Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"mitotic spindle assembly"”.
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Regulation of Mitotic Spindle Assembly in Caenorhabditis elegans Embryos
The mitotic spindle is a bipolar microtubule-based structure that mediates proper cell division by segregating the genetic material and by positioning the cytokinesis cleavage plane. Spindle assembly is a complex process, involving the modulation of microtubule dynamics, microtubule focusing at …
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Molecular Mechanisms of Mitotic Spindle Assembly and Accurate Chromosome Segregation
… proteinaceous structures built on centromeres of mitotic chromosomes that act as the attachment site for microtubules, serve as major forces to position each pair of chromosomes to the metaphase plate. Secondly, a surveillance system, known as the mitotic checkpoint, put the anaphase onset on hold …
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B-cyclin/CDK Regulation of Mitotic Spindle Assembly through Phosphorylation of Kinesin-5 Motors in the Budding Yeast, <italic>Saccharomyces cerevisiae</italic>
… years that B-cyclin/CDK complexes regulate the assembly of the mitotic spindle and entry into mitosis, the full complement of relevant CDK targets has not been identified. It has previously been shown in a variety of model systems that B-type cyclin/CDK complexes, kinesin-5 motors, and the …
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SMARCA4/BRG1-Inactivating Mutations as Potential Predictive Markers for Aurora Kinase A-Targeted Therapy in NSCLCs
… Thus, AURKA-centered, centrosome-independent, mitotic spindle assembly machinery becomes solely responsible for mitotic spindle formation and proper chromosome segregation during mitosis. DLG7, the only known protein specific to this centrosome-independent mitotic spindle assembly, is required …
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Identification and Analysis of A Novel Role For The to usled-Like Kinase In Regulating Mitotic Spindle Dynamics
… by poor metaphase chromosome alignment, delayed mitotic progression, lagging chromosomes, and supernumerary centrosomes.</p> <p>I discovered an unanticipated requirement for TLK-1 in mitotic spindle assembly and positioning. Normally, in the newly-fertilized zygote (P<sub>0</sub>) the maternal …
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Structural and Molecular Mechanisms of Centrosome Assembly and Strength
… material (PCM), organizes microtubules for mitotic spindle assembly. The molecular interactions that enable PCM to assemble and resist external forces are poorly understood. To answer such question, we use cross-linking mass spectrometry (XL-MS) to analyze PLK-1-potentiated multimerization …
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Synthesis and Molecular Pharmacology of the Diazonamides
… determined that the diazonamides are potent antimitotic agents that induce the formation of mono-aster mitotic spindles. The synthesis of numerous analogs established structure activity relationships which guided the preparation of biotinylated, fluorescent, and radiolabeled forms of the natural …
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Functional characterisation of cohesin subunit SMC3 and separase and their roles in the segregation of large and minichromosomes in Trypanosoma brucei
… and segregated by association with the mitotic spindle, minichromosomes exhibit segregation patterns that differ from those observed for large chromosomes. To address whether this difference is reflected at a molecular level, two different proteins that have highly conserved functions in …
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Identification of centrosomal proteomes using COMPACT, a novel tool for purification of centrosomes
… role in multiple cellular processes; from mitotic spindle assembly and cell division, to cell migration, invasion and polarity. The human centrosome consists of two cylindrical structures known as centrioles, decorated at their distal ends by appendage structures, and surrounded by a …
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Investigating the effect of a small molecule, Inhibitor of Nuclear Import (INI-43) as a pan-cancer treatment
… in other cellular processes such as mitosis and mitotic spindle assembly, DNA replication and cell cycle progression. Previous studies in our laboratory have reported that KPNβ1 is overexpressed in cervical and oesophageal cancer and is important for cancer cell survival and function, suggesting …
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Determining how variations in cell and nuclear size contribute to mitosis and tumorigenicity in cancer cells that undergo whole genome doubling
… in cell and nuclear size after WGD affect mitotic fidelity and tumorigenic potential. I show that cell and nuclear size do not always scale with DNA content in cancer cells that undergo WGD, resulting in 4N cancer cells that vary in size. With this experimental system, I demonstrate that …
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A study of novel tools to measure and perturb Aurora Kinase A protein stability
… mitosis by promoting centrosome maturation and mitotic spindle assembly. The cognate pathway of AurKA degradation is through the ubiquitin proteasome system (UPS), a series of catalytic steps including the APC/C E3 ligase, resulting in the ubiquitination and subsequent degradation by the …