CD44 expressed on cytotoxic T lymphocytes (CTls), can trigger the lytic activity of CTls and redirect CTl-mediated lysis to antigen-negative Fc receptor-positive target cells. Similar redirected …
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Cyclic AMP and the Induction of Reactive Astrogliosis
… to a reactive-like state. Studies have shown monoclonal antibody (mAB) Jl-31 may recognize a phosphoepitope found on the nuclear intermediate filament, lamin, as well as the cytoplasmic intermediate filament characteristic to mammalian astrocytes, glial fibrillary acidic protein (GF AP), …
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Characterization of epitope-blocking ELISA for differential diagnoses of secondary flavivirus infections
… project investigated the ability of WNV-specific monoclonal antibodies to compete actively in binding the epitopes on the NS1 peptides and to distinguish between antibodies induced by different flaviviruses. Developing a test distinguish between antibodies to different flaviviruses would …
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Role of CD4+CD25+ Regulatory T Lymphocytes in Experimental Toxoplasmosis
… cells was accomplished by injecting mice with a monoclonal antibody (Mab) isolated from the 7D4 rat hybridoma cell line. This Mab is specific for the interleukin-2 receptor chain (also known as CD25). Female Swiss Webster mice of approximately 6-7 weeks of age were depleted of Treg cells by …
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Retroviral-mediated gene transfer of Il-1β?cDNA into human activated T cells
… in human T cells using 1) immobilized anti-CD3 monoclonal antibody (mAb) plus interleukin-2 (IL-2), or 2) cis costimulation using beads carrying co-immobilized anti-CD3 and anti-CD28 mAbs. By cross-linking the CD3 and CD28 receptors, these mAbs mimic in vivo signaling events, leading to cytokine …
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