Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 49 for “"molecular detection"”.
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Molecular detection of melanoma nodal metastases
… on achieving maximum specificity for the detection of melanoma nodal metastases. A novel protocol for the efficient homogenisation of nodal tissue was developed, with clinical applicability as the objective.
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Molecular Detection for the Apicomplexan Parasites Cyclospora cayetanensis and Cryptosporidium spp.
… settings. Establishing affordable molecular detection techniques will allow for the reliable determination of these parasites’ prevalence in those settings. Objective: The overarching objective was to optimize polymerase chain reaction (PCR) protocols to detect the presence of C. …
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Molecular detection and characterization of Bartonella in small mammals from southern Africa
… Gauteng and Western Cape) in South Africa. Molecular estimates of Bartonella infection rates were determined through multi-gene screening of DNA extracted from clinical samples, primarily heart and spleen. PCR assays targeting the citrate synthase (gltA) and NADH dehydrogenase gamma subunit …
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Molecular detection and charcaterisation of biologically relevant free radicals during surgical ischaemia-reperfusion
… and with experimental EPR values. Calculated molecular structures, EPR (electron paramagnetic resonance spectroscopy), the vibration spectral and energetic properties and all are reported including some proposed changes to previous EPR assignments. Conformer 2 of L-ascorbic acid is predicted …
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Molecular detection of the apicomplexan parasite 'X' (APX) in bivalves in New Zealand
… taxonomic identity. There are also no specific detection or control measures available to deal with disease outbreaks. Therefore, the aim of this research was to begin to fill the gaps in our knowledge of APX to assist in understanding and combating the disease. Two unique APX 18S rRNA gene …
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Molecular Detection and Characterization of Rickettsia sp. in Hard Ticks Collected in Nacogdoches, Texas
… These methods include surveillance of ticks and detection of the tick-borne pathogens.This study examined hard ticks in Nacogdoches, Texas, to test for the presence of <em>Rickettsia </em>sp. 47 ticks were collected from vegetation by flagging and their species, sex, and developmental stage were …
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Soft rot Enterobacteriaceae-molecular detection and biocontrol under in vitro and in vivo conditions
… of bacteria isolated from carrots led to the detection of P. carotovorum and Dickeya sp. This is the first study to provide knowledge about Pectobacterium and Dickeya spp. diversity causing soft rot of vegetables in Northern Ireland confirmed by real-time PCR and DNA sequencing. <br/><br/>The …
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Molecular detection and identification of phytoplasmas in sugarcane in Hawaii, Thailand, Cuba and Near East
… our knowledge, this is the first report for the detection and identification of phytoplasma in sugarcane plants from Hawaii, Egypt and Syria. Our investigation on Thai sugarcane plants was in agreement with previous literature where sugarcane white leaf (SCWL) phytoplasma is associated with white …
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Molecular detection and factors associated with selected tickbone zoonoses in dogs in Chilanga district in Zambia
Vector borne diseases, also referred to as zoonoses, have been responsible for some of the worst plagues of mankind. The close relationship between humans and animals increases chances of transmission of vector-borne zoonoses. Among some of these infections are pathogens transmitted by ticks which …
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Molecular detection and factors associated with selected tickbone zoonoses in dogs in Chilanga district in Zambia
Vector borne diseases, also referred to as zoonoses, have been responsible for some of the worst plagues of mankind. The close relationship between humans and animals increases chances of transmission of vector-borne zoonoses. Among some of these infections are pathogens transmitted by ticks which …
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Molecular Detection of Tick-Borne Pathogens Associated With Ixodid Tick Species Infesting Migratory Songbirds and Ruminants
<p>Ticks are hematophagous arthropods and capable of inoculating various infectious agents to their vertebrate hosts. Ticks attached to birds and ruminants are capable of carrying tick-transmitted microorganisms. In our study, the prevalence of tick-borne pathogens was examined in ticks infesting …
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Rapid and portable molecular detection tools for reptile pest priority species in Australian environmental biosecurity scenarios
… (LAMP) as an emerging tool for the DNA detection of high-risk species in environmental biosecurity and compliance contexts. To help combat the trade of B. constrictor and P. guttatus I have developed fit for purpose LAMP assays for environmental biosecurity compliance. I rigorously …
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Enhanced Rainbow Trapping in Width-graded Chirped MIM Nano-gratings for High-sensitivity Multiwavelength Bio-molecular Detection
… enhancement factors of 106–107 are possible for detection of 1mM aqueous phospholipid solution at 532 nm, 638 nm, and 785 nm laser illumination. The highly intense multiwavelength characteristic of these nano-grating facilitates access to weak Raman modes. We employ MIM width-graded nano-gratings …
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Molecular detection and identification of Cryptosporidium species isolated from human and animal sources in Limpopo and Gauteng Provinces
… region; Real-time PCR employing SYBR Green detection format for amplification of 18S rRNA region; Real-time PCR employing Hydrolysis probes detection format for amplification of SSU rRNA region; Real-time PCR specific for amplification of C. hominis region and C. parvum region. Positive …
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Effects of Organic and Conventional Agricultural Practices on Soil Microbial Communities and Molecular Detection of Soil Borne Disease
… environment that individual farms create. Early detection of soil borne disease is essential for agricultural success. However, monitoring incidence of disease based on plant growth response to pathogenic inoculation may not reveal the amount of pathogenic DNA in soil. A comparative study of …
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Molecular detection, quantification and characterization of extended-spectrum β-lactamase (ESBL)- and AmpC-encoding genes in a cucumber agroecosystem
… transformation mediated by the plasmids. Herein, molecular methods such as endpoint PCR, Sanger sequencing, whole genome sequencing (WGS) and digital droplet PCR (ddPCR) were used to gain knowledge about AMR in agriculture. This knowledge will be useful in improving AMR surveillance and thus …
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Survival and recovery characteristics of Arcobacter butzleri in groundwater microcosms
… environmental sources comparing cultural and molecular methods. Cultural methods using selective media proved inferior to molecular methods. Molecular detection also had limitations, though. Selective enrichment and nucleic acid purification may resolve these limitations.
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Electrokinetic trapping of biomolecules : novel nanofluidic devices for proteomic applications
… nanofluidic filters. With the distinct ionic and molecular interaction at the nanoscale, nanofluidic systems can potentially outperform current sample preparation and molecular detection techniques. Aiming to investigate and expand the applications of these techniques, this thesis work involves …
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Deposition of electronic and plasmonic materials inside microstructured optical fibres
… suited for Surface Enhance Raman Scattering molecular detection.
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