Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 11 of 11 for “"molecular crowding"”.
-
Mechanisms of biochemical reactions within crowded environments
… tools to disentangle the effects of molecular crowding on biochemical processes. First, we propose a lattice model to study the implications of molecular crowding on enzymatic reactions. We provide a detailed picture of how crowding affects binding and unbinding events and how the …
-
Effects of Osmolytes on Amyloid-beta Protein Surface Activity and Membrane Interactions
… up to 40% of the cellular volume. The resulting molecular crowding and preferential exclusion modulate the thermodynamics of protein reactions to favor those that reduce total system volume and minimize solvent exposed surface area, respectively. To assess the effects of molecular crowding and …
-
Single-Molecule Studies Of Local And Global Nucleic-Acid Dynamics
… been invaluable in elucidation of various biomolecular mechanisms, hence contributing significantly to the understanding of biological events. However, a clear understanding of how molecules function in the cellular context requires investigation of their interconversion between multiple …
-
Study of the Dynamics of Retaining GT-B Fold Glycosyltransferases Using Hydrogen Deuterium Exchange Mass Spectrometry
… mechanism. The effect of molecular crowding on the catalysis of the retainingGT-B is explored, revealing varied effects. Not all crowding molecules uniformly influencecatalytic rates, with kinetic parameter analysis suggesting a minor role of volume exclusion.Conversely, …
-
HOW MICROVESICLES ARE "BUDDING" INTO THE CANCER CONVERSATION: THE ROLE OF GLYCOCALYX-INDUCED MEMBRANE BENDING
… and microvesicle biogenesis. We demonstrate that molecular crowding on the plasma membrane by flexible glycocalyx biopolymers allows membrane bending to occur. Moreover, in coordination with the actin cytoskeleton, glycocalyx biopolymers induce membrane bending required for generation of shapes, …
-
Phase separation in giant vesicles
… solution. This phenomenon might be related to molecular crowding, fractionation and protein sorting in cells. To elucidate this process we used two chemically dissimilar polymers, polyethylene glycol (PEG) and dextran, encapsulated in giant vesicles. The dynamics of the phase separation of this …
-
Structure and dynamics of plant cell walls and membrane peptides from solid-state NMR
… caused by site-specific interactions instead of molecular crowding. By measuring water to polysaccharide spin diffusion in intact and sequentially digested walls, we are able to examine the three-dimensional structure of cell walls. Our results suggest a single network model, where cellulose …
-
Macromolecular organization of flavonoid biosynthesis in Arabidopsis thaliana
… now known to exist as enzyme complexes due to molecular crowding effects in vivo. These differences have contributed to the controversy that surrounds explanations of how metabolic pathways are spatially organized and regulated in the living cell. The organization of enzymes in vivo is now …
-
Development of the single-molecule tracking and fluorescence lifetime techniques for biophysical measurements and biomedical applications
… acids without the need for synchronization of molecular states can be achieved with single-molecule detection, but the current experiments are not carried out in a native cellular context. In this dissertation, I developed an integrated 3D single-molecule tracking (3D-SMT) and lifetime …
-
Printing methodologies for functional bioarrays
… functional bioarrays in order to study intermolecular interactions in confined environments and microarray format. In particular, inkjet printing was employed for the generation of picoliter-scale aqueous droplets stabilized against evaporation and molecular leakage by oil-confinement with …
-
Microfluidic Approaches for Investigating Aggregated Forms of Disease-Related Proteins
… that the most toxic species are the low molecular weight oligomers formed during the onset of the aggregation process. These oligomeric species are commonly difficult to analyse due to their low abundance, transient nature and heterogeneity in their structure. In fact, prions, known to be …