Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 18 of 18 for “"mobile genetic elements (MGEs)"”.
-
Helitrons are enriched in lichenized fungi with long generation length and small distribution sizes
<p>Mobile genetic elements (MGEs) have the potential to drive genome evolution by introducing mutations and causing structural instability and chromosomal rearrangements, particularly under conditions like environmental or genetic stress. In this study, we generated 18 new long-read reference …
-
Understanding microbial communities through horizontal gene transfer
… of a previously uncharacterised class of mobile genetic elements (MGEs) into SBW25. Further experiments demonstrated that the isolated MGEs enhance host fitness by providing defence against phage predators, and hijack jumbo bacteriophages for intercellular transfer. Together, this thesis …
-
Synthetic biology tools for sensing and evolving microbes: CRISPR-diagnostics and transposon-mediated genome re-wiring
Mobile genetic elements (MGEs) have played a fundamental role in the evolution of complex organisms, driving innovation through competition, collaboration and co-option. Transposons, in particular, are an ancient family of MGEs whose diverse functions have provided a rich source of DNA-binding and …
-
The evolutionary ecology of methylation-dependent bacterial defense systems
Mobile genetic elements (MGEs) are central to microbial evolution, acting both as vectors of host genomic innovation by transferring adaptive genes between lineages, and as parasites, infection by which has driven the evolution of diverse host defense systems. Although recent years have seen a …
-
Anti-CRISPRs and CRISPR-Cas: Characterization and Biotechnology
… effectively protects against the transfer of mobile genetic elements (MGEs) such as bacteriophages. CRISPR-Cas systems use an RNA-guided nuclease to bind and cleave foreign DNA, presenting a powerful barrier to phage infection. This strong evolutionary barrier led phages to evolve small …
-
Interplay between an integrative and conjugative element and its bacterial host
… mechanism by which bacteria acquire new genes. Mobile genetic elements (MGEs) are the primary drivers of horizontal gene transfer, introducing large fragments of DNA. Often, MGEs contain cargo genes beneficial to the host, conferring new traits such as antibiotic resistances, pathogenicity, and …
-
Phenotypic and genetic diversity of pseudomonads associated with the roots of field-grown canola
… complex and the P. putida complex. Phylogenetic analysis of the partial gene sequences suggested that the phylogeny of root-associated pseudomonads had no effect on their associations with different cultivars or root zones (i.e. rhizosphere and root interior). Principal component analysis …
-
The Comparative Risk of Antimicrobial Resistance Transfer from Pig, Poultry and Bovine Manure to Grassland
… bacteria, antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) into the environment as well as altering the indigenous resistome and microbiome. Grassland consists of ~70% of global agricultural land and is a vital source of food for livestock. The phyllosphere of plants is an …
-
Mobile genetic elements as modulators of the human gut symbiont Bacteroides
Mobile genetic elements (MGEs) facilitate horizontal gene transfer (HGT) in all cellular hosts. In microbial systems, MGEs (e.g. phages, integrative and conjugative elements) are potent drivers of host evolution. Within hosts from the bacterial genus Bacteroides, one of the most common and abundant …
-
A genomic analysis of methicillin resistant Staphylococcus aureus from bovine milk and the discovery of a novel streptococcus species
… of bovine mecC MRSA in the UK. S. aureus Mobile Genetic Elements (MGEs) often encode virulence and antimicrobial resistance genes that can contribute to the pathogenicity of the strain. Acquisition or loss of MGEs and variations within the core genome are reported to play an important role …
-
Intra-species genomic variations in hydrogen-oxidising bacteria is delineated by horizontal gene flow
… by analysing the presence and function of mobile genetic elements (MGEs) in its different populations. The lack of spatial and functional overlap between MGEs and horizontally swept regions confirms that homologous recombination drives horizontal gene flow in Aquificota populations. This …
-
A tripartite survey of CRISPR-Cas diversity: Assessing the contours and limits of natural variation in CRISPR-Cas systems by associated genes and spacer acquisition biases
… homology to anti-phage defence genes and mobile genetic elements even with low CRISPRicity scores, which traditionally been used as the gold standard for proof of co-association with CRISPR-Cas subtypes. A small number of associated antiphage defence genes such as HicAB and DrmB were also …
-
A tripartite survey of CRISPR-Cas diversity: Assessing the contours and limits of natural variation in CRISPR-Cas systems by associated genes and spacer acquisition biases
… homology to anti-phage defence genes and mobile genetic elements even with low CRISPRicity scores, which traditionally been used as the gold standard for proof of co-association with CRISPR-Cas subtypes. A small number of associated antiphage defence genes such as HicAB and DrmB were also …
-
Determination of the occurrence, virulence genes, and antimicrobial resistance profiles of enterococcus faecalis and enterococcus faecium isolated from poultry of Chongwe, Lusaka, Kitwe, and Ndola districts, farmworkers from the Copperbelt province poultry farms and clinical specimens from Kitwe teaching hospital in Zambia.
Enterococcus has great genetic plasticity and is highly versatile, hence does not only acquire and express mobile genetic elements (MGEs) but also disseminates them to pathogenic and non-pathogenic species of the same or of different genera. As such, it has been associated with various clinical …
-
Use of molecular and bioinformatics tools for developing methods of epidemiological identification of trypanosomes
… which is based on variations in position of mobile genetic elements (MGEs). Although commonly used, these tools have not been fully validated. For example, the ITS-PCR has not been used or validated against blood samples obtained from sleeping sickness patients in Angola. Furthermore, the …
-
Conjugative transfer and expression of an integrative and conjugative element in Pectobacterium atrosepticum : A thesis submitted in partial fulfilment of the requirements for the Degree of Doctor of Philosophy at Lincoln University
… credited to the almost ubiquitous association to Mobile Genetic Elements, the means of Horizontal Gene Transfer enabling rapid exchange of genetic information between bacterial populations. Integrative and Conjugative Elements (ICEs) are Mobile Genetic Elements (MGEs) that are transferred largely …
-
A streamlined Workflow for Transposon Mutagenesis in Corynebacterium glutamicum Based on Third-Generation Sequencing and its Application for Advanced Genetic Screens
… Nanopore Technologies (ONT) nanopore Sequencing. Mobile genetic elements (MGEs) destabilize the genetic material of their hosts. While this can contribute to evolutionary plasticity in nature, it is often detrimental in a research and industrial context. Therefore, this work presents and builds …
-
Molecular detection, quantification and characterization of extended-spectrum β-lactamase (ESBL)- and AmpC-encoding genes in a cucumber agroecosystem
… commensal and human pathogenic bacteria using mobile genetic elements (MGEs) such as plasmids. Treatment of the abovementioned infections with currently available antibiotics may thus become ineffective or fail. When this happens, more expensive medicines will have to be utilised and failure to …