Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 23 for “"molecular typing"”.
-
Molecular typing of virulence genes in enterotoxigenic Bacillus cereus
Bacillus cereus causes emesis and/or diarrhea following ingestion of contaminated food due to the production of emetic toxins and enterotoxins. SYBR Green I is used as an intercalating dye and its florescence increases as a result of DNA amplification during real-time PCR. A second-derivative plot …
-
Molecular Typing Of Mycobacterial Isolates Cultured From The Tissue Of Inflammatory Bowel Disease (Crohn's Disease) Patients
… this two year pilot study, an array of three PCR molecular assays based on the detection of sequences from the16S rRNA, IS1245, and IS900 genes, belonging to members of the MAC, have been developed and optimized into a common protocol to be used as a rapid and accurate diagnostic tool regarding M. …
-
Pandemic <em>Vibrio parahaemolyticus</em>: Defining Strains Using Molecular Typing and a Growth Advantage at Lower Temperatures
… has been discovered. This study investigated the molecular characteristics of the pandemic strain and growth characteristics at different temperatures. The hypothesis tested was that pandemic strains of V. parahaemolyticus have modifications to their proteome that give a selective advantage over …
-
Epidemiology of Pseudomomas aeruginosa and Achromobacter xylosoxidans in Belgian cystic fibrosis patients, relying on molecular typing techniques
Pseudomonas aeruginosa is known to be the most important pathogen in CF, and is associated with increased morbidity and reduced life expectancy. Although we are convinced that peer contacts are psychologically beneficial for patients dealing with CF, we wanted to ensure that our patients did not …
-
Characterising variation in the gene for the Dichelobacter nodosus Type IV fimbrial protein: Development of a molecular typing system
… of footrot vaccines. AfimA based PCR-RFLP typing system was described and potentially represents a quick and cost effective means of typing D.nodosus from lesion material. The development of molecular techniques to rapidly "type" D.nodosus may enable strain-specific vaccines to be …
-
Epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) in a university medical center day care facility
… Isolates were analyzed for relatedness using molecular typing. Statistical analysis was performed. \r\n\r\nRESULTS: The prevalence of MRSA in the children was 6.73%. One employee (3.13%) was colonized with MRSA. Cultures of 6 of 17 (35.3%) family members of participants positive for MRSA …
-
Characterisation of propionibacterium acnes
… virulence factor expression and serotype. A molecular typing method for P.acnes was developed using random amplification of polymorphic DNA (RAPD). Patient serum was used to screen P.acnes strains for antigens expressed in vivo and the chemical composition determined. The serodiagnostic …
-
A Genotypic Comparison of Staphylococcus lugdunensis Clinical Isolates of Diverse Geographic Origin
… various geographic regions was assessed using molecular typing methods, specifically, pulsed-field gel electrophoresis (PFGE), arbitrarily primed polymerase chain reaction (AP-PCR), inter repetitive sequence polymerase chain reaction (REP-PCR), and southern hybridization.|PFGE together with …
-
Detection and characterisation of carbapenem-resistant gram-negative bacilli infections in Ghana
… activity by using simple phenotypic tests, molecular typing to characterise the resistance genes and to determine the relatedness of the CR isolates collected from different hospitals in the country. A total of 111 CR isolates were identified by disc diffusion susceptibility testing and the …
-
Mycobacterium tuberculosis strains in New Zealand: phylogeny and structural biology
… Pacific People are disproportionally affected. Molecular typing shows that approximately two-thirds of M. tuberculosis isolates from New Zealand-born patients can be assigned to clusters of related strains. The largest M. tuberculosis cluster in New Zealand is known as the ‘Rangipo’ cluster and …
-
Arthropod-borne infections in the United Kingdom and Saudi Arabia
… from these different hosts using PCR- based molecular typing tools. Trypanosoma infections were found in mall rodents from the UK and Ireland, however the presence of the invading bank vole (Myodes glareolus) in Ireland appears to have disrupted the host-parasite relationship between wood …
-
Exploring the Evolution of Pathogenicity in a Mult-host Bacterial Pathogen Using Comparative Genomics
… and genomic concepts. Chapter 2 describes molecular typing of clinical Pantoea isolates obtained from hospital patients. Of 54 clinical isolates, 41 were confirmed as Pantoea, with the majority of these isolates found to belong to P. septica. Chapter 3 involves verification of the taxonomy …
-
Bats and their endoparasites : characterising pipistrelle infections and toll-like receptor (TLR2 and TLR4) gene variations
… population through development of PCR-based molecular typing tools. This approach has allowed the molecular differentiation between Trypanosoma dionisii and T. vespertilionis infections, confirmed that all eimerian infections were caused by Eimeria rioarribaensis and also confirmed that …
-
Seroprevalence and characterization of Brucella SPP. isolated from humans and cattle in Southern and Western provinces of Zambia..
… human blood samples, were cultured and analysed. Molecular analysis (16S rRNA PCR, Real-Time PCR, Multiplex Bruce Ladder PCR) was conducted to isolate, identify and characterize Brucella spp. The Brucella isolates were evaluated for susceptibility to six antimicrobials using the disk diffusion …
-
Seroprevalence and characterization of Brucella SPP. isolated from humans and cattle in Southern and Western provinces of Zambia..
… human blood samples, were cultured and analysed. Molecular analysis (16S rRNA PCR, Real-Time PCR, Multiplex Bruce Ladder PCR) was conducted to isolate, identify and characterize Brucella spp. The Brucella isolates were evaluated for susceptibility to six antimicrobials using the disk diffusion …
-
Phenotypic and genotypic characterization of salmonella isolates from chickens and humans in South Sudan.
… characterization was done through serology, biotyping and antimicrobial susceptibility profiles while the genetic diversity was determined through molecular typing methods that included the detection of virulence genes by PCR and whole genome sequencing of targeted genes. The data was analyzed …
Page 1 of 2