Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"mitotic progression"”.
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CDK control of mitotic progression in Saccharomyces cerevisiae
Mitotic cyclin-dependent kinases (CDKs) are best known for their essential functions in triggering entry into M-phase, where they have established roles in nuclear envelope breakdown, chromosome condensation, and Golgi fragmentation. Whether mitotic CDKs have essential functions within mitosis is …
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Sulforaphane Inhibits Mammary Cancer Cell Mitotic Progression and Tubulin Polymerization
… in elevated numbers of prophase/prometaphase mitotic figures, indicating cell cycle progression beyond G2 and arrest early within mitosis. Moreover, cells treated with 15 muM SUL displayed aberrant mitotic spindles, and higher doses of SUL inhibited tubulin polymerization in vitro. In …
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TO INVESTIGATE THE ROLE OF RNF8 IN REGULATING MITOTIC PROGRESSION IN GLIOMAS
… RNF8, but not the FHA or RING mutant, blocks GSC mitotic progression and augments genome instability. Indeed, low RNF8 expression portends poor patient prognosis in glioma patients. Importantly, our mechanistic findings led to the rational combination of PLK1 and HSP90 inhibitors that elicit an …
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Modulation of mitotic progression and cell cycle checkpoints by phosphorylation-dependent protein-protein interactions
Alteration of mitotic gene function has recently been discovered to play a key role in tumor formation and cancer progression through the induction of chromosomal aberrations and genomic instability. Polo-like-kinase-1 is a critical mitotic regulator, overexpressed in human tumors, that functions …
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Analyzing Sumo-2/3 Modification In Regulation Of Breast Cancer Progression And Mitotic Chromosome Segregation
<p>Roles of SUMOylation in cancer metastasis and mitotic progression has been elucidated in this thesis. This vital and dynamic post-translational modification has been known to be dysregulated in several kinds of cancer. Global SUMO-2/3 conjugation is upregulated in a mouse metastatic breast …
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Mitotic slippage by ATP depletion and its mechanism
… the existence of energy checkpoint during mitotic progression. In our hand, ATP depletion by the co-treatment with 2-deoxy glucose (2-DG) and sodium azide (NaN3) during interphase caused robust cell cycle arrest, supporting the existence of energy checkpoints sensing shortage of ATP supply …
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DECIPHERING THE FUNCTION OF HURP IN REGULATING MICROTUBULE DYNAMICS AND CHROMOSOME MOVEMENT
The regulation of mitotic spindle dynamics is important for faithful chromosome segregation. HURP is an important microtubule-associated protein for stabilizing and bundling the mitotic spindle. Depletion of HURP delays the mitotic progression, but the underlying mechanism remains unknown. Our …
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Exploring the Regulation of Mitotic PP2A-Rts1 Activity in Saccharomyces cerevisiae
… processes in all organisms. Particularly, mitotic onset is regulated in all eukaryotes by an increase in cyclin-dependent kinase 1 (Cdk1) activity caused by the dephosphorylation of Cdk1 on a conserved tyrosine residue. PP2ARts1 is a phosphatase that participates in dephosphorylating the …
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Identification and functional characterisation of novel APC/C interacting proteins
… APC/C and TIF1\(\gamma\) cooperate to regulate mitotic progression. Notably TIF1\(\gamma\) displays in vivo interactions with APC/C subunits 1-8, the APC/C‟s mitotic coactivator CDC20 and mitotic substrate cyclin A. Moreover, TIF1\(\gamma\) depletion by RNAi arrests cells in a metaphase-like …
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The Apical Polarity Complex Protein, Pals1, Regulates Cell Fate In The Development of Cerebellum and Neocortex
… mitosis as Pals1 deletion causes the delay of mitotic progression and incomplete chromosome segregation. Additionally, we uncovered a crucial downstream factor mediating Pals1 function, Pttg1, which is known as an essential protein for sister chromatid segregation during mitosis. Thus, our …
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Unbiased global proteomic profiling of patient-derived meningiomas of all grades to identify molecular signatures of differentially expressed proteins and phosphoproteins.
… as grade-specific candidates relevant for tumour progression. Top candidates were validated by Western blotting and immunohistochemistry in an independent sample set, confirming for example significant overexpression of proteins including EGFR, STAT2 and EPS8L2 across all grades, as well as the …
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Molecular mechanisms of kinetochore microtubule attachment
… The Astrin-SKAP-LC8 complex is essential for mitotic progression and directly associates with microtubules. Furthermore, the microtubule polymerization factor CLASP requires the Astrin-SKAP-LC8 complex to localize to kinetochores. Second, we demonstrate that the Skal complex has many of the …
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The role of cell division during tissue morphogenesis
… to three-dimensional embryo expansion. Here, the mitotic spindles are oriented parallel to each other and perpendicular to the previous cell division plane. A stark asymmetry in spindle pole size creates a directionality in this spindle positioning, where the larger spindle pole points towards the …
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Cyclin B1 Mediates The Effect of Uchl1 In Promoting Cell Cycle Progression In Uterine Papillary Serous Carcinoma
… that UCHL1 contributes to UPSC tumor progression by modulating the protein stability of target genes.</p> <p>To test this hypothesis, we first examined the functional role of UCHL1 in UPSC progression. Subsequently, we found that UCHL1 silencing reduced cell proliferation in vitro and …
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Development of Non-Atp Competitive, Plk1-Selective Inhibitors
… plays an important role in the regulation of mitotic progression. PLK1 is over-expressed in a number of tumor types, thus making it a good therapeutic target. Because other PLK family members are putative tumor suppressors, it is imperative that a PLK1 inhibitor be exclusive for PLK1. By …
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Functional analyses of mitotic microtubule-binding complexes
… microtubules into a bipolar array, termed the mitotic spindle. The depolymerization of kinetochore-bound microtubules generates the force required to properly segregate the chromosomes. The work in this thesis analyzes the molecular basis for the function and activity of two key players in …
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Determining how variations in cell and nuclear size contribute to mitosis and tumorigenicity in cancer cells that undergo whole genome doubling
… size abnormalities are associated with cancer progression and can affect cell physiology by altering gene expression, metabolism, cytoskeletal structure, and proliferation. Prior work in non-transformed and cancer cell lines showed that tetraploid (4N) cells experience a two-fold increase in …
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Analysis of Aurora B Regulation and Signaling
… (APC/C), an ubiquitin ligase essential for mitotic progression. Here, we showed that Aurora B is a substrate of APC/C both in vitro and in vivo. Aurora B is efficiently ubiquitinated iv in an in vitro reconstituted system by APC/C that had been activated by Cdh1. The recognition of Aurora B …
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Polo-Like Kinase 1 and Smooth Muscle Cells in Coronary Artery Bypass Conduits and Coronary Arteries Following Angioplasty
… H3 pathway in mediating mitotic progression of smooth muscle cells (SMCs) from human IMA and SV in the development of IH leading to veingraft disease. We also show in vivo data in support of increased immunopositivity towards PLK1 and pPLK1 in SMCs, following percutaneous …
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The Non-canonical Function and Regulation of TBK1 in the Cell Cycle
… activity often leads to errors in the cell cycle progression which consequently becomes the underlying cause for developmental defects or abnormal cell proliferation leading to cancer. Tank Binding Kinase 1 (TBK1) is overexpressed in certain cancer types and is activated on the centrosomes during …
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