Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 118 for “"molecular tools"”.
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Optimization of molecular tools for high-throughput genetic screening
… agreed on, the efficacy of currently available tools limits the strength of conclusions drawn from these experiments. This thesis describes a method to optimize molecular tools for high-throughput screening, both for shRNA and sgRNA based reagents. Using large shRNA efficacy datasets, we first …
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DEVELOPING MOLECULAR TOOLS TO IMPROVE TERPENOIDS PRODUCTION IN RHODOSPORIDIUM TORULODIES
Terpenoids produced are usually highly toxic to the host cells, leading to early shut-off of the biosynthesis. Red yeasts, particularly species in Rhodosporidium, were considered high-yielding platform for terpenoids production due to their remarkable potentials for lipid production and their …
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Development of novel light-based molecular tools for biomedical applications
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2026-08-01
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Design and synthesis of molecular tools for the ubiquitin proteasome system
Alzheimer's disease (AD) has become one of the biggest global public health challenges, with 0.5% of the population living with dementia. The ubiquitin proteasome system (UPS), in particular the interaction between a mutant form of ubiquitin, Ubb+1, and the E2-conjugating enzyme E2-25K, has been …
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Development of activatable molecular tools for applications in light-mediated cancer therapy
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2027-08-01
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Expanding the molecular tools for the microalgae Chlorella vulgaris and Phaeodactylum tricornutum
… through engineering. This study focussed on molecular tool development to address bottlenecks hindering the engineering of two microalgal species: the green alga *Chlorella vulgaris* and the marine diatom *Phaeodactylum tricornutum*, both exhibit rapid growth rates and high density cultures …
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Shedding Light on Decay Kinetics of Critical Wastewater Bacteria with Molecular Tools
… is challenging to assay. Recently, more specific molecular techniques have been developed, such as DNA and RNA extraction with qPCR and RT-qPCR, ATP assays and live/dead cell-staining. However, these assays have not been widely accepted or bench-marked against traditional techniques. It is …
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Micro electrochemical sensors and PCR systems: cellular and molecular tools for wine yeast analysis
… for reliable, sensitive and low-cost analysis tools. Small reagents volumes, low power consumption, portability, fast analysis, high throughput and systems integration are the key aspects that make these systems more and more appealing within both the academic and industrial communities. In the …
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Development, Characterization, and Use of Molecular Tools to Study Immune-Driven Zika Virus Evolution
… highlighting novel technologies to facilitate molecular virology and demonstrating that cross-reactive immune selection has the potential to alter the evolutionary trajectory of flaviviruses. This work provides critical information to understand how flaviviruses are evolving and emerging, and …
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Development and utilization of molecular tools to understand invasion biology in Centaurea maculosa (spotted knapweed)
… University was designed to create and utilize molecular tools to help understand ecological phenomena in the invasive weed, spotted knapweed (Centaurea maculosa Lam.). In this dissertation, I first introduce the need for research at multiple scales and the potential benefits of collectively …
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Understanding the Influence of Banded Mongoose (Mungos mungo) Social Structuring on Disease Transmission Using Molecular Tools
Understanding the disease transmission dynamics in wildlife species can be difficult and can prove more complicated if the population structure of a socially living species is shaped by territoriality. Understanding the connections and movements of individuals between groups is vital to documenting …
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Use of molecular tools to investigate the prevalence and transmission routes of Toxoplasma in woodmice and humans
… populations. The aims of this studywere to use molecular biological methods to investigate parasite transmission in wildrodents and humans.In this study, the prevalence of Toxoplasma was determined in a natural population ofwoodmice (Apodemus sylvaticus) which probably have infrequent …
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Development of molecular tools for rapid detection and quantification of indoor airborne molds to assess their impact on public health
… have an impact on human health. In this context, molecular tools seem to be a valuable alternative. In this PhD work, different molecular tools were developed, from simplex to multiplex, to detect and identify indoor airborne fungi. The goal was to improve the detection of fungal contaminants, …
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Whole genome analysis of the koa wilt pathogen (Fusarium oxysporum f. sp. koae) and development of molecular tools for early detection and monitoring
Pathogenic and non-pathogenic Fusarium oxysporum are morphologically indistinguishable from each other. Pathogenic F. oxysporum f. sp. koae (Fo koae) is a limiting factor for low to mid elevation, below 610m (2000 ft), Acacia koa forests and timber stands. These warmer, lower elevation sites are …
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Evaluation of Nitrogenase Genes as Markers in Soil Microbial Community Studies
Molecular tools were developed to overcome some of the constraints to analyzing nitrogen-fixing bacteria in the environment. Two distinct plant-microbe systems were used to develop these tools, and to evaluate their utility and flexibility. The first system focused on nitrogen-fixing bacteria in …
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From metacommunity dynamics to rapid biodiversity assessment: DNA-based approaches expand horizons in both fundamental and applied ecology
Molecular tools have long been recognised as having enormous potential to expand horizons in ecology, but the promise remains substantially unfulfilled. In this thesis, I apply genetic approaches to two ecological problems that have proved difficult to solve using traditional techniques. Chapters 1 …
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Laying the Genetic and Molecular Foundation for the Study of Fusobacterium Nucleatum in Relation to Human Health and Disease
… disease, remarkably little is known about the molecular mechanisms underlying these infections. This knowledge gap can, in part, be attributed to a lack of molecular tools and experimental workflows. Creating the genetic tools to fill this knowledge gap is an imperative undertaking for the …
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