Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"molecular biology methods"”.
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Structural Studies on the Regulation of Sporulation in Bacillus and Antibiotic Sulfation
… an uncultured soil bacterium. Using standard molecular biology methods and the techniques of X-ray crystallography, synthetic chemistry, and protein semisynthesis, we have: (1) Solved the X-ray crystal structure of a Geobacillus stearothermophilus sporulation histidine kinase in complex with …
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Antioxidant activity of Bulgarian medicinal plants and related biological effects // Антиоксидантна активност на български лечебни растения и свързани с нея биологични ефекти
… two approaches, and by the means of modern molecular biology methods: (1) In vitro to determine the antioxidant activity and the phytochemical composition; 2) In vivo studies in experimental animals and humans, and on cell cultures. Scientific evidence is obtained that the total polyphenol …
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Microbial characterisation of bioaerosols from indoor environments using molecular techniques.
… of the airborne microbial communities. Molecular biology methods demonstrate a great potential for improving our comprehension of the significance of the indoor aerosol microbial load in the context of human exposure, providing in-depth characterisation of microbial communities. However, …
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Exposure, health effects, immunological markers and biomarkers of susceptibility among Swedish rubber workers
… analysis. Genotypes were established using molecular biology methods. Compared to controls, exposed workers had in average 420% higher urinary TTCA level and 200% higher urinary 1-HP level. The levels of TTCA and 1-HP differed between different rubber companies and their subdivisions, as …
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Biological response to 8-oxoguanine base released during DNA base excision repair
… cell culture models and advanced cellular and molecular biology methods such as microarray, ROS detection assays, immunoblot, qRT-PCR, shRNA and siRNA technology, microscopy, as well as LC/IDMS for quantification of 8-oxo-Gua to test this hypothesis, we: 1) established that 8-oxo-Gua-induced …
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Cellular clocks on steroids: the mechanism of circadian entrainment by glucocorticoids
… In this thesis, I have used contemporary molecular biology methods to directly test the 20-year-old canonical model for GC-resetting and elucidate its mechanistic underpinnings. First, I established a robust cellular assay to characterise GC-resetting in multiple in vitro model systems, …
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Structural study of ExsA, the regulator of Type III Secretion System of Pseudomonas aeruginosa
… related infections. With a combination of molecular biology tools and structural biology methods, we solved the N-terminal domain structure of the ExsA protein in P. aeruginosa. The model of the ExsA N-terminal domain has enriched our knowledge about ExsA dimerization and can serve as the …
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The differential effects of short-term physical exercise, environmental enrichment and their combination, and short- and long-term environmental enrichment on behavior and associated molecular changes in the brain of C57BL/6 mice
… of EE, if any, on behavior and underlying molecular biology. Methods: We analyzed the differential effects of short-term PE, EE, and PE+EE, and the effects of short- vs. long-term EE on cognition-, anxiety- and depressive-like behaviors in 4-, 9- and 14-month-old C57BL/6 mice using an …
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CRISPR/CASTE: Functional Genomic Studies of the Major Evolutionary Innovations of Ants
… two particularly derived features of ant biology: complex chemical communication and caste plasticity. To study these features, I had a particular focus on generating and characterizing germ-line mutants. I believe that the study of mutants, and applying molecular biology methods more …
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The Neurotoxicity of Insecticides to Striatal Dopaminergic Pathway
… the C57BL/6 mouse model, using biochemical and molecular biology methods. In the first objective, I investigated the neurotoxicity in striatal dopaminergic pathways following co-application of permethrin (PM), chlorpyrifos (CPF) and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). The study …
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Identifizierung und Charakterisierung entwicklungsabhängig hochregulierter Gene im Innenohr der Ratte
Durch die außergewöhnlichen Sinnesleistungen benötigt das Säugetiergehör eine Vielzahl spezifischer Proteine. Bislang ist nur ein Teil dieser Proteine bekannt und in ihrer Funktion für das Innenohr beschrieben worden. <br>In dieser Arbeit wurden Gene identifiziert, die während der postnatalen …