Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"molecular beacon"”.
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Computationally Designed Peptide Binder and Molecular Beacon for SARS-CoV-2
… and experimental mutagenesis. We also present a molecular beacon that detects SARS-CoV-2 spike protein through a conformational switch. Our molecular beacons contain two peptides that can form a parallel heterodimer and a binding ligand between them to detect the SARS-CoV-2 spike protein. A …
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Optimization of Molecular Beacon-Based Multicomponent Probes for Analysis of Nucleic Acids
Detection of single nucleotide substitutions (SNS) in DNA and RNA has a growing importance in biology and medicine. One traditional approach for recognition of SNS takes advantage of hybridization probes that bind target nucleic acids followed by measuring ?Tm, the difference in melting …
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Dynamics of DNA Breathing and Folding for Molecular Recognition and Computation
… thesis is centered on the development of the molecular beacon, as a new DNA probe for DNA genotyping, DNA computation and biophysical studies of DNA conformations. Molecular beacons are single-stranded DNA molecules that form a stem-and-loop structure. A fluorophore and a quencher are grafted …
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Single molecule studies of rna-target interactions
… two instances, a fluorophore-binding aptamer and molecular beacon, where the folding of RNA can be used to detect an analyte. We have demonstrated that binding of the fluorophore, TAMRA, to an aptamer enhances the fluorescent properties of the fluorophore. Specifically, it increases both its …
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Exploring the Structural and Functional Characteristics of a Catalytic DNA
… applications was studied for Pb2+ detection. The molecular beacon DNAzyme biosensor was shown to be capable of detecting Pb2+ in soft to moderately hard water sources when used in conjunction with a handheld fluorometer. Detection limits under these conditions were lower than 72 nM, which is the …
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Fluorescent and Colorimetric Biosensors Based on DNAzymes and DNA Aptamers
… advanced remarkably. Two primary examples are molecular beacon-based fluorescent detection and nanoparticle-based colorimetric detection. Therefore, combining the two fields opens new avenues to obtain highly sensitive and selective biosensors for a broad range of analytes in applications such …
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IDENTIFICATION AND QUANTIFICATION OF IONIC POLLUTANTS IN ENVIRONMENTAL WATER
… environmental water. In chapter 2, a DNA-based molecular beacon fluorescent sensor was demonstrated for the detection of a cationic pollutant, namely Hg2+, with tunable detection range. This sensor can easily detect Hg2+ in the presence of other metal ions. It is potentially useful for on-site …
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Study of poly(phenylene ethynylene)s, dendritic quenchers, and molecular beacons towards the application of a poly(phenylene ethynylene)-based biosensor
… transduction, and (3) a DNA sequence known as a molecular beacon (MB) selective for Escherichia coli 0157:H7. In order to continue expanding the scope of PPEs in sensor applications, new handles are required with which to manipulate the polymers. These handles should be designed so as to tether …
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DEVELOPMENT OF A RAPID DIAGNOSTIC ASSAY FOR THE COLORIMETRIC DETECTION OF BACTERIAL AND VIRAL PATHOGENS USING LOOP-MEDIATED ISOTHERMAL AMPLIFICATION COUPLED WITH A PEPTIDE NUCLEIC ACID AND GOLD NANOPARTICLE REPORTER SYSTEM
… detection, including culture, immunochemical, or molecular-based techniques, are subject to significant limitations that restrict their clinical utility. Point-of-care (POC) tests have received attention for the diagnostic screening of infectious disease, with benefits of simplicity, …
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Scalable methods for immune repertoire sequencing
… lysed in solution and uses barcoded beads as a molecular beacon to record spatial proximity. Preliminary results suggest the barcoded transcripts produced have the potential to recover TCR pairings at scale.
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Design and construction of an apparatus for induction heating for controlling DNA hybridization
… to selectively heat nanoparticles attached to "molecular beacons" or DNA loop/hairpin structures. Testing was conducted to see if the heat would be sufficient to open the molecular beacon by dehybridizing the dsDNA. This was accomplished by developing a series of seven coils that were tested …
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Highly sensitive biosensors using photonic crystal enhanced fluorescence
… the PCEF platform was also adapted to a molecular beacon assay for miRNA detection, where the PC surface was incorporated with a submicron-height fluid channel. The use of the PCEF platform results in a reduction of the detection limits and an increase in the signal-to-noise ratio (SNR) …
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Microfluidic chips for combinatorial screening applications
… 4, the combinatorial chip was integrated with a molecular beacon patterned glass cover slip capable of detecting complimentary DNA fragments. Total internal reflection fluorescence (TIRF) microscopy is used for read out of the chip. Four virus-like oligomer targets with sequences corresponding to …
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Towards understanding the mechanisms behind mRNA localisation in growing axons
… Xenopus laevis retinal ganglion cell axons using molecular beacon technology coupled with highly inclined and laminated optical sheet (HILO) microscopy. By combining quantitative single molecule imaging with biophysical motion models, I show that β-actin mRNA travels mainly as single copies, and …
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Single-molecule detection of molecular beacons generated from LDR on thermoplastic microfluidic device for bioanalysis
… This research aims to develop an innovative molecular assay and the associated hardware to rapidly signal the presence of certain targets using reporter sequence found in their genome without requiring PCR. This assay coupled the sensitivity of single-pair fluorescence resonance energy …
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Design, Synthesis and Study of Novel Nucleobase Analogs
… properties such that they may be used as biomolecular tools to investigate nucleic acid structure and dynamics. To that end, a derivative of 5-aminouracil (5-AU) labelled with the amine-reactive chromophore 9-chloroacridine was prepared for the purpose of investigating its potential as a …
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Esterase 2-oligodeoxynucleotide conjugates as enzyme reporter for electrochemical detection of DNA and identification of bacterial species
… a further optimization, a stem-loop structured molecular beacon with 5’-thiol and 3’-biotin modifications was synthesized and tested on the chip using EST2-streptavidin as reporter. The presence of target oligodeoxynucleotides complementary to the whole stem-loop sequence enhanced signal for a …
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A Binary Approach for Selective Recognition of Nucleic Acids and Proteins
… whereas the second domain is complementary to a molecular beacon (MB) probe. Binding of both probe strands to the fully matched analyte generates a full MB probe recognition site, allowing a MB to bind and report the presence of the target analyte. Importantly, we show that the OC sensor …
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Functionalization of Peptide Nucleic Acids via post-synthetic click chemistry
… Nucleic Acid (PNA) has shown great potential in molecular diagnostics, antisense/antigene therapy and nanotechnology. Like other synthetic nucleic acids and artificial analogues, PNA has been extensively modified to achieve better performance in these applications. To efficiently develop PNA …