Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 16 of 16 for “"mitotic spindles"”.
-
Small Molecules as Probes for Cell Division and Intracellular Transport
… in spindle geometry, chromosome positioning and mitotic index. Monopolar mitotic spindles were induced by 4 inhibitors including one that targets Polo-like kinases, evolutionarily conserved serine/threonine kinases. Using chemical inhibitors and high-resolution live-cell microscopy, I found that …
-
The Role of LIM Kinase 1 and its Substrates in Cell Cycle Progression
… of p27Kip1 expression. LIMK1 is involved in the mitotic process through inactivating phosphorylation of Cofilin. Aurora kinase A (Aur-A), a mitotic kinase, regulates initiation of mitosis through centrosome separation and proper assembly of bipolar spindles. Phosphorylated LIMK1 is recruited to …
-
Synthesis and Molecular Pharmacology of the Diazonamides
… determined that the diazonamides are potent antimitotic agents that induce the formation of mono-aster mitotic spindles. The synthesis of numerous analogs established structure activity relationships which guided the preparation of biotinylated, fluorescent, and radiolabeled forms of the natural …
-
Functional Analysis of Wild-Type LGN and T450 Mutants
… role in cell polarity and alignment of the mitotic spindles and in its absence, the organism ceases to develop. In breast cancer, LGN is upregulated due to phosphorylation of T450 and the knockdown of LGN activity has been shown to suppress growth of breast cancer cells. Furthermore, a …
-
Characterizing the cargo binding and regulatory function of the tail domain in Ncd motor protein
… in the assembly and stability of meiotic and mitotic spindles in Drosophila oocytes and early embryos, respectively. Ncd functions by cross-linking microtubules through the tail and motor domains. It was originally believed that the role of the Ncd tail domain was to only statically bind …
-
The role of cell division during tissue morphogenesis
… to three-dimensional embryo expansion. Here, the mitotic spindles are oriented parallel to each other and perpendicular to the previous cell division plane. A stark asymmetry in spindle pole size creates a directionality in this spindle positioning, where the larger spindle pole points towards the …
-
Ncd Motor Tail Domain Interactions With Microtubules
… and "cargo" microtubules (MTs) in meiotic and mitotic spindles through the action of ATP-dependent and ATP-independent MT binding sites in the head and tail domains, respectively. Through the action of these binding sites, Ncd bundles and, perhaps, slides MTs relative to each other. These …
-
Regulation of Progression through Unperturbed Mitosis and in the Presence of Environmental Stress
… of the genome. Surveillance pathways like the mitotic spindle checkpoint have evolved to prevent mitotic defects effectively. The molecular basis of these regulatory mechanisms is particularly well understood in budding yeast. It is not entirely clear to what extent these findings are valid for …
-
Sulforaphane Inhibits Mammary Cancer Cell Mitotic Progression and Tubulin Polymerization
… in elevated numbers of prophase/prometaphase mitotic figures, indicating cell cycle progression beyond G2 and arrest early within mitosis. Moreover, cells treated with 15 muM SUL displayed aberrant mitotic spindles, and higher doses of SUL inhibited tubulin polymerization in vitro. In …
-
Bioinformatics Analysis and Annotation of Microtubule Binding and Associated Proteins (MAPs) - Creating a Database of MAPs
… bodies, centrioles, flagellum, kinetochores, and mitotic spindles). The changing roles require the cytoskeleton to be both dynamic and static in nature. Guiding these processes are a network of proteins that direct cellular behavior through their ability to bind microtubules (MTs) in a spatial- …
-
Microtubule Organisation in Normal and Colon Cancer Cells
… Additionally,"the"angle"of"the"mitotic"spindles"was"analysed"in"the"different"cell"lines." The" impact" of" EB2" loss" on" microtubule" organisation" was" determined" by" immunolabelling" following" siRNA" treatment." "A" fluorescently" tagged" EB2" construct" …
-
Using Microscopy to Examine Mechanisms of Cell Division
… requires the assembly of a microtubule-based mitotic spindle. In the research presented here, I examined two aspects of mitotic spindle morphogenesis. First, I looked at how chromosomes attach to microtubules in mitosis. The capture of microtubule plus-ends by kinetochores (the site of …
-
Breaking Down Microtubule Formation: Characterizing the Biochemical and Cellular Functions of the γ-Tubulin Ring Complex
… well as γ-tubulin complex proteins 2-6 (GCP2-6), mitotic-spindle organizing proteins associated with a ring of γ-tubulin proteins 1 and 2 (MOZART, or MZT1 and MZT2), and an actin molecule. This ~2.2MDa assembly regulates microtubule dynamics by facilitating the nucleation of new microtubules, …
-
Identification and characterisation of FAM83 proteins as key regulators of CK1 protein kinases in cells: FAM83D recruits CK1α to the mitotic spindle for proper spindle positioning
… the only FAM83 member known to localise to the mitotic spindle. RNA interference approaches targeting FAM83D have been reported to impact chromosome alignment and cause a delay in the timings of cell division. Whilst all FAM83 proteins appear to associate robustly with the CK1 alpha isoforms …
-
Investigating Differentiation: A Role for Organelle Inheritance in Epidermal Growth
… essential for life. Further study revealed mitotic changes associated with Pex11b-deficient basal progenitors including a mitotic delay, during which spindles rotate uncontrollably, perturbing polarized divisions and skewing daughter fates. Probing deeper, we discovered that without PEX11b, …
-
A study of novel tools to measure and perturb Aurora Kinase A protein stability
… mitosis by promoting centrosome maturation and mitotic spindle assembly. The cognate pathway of AurKA degradation is through the ubiquitin proteasome system (UPS), a series of catalytic steps including the APC/C E3 ligase, resulting in the ubiquitination and subsequent degradation by the …