Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"molecular beacons"”.
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A Novel Forensic DNA Profiling Method Based On Molecular Beacons Without Dna Purification
… the real-time monitoring of the interaction of molecular beacons (MBs) with the template. These MBs can then be used to identify the presence or absence of specific nucleotide polymorphisms. This increase in throughput has extensive application in forensic and medical applications. </p>
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Single-molecule detection of molecular beacons generated from LDR on thermoplastic microfluidic device for bioanalysis
… This research aims to develop an innovative molecular assay and the associated hardware to rapidly signal the presence of certain targets using reporter sequence found in their genome without requiring PCR. This assay coupled the sensitivity of single-pair fluorescence resonance energy …
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Study of poly(phenylene ethynylene)s, dendritic quenchers, and molecular beacons towards the application of a poly(phenylene ethynylene)-based biosensor
… transduction, and (3) a DNA sequence known as a molecular beacon (MB) selective for Escherichia coli 0157:H7. In order to continue expanding the scope of PPEs in sensor applications, new handles are required with which to manipulate the polymers. These handles should be designed so as to tether …
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Dynamics of DNA Breathing and Folding for Molecular Recognition and Computation
… thesis is centered on the development of the molecular beacon, as a new DNA probe for DNA genotyping, DNA computation and biophysical studies of DNA conformations. Molecular beacons are single-stranded DNA molecules that form a stem-and-loop structure. A fluorophore and a quencher are grafted …
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Use of luminescence energy transfer probes to detect genetic variants.
… this research was to study the hybridization of molecular beacons under different conditions and designs. Data collected suggest that the inconsistency found in the emission intensity of several of these probes may be caused by 3 important factors: length of the probe, nucleotide sequence and, …
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Biomimetic sensor for in-vivo applications
… development of sensitive specific devices (e.g., molecular sensors) for the detection, diagnosis and monitoring of diseases, drug discovery, environmental detection of pollutants and/or biological agents has been an active area of research. A molecular sensor is a biomolecule or biomimetic …
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Metallkomplex-funktionalisierte Oligonucleotide und Peptidnucleinsäuren für die DNA-Sequenzerkennung
… die Reaktionsgeschwindigkeit bis zu 15fach. �Molecular Beacons� (Molekulare Leuchtfeuer) sind Oligonucleotid-Sonden mit Haarnadelstruktur, die bei Hybridisierung fluoreszieren, da eine intramolekulare Fluorophor-Quencher-Wechselwirkung unterbunden wird. Im Rahmen der Arbeit wurden �Molecular …
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Microfluidic chips for combinatorial screening applications
… 4, the combinatorial chip was integrated with a molecular beacon patterned glass cover slip capable of detecting complimentary DNA fragments. Total internal reflection fluorescence (TIRF) microscopy is used for read out of the chip. Four virus-like oligomer targets with sequences corresponding to …
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Synthesis and Spectroscopic Studies of Substituted Pyrrolocytidines
… of modified nucleosides for potential use as molecular probes in nucleic acid chemistry. These heterocyclic base surrogates that are capable of canonical base pairing have use in the study of nucleic acid conformation, as reporters of the state of hybridization.[1] The synthesis of a …
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Sub-Picomolar Detection of SARS-CoV-2 RBD via Computationally-Optimized Peptide Beacons
… a deep learning-based protein design to engineer molecular beacons that function as conformational switches for high sensitivity detection of the SARS-CoV-2 spike protein receptor-binding domain (SRBD). The beacons contain two peptides, together forming a heterodimer, and a binding ligand between …
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Functionalization of Peptide Nucleic Acids via post-synthetic click chemistry
… Nucleic Acid (PNA) has shown great potential in molecular diagnostics, antisense/antigene therapy and nanotechnology. Like other synthetic nucleic acids and artificial analogues, PNA has been extensively modified to achieve better performance in these applications. To efficiently develop PNA …
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Computationally Designed Peptide Binder and Molecular Beacon for SARS-CoV-2
… and experimental mutagenesis. We also present a molecular beacon that detects SARS-CoV-2 spike protein through a conformational switch. Our molecular beacons contain two peptides that can form a parallel heterodimer and a binding ligand between them to detect the SARS-CoV-2 spike protein. A …
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Design and construction of an apparatus for induction heating for controlling DNA hybridization
… to selectively heat nanoparticles attached to "molecular beacons" or DNA loop/hairpin structures. Testing was conducted to see if the heat would be sufficient to open the molecular beacon by dehybridizing the dsDNA. This was accomplished by developing a series of seven coils that were tested …
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Fluorescence imaging microscopy studies on single molecule diffusion and photophysical dynamics
… to employing single fluorophores as "molecular beacons" to determine the position of biomolecules, single molecule fluorescence studies allow to access the photophysical dynamics of genetically encoded fluorescent proteins itself. However, in order to gain statistically consistent …
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Development of luminescent probes for ultrasensitive detection of biopolymers, their complexes, and living cells
… DNA detection using luminescently labeled molecular beacons hybridization probes was performed with sensitivity less then 1 pM, which is about 100 fold higher then that for conventional fluorescent probes. Further 30-fold increase in detection sensitivity was achieved by tethering multiple …
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Fabrication of DNA microarrays on Poly (methylmethacrylate) substrates for biomolecular reporting
… were utilized for immobilizing hairpin probes (molecular beacons) in a microarray format that were used to report on the analysis of complementary DNA (cDNA) specific for fruitless (fru) and ods-site homeobox (OdsH) genes extracted from Drosophila Melanogaster fruit flies. To further improve the …
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Advances in Microfluidics and DNA Nanotechnology for Biomolecular Analysis
… their interactions are probed as biomarkers and molecular machineries key to the analysis of system state and function. However, the tools predominantly employed for the solution-phase study of biomolecules often operate under non-physiological conditions, and are poorly suited to the analysis of …
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Konfokale Mehrfarben Einzelmolekülspektroskopie in Mikrokanälen zur Charakterisierung biologischer Proben
… analysis can be applied to detect and to analyze molecular binding events, as demonstrated in this thesis. The great advantage of this method in comparison to the widely used FCS-method is its sensitivity which lies in the sub-pM regime, as successfully demonstrated in this thesis. I performed …
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Neue fluoreszierende Copolymere für sensitive Detektionssysteme in Wasser
… bereits vielfach in der Biologie in sogenannten „Molecular Beacons“ ausgenutzt, wobei ein Fluorophor und ein Löscher durch spezifische DNA Basenpaarung in einen vorgegebenen Abstand zueinander gezwungen werden und so ein „Schalten“ der Fluoreszenz ermöglichen. Aufgrund der vorgegebenen Struktur …
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Genome analysis: mutation analysis using near infrared laser-induced fluorescence (NIR-LIF) and single molecule detection in microfluidic devices
… NIR system was in a new strategy for analyzing molecular signatures of disease states in real-time using single-pair fluorescence energy transfer (spFRET) coupled with ligase detection reaction (LDR) to rapidly detect single base mutations in codon 12 of K-ras oncogene which has high diagnostic …
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