Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"mitochondrial targeting"”.
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Mitochondrial targeting of wild-type and mutant human protoporphyrinogen oxidase (PPOX)
Bibliography: 140-197 leaves .
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Helicobacter pylori VacA as a mitochondrial targeted pathogenic effector
… play a central role in life and death. Although mitochondrial dysfunction is at the core of many diseases, the mechanisms and consequences of mitochondrial targeting and dysfunction induced by pathogenic microbes is poorly understood. The vacuolating cytotoxin (VacA) of the human gastric pathogen …
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ELUCIDATION OF MECHANISMS GENERATING 5-HYDROXYMETHYLCYTOSINE (5hmC) IN MAMMALIAN MITOCHONDRIA
… modification in immunoprecipitates of mammalian mitochondrial DNA(mtDNA). We provide the first evidence that a DNA Methyltransferase-1 isoform (mtDNMT1) translocates to the mitochondria using an N-terminal mitochondrial targeting sequence. mtDNMT1 expression is upregulated by NRF1 and PGC1α, …
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The Role of Peroxiredixin Ⅲ in the ototoxic drug-induced mitochondrial apoptosis of cochlear hair cell
… cells. Among them, Prx III is synthesized with a mitochondrial targeting sequence and is then transferred to mitochondria. We have shown that Prx III is an important, if not the most important, H2O2-eliminating enzyme in mitochondria and that the mitochondrial generation of H2O2 contributes to …
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Insights into the Functional Link between MtHsp40:MtHsp70 Network and Mitochondrial Homeostasis
Mitochondrial apoptosis, morphology and function can be modulated by the activity of mitochondrial chaperones including mtHsp40 and mtHsp70. We found that both overexpression and depletion of mtHsp40 reversibly changed mitochondrial morphology from tubular network to fragmented puncta, in a process …
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Lipoic acid metabolism in Leishmania major
… it was predicted that all three proteins possess mitochondrial targeting peptides. Targeting of these proteins to the mitochondrion was verified by a green fluorescence protein (GFP) reporter system, and by subcellular pre-fractionation using digitonin followed by western blotting. Functionality …
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The Role of Parkin-Mediated Mitophagy During Aging and Exercise in Skeletal Muscle
… noted a reduced transcriptional drive towards mitochondrial biogenesis in KO and aged animals. The results demonstrate that acute exercise can selectively increase mitochondrial targeting for degradation, and that this process is dependent on Parkin, as well as with age. We next focused on the …
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Characterization of the Saccharomyces cerevisiae gene MGP1, a novel member of the ras gene superfamily that functions in the mitochondrial biogenesis
<p>This dissertation characterizes Mgp1p, a mitochondrial protein that functions in mitochondrial biogenesis. Mgp1p is the first protein related to the p21[superscript] H-ras oncoprotein shown to function in mitochondria;The Saccharomyces cerevisiae gene MGP1 was isolated based on its ability to …
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Molecular characterization and transcriptional regulation of GCV3, the Saccharomyces cerevisiae gene encoding the H-protein of the glycine cleavage system
… a 177 amino acid residue protein with a putative mitochondrial targeting sequence at its amino terminus. Targeted gene replacement shows that GCV3 is not essential for growth on minimal media. It is, however, essential for growth when glycine serves as the sole nitrogen source. Studies of GCV3 …
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Fall-Kontrollstudie zur Bedeutung von Polymorphismen im MnSOD-Gen für die Sensibilisierung auf para-Phenylendiamin (PPD)
… from cytosine to thymine at position 47 in the mitochondrial targeting sequence in exon 2 results in an amino acid exchange at position -9 in the signal peptide from alanin to valine (Ala-9Val). The same nucleotide exchange at nucleotide position 339 in exon 3 results in an amino acid exchange …
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Functional consequences of cytosine methylation in mitochondrial DNA catalyzed by DNA methyltransferase 1
<p>Cytosine methylation of mitochondrial DNA (mtDNA) was first described several decades ago, but neither the mechanism generating this modification nor its functional significance was known. Because mitochondrial dysfunction is a hallmark characteristic of numerous human diseases, including …
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Strategies for structural studies of poly(ADP-ribose) glycohydrolase: Towards the validation of a novel therapeutic target
… and PARG, has also highlighted the potential of targeting PARG for diseases of inappropriate cell death. A number of obstacles, however, have prevented PARG from being studied as extensively as PARP-1. The extreme sensitivity of PARG to proteases and its insolubility at high concentrations have …
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Molecular cloning and characterization of 3-methylcrotonyl-CoA carboxylase from soybean
… acid residues have the characteristics of a mitochondrial targeting signal peptide. The cleavage site of the signal peptide of the soybean biotin-containing subunit of MCCase has been confirmed by the direct sequencing of the N-terminus of the mature protein;Soybean MCCase has been purified …
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Biochemical, Molecular and Functional Analysis of Volatile Terpene Formation in Arabidopsis Roots
… in mitochondria. We hypothesize that the mitochondrial localization of TPS22 and TPS25 reflects evolutionary plasticity in subcellular compartmentation of TPS proteins with emerging or declining activity. Since (E)-β-farnesene inhibits Arabidopsis root growth in vitro, mitochondrial …
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Characterization of the Helicobacter pylori vacuolating cytotoxin mediated induction of mitochondrial cell death mechanism
… cell mitochondria. VacA intoxication triggers mitochondrial dysfunction and metabolic stress, but the mechanisms underlying these perturbations in mitochondrial function are poorly understood. In this dissertation, we report that infection of gastric epithelial cells with H. pylori results in …
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Regulation of BAX/BAK-Dependent Cell Death Program
… Multidomain proapoptotic BAX/BAK promotes mitochondrial outer membrane permeabilization, whereas anti-apoptotic BCL-2/BCL-X<sub>L</sub>/MCL-1 protects mitochondrial integrity and prevents cytochrome c release. The proapoptotic activity of BAX/BAK is triggered by BH3-only molecules: BH3s) …
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Regulation of the Parkin E3 ligase by PINK1-dependent phosphorylation
… intramolecular interactions. PINK1 is a mitochondrial Ser/Thr kinase that is unique in possessing a N-terminal mitochondrial targeting sequence and three loop insertions within its kinase domain. At the outset of my PhD, PINK1 was discovered to phosphorylate Parkin at Serine 65 …
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Ionic Liquids and GUMBOS for Biomedical and Sensing Applications
… such as luminescence, magnetism, and cancer targeting, were synthesized and explored for use in cancer therapy. In this regard, it is demonstrated that these nanoparticles can not only be used in diagnostics and as drug delivery agents, but also as active pharmacophores. Finally, the third …
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Metal-organic frameworks as intracellular delivery vectors
… systems with high loading capacities and targeting abilities, combining the benefits of both organic and inorganic materials. The following dissertation focuses on developing and evaluating MOFs as intracellular delivery systems. In the first instance, a zirconium-based MOF, UiO-66, was …
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Mitochondrial dysfunction in hereditary optic neuropathies
MITOCHONDRIAL DYSFUNCTION IN HEREDITARY OPTIC NEUROPATHIES Mitochondrial pathologies are a heterogeneous group of clinical manifestations characterized by oxidative phosphorylation impairment. At the beginning of their recognition mitochondrial pathologies were regarded as rare disorders but indeed …
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