Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"mitochondrial translation"”.
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The role of RNA modifications in mitochondrial translation
Mitochondrial gene expression entails multiple steps, one of which is translation, performed by the mitochondrial ribosome. Post-transcriptional RNA nucleotide modifications have been identified in mitochondrial (mt)-rRNAs. However, little is known about their role in terms of mitochondrial …
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Defect of Mitochondrial Translation Machinery Enhances Hepatoma Cell Invasiveness
… rate 5 4. Estimation of intracellular and mitochondrial ROS level 5 5. Total genomic DNA isolation and sequencing of mitochondrial DNA fragments 5 6. Western blot analysis 6 7. Real-time reverse transcription-polymerase chain reaction (qPCR) 7 8. Gene expression profiling and data analysis …
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Inhibition of Mitochondrial Translation as a Therapeutic Strategy for Acute Myeloid Leukemia
Inhibition of mitochondrial translation as a therapeutic strategy for acute myeloid leukemia Marko Škrtić Doctor of Philosophy Institute of Medical Science University of Toronto 2012 Abstract Intro: Acute myeloid leukemia (AML) therapies have remained unchanged for 20 years, and thus new therapies …
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Mitochondrial regulation of CD8+ T cell cytotoxicity
… USP30 is a deubiquitinase localised on the outer mitochondrial membrane and on the peroxisomal membrane, where it counteracts mitophagy and pexophagy by cleaving ubiquitin chains from target proteins. Here I characterise T cell motility, signalling, energy requirements and cytotoxicity in CD8+ T …
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AQP1—A REGULATORY FACTOR ASSOCIATED WITH BROWN ADIPOSE TISSUE SILENCING TO COMBAT OBESITY AND METABOLIC DISEASE
… to the electron transport chain (ETC) and mitochondrial translation. Thus, I demonstrate that ablation of AQP1 in BAT protects mice against DIO by increasing whole body energy expenditure through upregulation of the core ETC and mitochondrial translation gene program. My findings identify …
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Biogenesis and Function of the Mitochondrial Ribosome
… into the biogenesis and function of the human mitochondrial ribosome. The mammalian mitochondrial ribosome (mitoribosome) is one of the largest ribonucleoprotein complexes in the cell, with the overall molecular mass of the fully assembled 55S monosome being ~2.7 MDa. It is indispensable for …
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Protein factors involved in the biogenesis of the mitochondrial ribosome
… phosphorylation (OxPhos) system, and the mitochondrial (mt-) rRNAs and tRNAs required for their translation. The maturation of the mitochondrial ribosome requires both mt-rRNAs to undergo post-transcriptional chemical modifications, folding of the rRNA and assembly of the protein …
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RNA-binding proteins in yeast mitochondria
… motifs. Besides analysing their effects on mitochondrial translation and their organisation in protein complexes the work focused on the characterisation of the RNA-binding properties of recombinant Cit1p and Mdh1p: · Cit1p and Mdh1p play no essential role in mitochondrial protein synthesis. …
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Adrenergic control of proteolysis in brown adipocytes
… result in tissue atrophy by loss of cells, mitochondrial proteins, and uncoupling protein 1 (UCP1), the molecular basis for thermogenesis. The overall objective is to gain a better understanding of the control and the mechanisms underlying BAT atrophy. Specific objectives are: (1) whether in …
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Investigating Cell-Type-Specific Vulnerability in Mitochondrial Disease Using Stem Cell-Derived Neuronal Models
Mitochondrial diseases are a group of widely heterogeneous genetic disorders that primarily affect tissues with high energy demands. Although disruption of oxidative phosphorylation is a shared hallmark across these conditions, their clinical manifestations display remarkable tissue specificity, …
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The Role of Mitochondria in the Development of Insulin Resistance and Type 2 Diabetes
… into the potential role of nuclear-encoded mitochondrial (NEM) genes in the aetiology of T2D. A pathway analysis approach is used to test for enrichment of T2D association signals across the genome in defined NEM gene sets. From this analysis, the biological pathways of glycolysis, the …
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IDENTIFICATION OF FACTORS INVOLVED IN DOUBLE STRANDED RNA-INDUCED CELL DEATH THROUGH GENOME-WIDE CRISPR SCREENS
… RNA Polymerase II Transcription Initiation, Mitochondrial translation and respiratory chain and DNA Repair genes have a protective role against dsRNA increase. Among multiple candidates with synergistic/inhibitory role with dsRNA, we validated the most translationally relevant BRCA1, commonly …
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Mitochondrial Quality Control Adaptations Support Malignant Progression of Serous Ovarian Cancer Cells and Spheroids
… invasion through modulation of quality control, mitochondrial dynamics, mitophagy, and mitobiogenesis. We have shown that aggregation of cancer cells supports increased mitochondrial fragmentation localized to the hypoxic core of our spheroid models. Further, aggregation supports enhanced …
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From Protein Structure to Clinical Strategy: An In silico Investigation of Mitochondrial DNA Mutations in Type 2 Diabetes and Degenerative Disc Disease for Targeted Nutritional Interventions
… stress intersect. Increasing evidence identifies mitochondrial dysfunction as a shared mechanistic pathway underlying both metabolic and degenerative disease processes. Mitochondrial DNA (mtDNA), which encodes essential structural subunits of the electron transport chain (ETC) as well as critical …
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Genetic Engineering as a Tool to Investigate Mitochondrial Gene Expression
… polypeptides for the OXPHOS system, alongside mitochondrial rRNAs and tRNAs required for their expression. Gene expression in mitochondria is distinct from the bacterial and eukaryotic cytosolic counterparts, and many aspects of the processes of transcription and translation are not fully …
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Mechanisms and Evolution of Mitoribosomal Small Subunit Biogenesis
… generate functional subunits which exhibit high translational accuracy and processivity. During ribosome biogenesis, ribosomal proteins and a multitude of additional assembly factors, of which there are over 200 in yeast, orchestrate rRNA processing, folding, and modification. While the vast …