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Showing 1 to 4 of 4 for “"modular cloning"”.
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Genetic mechanisms contributing to antimicrobial resistance: gene repression in Mycobacterium, gene transfer in Salmonella, and phage sensitivity in Escherichia
… multigene constructs on a plasmid with the modular cloning (MoClo) toolkit, which allowed us to control the expression of mmpT5 with the lacZ promoter and monitor mmpSL promoter activity with a fusion to lux. Consistent with the hypothesis, induction of mmpT5 reduced expression of mmpSL. The …
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Creation of Versatile Cloning Platforms for Transgene Expression and Epigenome Editing and Their Application to Pancreatic Islet Biology
… To overcome this, we have created a new modular cloning system (pMVP) that allows a gene of interest to be rapidly recombined in the context of an array of promoters, N- or C-terminal epitope tags, inducible gene expression modalities, and/or fluorescent reporters, into 18 custom …
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Applications of yeast display in synthetic biology with a focus on biological synthesis and recycling of plastics
… synthesis and recycling of plastics. The yeast modular cloning toolkit (MoClo-YTK) utilizes type IIS restriction enzymes for the hierarchical assembly of expression vectors from standardised parts into Saccharomyces cerevisiae expression cassettes. In Chapter 3, to facilitate the use of this …
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Synthetic Auxin Engineering: Building a Biofoundry Platform
… package by providing protocols for mutant and modular indexed plasmid library assembly. Finally, I describe a modular indexed plasmid library (toolkit) for rapid assembly of auxin circuit variants and validate it by building and optimizing an auxin circuit.