Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"mitotic chromosomes"”.
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Large-Scale Structure of Mitotic Chromosomes
… as forming the bases of DNA loops in metaphase chromosomes. To test the structural role of SAR/MAR sequences, we created stable cell lines containing chromosomes with regions of high density of integrated SAR/MAR sequences. CHO cells were isolated containing multiple copies of a vector …
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Electron microscopic studies of mitotic chromosomes in yeast
… in yeast cell biology is whether or not the chromosomes of the yeast, Saccharomyces cerevisiae, condense during mitosis and, if they do, to what extent they are condensed.
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Quantitative Analysis of the Structural Dynamics of Mitotic Chromosomes in Live Mammalian Cells
Chromatin, organized into individual chromosomes, is the physiological carrier of the genetic and epigenetic information in eukaryotes. In the nucleus of an intact cell, the structure of chromatin is dynamic and essential for genomic activities. The most dramatic changes in chromatin structure …
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A cytogenetic map for the genomic studies of the West Nile Virus vector Culex tarsalis
… genome mapping in Cx. tarsalis and to compare mitotic chromosomes between three species of Culicinae mosquitoes. Using mitotic chromosomes from imaginal discs of 4th instar larvae of Cx. tarsalis, we developed idiograms based on morphology and proportions of the mitotic chromosomes. In …
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Identification and Characterization of Hells-CDCA7, a Nucleosome Remodeling Complex Required for Mitotic Chromosome Structure
<p>The major components of mitotic chromosomes including histones, topoisomerase II and condensin are known to compact and shape the chromatin into rod-like chromatids. However, a complete picture of the proteins involved in shaping mitotic chromatin is unsettled. Here, I perform mass spectrometry …
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Optimization of Cytogenetic and Physical mapping of Culicinae genomes
… Culex genomes, the structure of their polytene chromosomes is damaged by ectopic contacts that make the analysis difficult. Mitotic chromosomes from imaginal discs of 4th instar larvae of Aedes aegypti were tested as a source for the physical genome mapping for this mosquito. Chromosomes in …
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Linker Histone Medicated Regulation of Mitotic Chromosome Compaction and Individualization
<p>Mitotic chromosomes are scaled to the cell size to ensure effective chromosome segregation. Recent studies have shown how condensins and DNA topoisomerase II organize the mitotic chromosome. However, the regulation of these factors in maintaining proper chromosome size in different cell types …
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Assessment of chromatin activity in mouse and human tissues
… of euchromatic and heterochromatic regions of mitotic chromosomes. This ill ~ technique has been used in this study to ask whether facultative heterochromatin of the inactive X chromosome can be distinguished from the active X chromosome in mouse and human tissues. In addition to this, in ~ …
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Characterizing the contribution of the LXCXE binding cleft to pRB-mediated genome stability and tumor suppression
Condensation and segregation of mitotic chromosomes are critical processes for cellular propagation and if compromised, can lead to genomic instability. Genomic instability is known to be an active contributor to tumorigenesis, rather than being a by-product of malignant progression. The …
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Characterization of a Novel Chromatin-Induced Mechanism that Couples Microtubule Disassembly and Nuclear Re-Formation
… polymerization and spindle formation from mitotic chromosomes. Removal of the CPC from chromosomes at anaphase is required for proper nuclear reassembly, but the molecular basis for this requirement remains unclear. On the whole, the orchestration of structural events at the end of mitosis …
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An in vivo study of chromatin compaction in human cells
… interphase chromatin compacts to form the mitotic chromosomes. The arrangement of chromatin within cells, with respect to its compaction state, has a number of implications for processes like transcription, DNA replication, DNA repair and more recently, in vision of nocturnal animals. …
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Molecular Mechanisms of Mitotic Spindle Assembly and Accurate Chromosome Segregation
… proteinaceous structures built on centromeres of mitotic chromosomes that act as the attachment site for microtubules, serve as major forces to position each pair of chromosomes to the metaphase plate. Secondly, a surveillance system, known as the mitotic checkpoint, put the anaphase onset on hold …
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Probing Mitotic Chromosome Structure and Arrangement Using Micromanipulation
… number (seven in the male) for a mammal. Mitotic chromosomes can be removed from cells either by extraction with microneedles or by inducing chromosome expulsion with a drop of silicone oil applied to the cell. The entire diploid complement is removed by virtue of interconnecting fibers …
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Polymer models of chromosomes
Studies of chromosomes have a long history. Since late XIX century, microscopy studies have revealed that chromosomal organization as seen by light microscopy is different among organisms, cell types, or stages of the cell cycle. However, the internal organization of chromosomes at scales below the …
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Evidence that the Boundary Element-Associated Factors BEAF-32A and BEAF-32B affect chromatin structure in Drosophila melanogaster
… BEAF-32B bind to hundreds of loci on Drosophila chromosomes. These proteins function as insulators; they can prevent promoter activation by an enhancer when placed between them and protect transgenes from chromosomal position effects. To gain insight into BEAF function we designed and expressed a …
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Chromosomal evolution in mosquitoes - vectors of diseases
… control. We developed the first map of the mitotic chromosomes of the major vector for West Nile fever and lymphatic filariasis, Culex quinquefasciatus. The map was then successfully utilized for mapping of approximately 90% of available genetic markers to their precise positions on the …
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Studies on the Biology of <i>Striga aspera</i> (Scrophulariaceae) in Nigeria
… host. A new procedure developed for counting mitotic chromosomes in Striga established new chromosome counts of n = 18 for Striga aspera and n = 19 for Striga hermonthica. Hybridization success between both species ranged from 68 to 95%. Seed derived from all crosses were viable, germinable …
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Intrinsic Control Strategies for Herpesvirus-based Vaccine Vectors
… through enabling episome tethering to mitotic chromosomes. The first aim of the project was to investigate the ability of a latency-defective BoHV-4 Δ ORF73 vector expressing heterologous antigens to induce immune responses. Four recombinant defective BoHV-4 Δ ORF73 vectors, each …
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Genome organization by loop extrusion
… propagate in the same way - they duplicate their chromosomes and divide into two daughter cells, each containing one copy of each chromosome. Because of the extreme length of chromosomes, their two copies have be compacted in order to be successfully segregated from each other. The process of …
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Chromosome and Genome Evolution in Culicinae Mosquitoes
… or gene exons from twenty-four scaffolds to the mitotic chromosomes from imaginal discs. This study resulted in the construction of a first physical map of the Ae. albopictus genome as well as mapping viral integration and polyphenol oxidase genes. Moreover, comparing our present Ae. albopictus …
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