Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"mitotic checkpoint"”.
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Molecular Mechanisms of Mitotic Checkpoint Complex Assembly onto Kinetochores
… attachments, the spindle assembly checkpoint (SAC), activates the mitotic checkpoint complex (MCC), to inhibit the E3 ubiquitin ligase, the anaphase-promoting complex/cyclosome (APC/C). Inhibition of the APC/C then delays premature chromosome segregation by preventing APC/C-mediated …
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Role of Chk1 and Chk2 in mitotic checkpoint control in vertebrate cells
… genotoxic stress and mediate multiple cell cycle checkpoint mechanisms that ensure genomic integrity. The establishment of mitotic checkpoint delay in response to DNA damage or incompletely replicated DNA is conventionally thought to be accomplished through inhibition of the cyclin-dependent …
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STING safeguards epithelial genome integrity and protects from carcinogenesis via mitotic checkpoint control
… interferon-independent genome-integrity checkpoint that couples DNA damage signaling to cell-cycle control, thereby restraining chromosomal instability, tumor evolution, and providing a therapeutically exploitable vulnerability in cancer therapy.
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Investigating a novel function of histone H3 in mitotic checkpoint control in Saccharomyces cerevisiae
Thesis (Ph. D.)--Michigan State University. Dept. of Biochemistry and Molecular Biology, 2010
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Detection of Aneuploidy for Chromosomes 7 and 8 Using Fluorescence <i>In Situ</i> Hybridization in Patients with Aplastic Anemia and Sequencing of the Mitotic Checkpoint Gene hBUB1
… these abnormalities.</p> <p><em>hBUB1</em>, a mitotic spindle checkpoint gene, was shown to be mutated in two colorectal cancer cell lines with high levels of aneuploidy (Cahill, et al., 1998). Although theoretically possible, conclusive evidence does not currently exist establishing a link …
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Molecular Mechanisms of Mitotic Spindle Assembly and Accurate Chromosome Segregation
… proteinaceous structures built on centromeres of mitotic chromosomes that act as the attachment site for microtubules, serve as major forces to position each pair of chromosomes to the metaphase plate. Secondly, a surveillance system, known as the mitotic checkpoint, put the anaphase onset on hold …
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Regulation of BubR1 by Ultraviolet Radiation: Implications in Skin Cancer
… of skin cancer. BubR1 is an essential mitotic checkpoint protein that plays a critical role in regulating chromosome segregation and maintaining genomic stability. BubR1 dysregulation can lead to an increase in chromosomal instability and aneuploidy, a common phenomenon associated with …
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BUBR1 in Cardiac Biology: From Cardiogenesis to Adult Heart Function and Aging
… dissertation investigates the role of BubR1, a mitotic checkpoint protein, in cardiac morphogenesis, cardiac aging, intercellular communication, and wound healing, uncovering novel regulatory pathways that contribute to both developmental and degenerative cardiac conditions. Our findings …
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Mechanism of APC/C activation and substrate specificity in mitosis
… first part of this thesis was to understand how mitotic phosphorylation regulates APC/C activity. Using cryo-electron microscopy and biochemical analysis, we found that an auto-inhibitory segment of the Apc1 subunit acts as a molecular switch that in apo unphosphorylated APC/C interacts with a …
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Molecular Mechanisms Involved in Chromosome Segregation
… and couples it to the microtubules (MTs) of the mitotic spindle. In human cells, the details of CCAN-CENP-ANuc assembly are unknown. This thesis investigates the structure and assembly of the human CCAN-CENP- ANuc complex, showing that CCAN tightly grips the linker DNA of the CENPANuc. CENP-C …
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Safeguard of Mitosis: The Spindle Checkpoint
… attachment is under surveillance by the spindle checkpoint to ensure the fidelity of chromosome segregation. Defects in the checkpoint could lead to aneuploidy, which has been implicated in cancers, birth defects, and other human diseases. In presence of kinetochores that are not attached or …
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Characterisation of the KA1 & KA2 domains and interaction with APC/C
… subunit of the APC/C inhibitor complex the mitotic checkpoint complex (MCC). These finding suggest a new role for KA contain kinases as regulators of APC/C activity. Future studies to identify the residues of Mad2 which are phosphorylated by these kinases, as well as the binding site of Slp1 …
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TRAF Regulation of Caspase-2-Dependent Apoptosis in Response to DNA Damage
… cells are equipped with multiple defenses. Checkpoints can sense problems and halt a cell’s progression through the cell cycle in order to allow repairs. More drastically, cells can also prevent passing on mutations to progeny by triggering apoptosis, or programmed cell death. This work will …
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Mitotic slippage by ATP depletion and its mechanism
… known that tumor cells possess two main energy checkpoints monitoring the amount of available ATP during G1 phase and G2 to M transition. However, it is still uncertain the existence of energy checkpoint during mitotic progression. In our hand, ATP depletion by the co-treatment with 2-deoxy …
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Regulated Recruitment of the Chromosomal Passenger Complex to Chromatin and Microtubules Promotes Accurate Cell Division
… attachments and activating the spindle assembly checkpoint (SAC). Both functions require Aurora B activation, which is promoted by its binding to the C terminus of the CPC subunit INCENP, and by localization to the inner centromere, which requires the N terminus of INCENP. Inner centromere …