Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 87 for “"mitochondrial genome"”.
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Nuclear Encoded Proteins Important in Mitochondrial Genome Stability
… mutations. In addition, loss of Clu1p alters mitochondrial morphology.
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Essentiality of Mitochondrial Genome Integrity in Cell Survival
The mitochondrial genome encodes 22 tRNAs, 2 rRNAs, and 13 polypeptides that are subunits of the electron transport chain involved in oxidative phosphorylation. Because of its proximity to source of ROS generation and its non-chromatinized structure, mitochondrial genome is continuously challenged …
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EXPLORING THE MUTATIONAL LANDSCAPE OF MITOCHONDRIAL GENOME IN HEMATOLOGIC MALIGNANCIES
… apoptosis, and redox signaling, and their genome (mtDNA) is particularly vulnerable to genomic alterations due to its proximity to the respiratory chain, lack of protective histones, and limited repair capacity. Although mtDNA mutations have been implicated in carcinogenesis, their …
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Approaches to mitochondrial genome sequencing using the oxford nanopore minion device
… this study was to develop a reliable method for mitochondrial genome sequencing sans PCR using the Oxford Nanopore MinION. Our hypothesis was that results generated from native DNA sequencing would be concordant to sequencing results generated from PCR-enriched libraries. Error-rates of 1.47% …
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Functional variation in the mitochondrial genome of the yeast saccharomyces cerevisiae
<p>Mitochondrial haplotypes contribute to functional diversity in natural populations. Uniparental inheritance makes it difficult to characterize the genetic architecture of mitochondrially driven phenotypes. In this work I explored the natural diversity of mitochondrial genomes in the yeast <em> …
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Mitochondrial genome engineering in the murine germline using designer nuclease technology
… mitochondrion are encoded in the nuclear genome. However, the mitochondrial genome, which is a circular, multi-copy genome of roughly 17 kb in size, also encodes for 13 polypeptide genes that form key components of the OXPHOS complexes, along with the 22 tRNA genes and 2 rRNA genes …
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Safeguarding the mitochondrial genome: identifying components of the mtDNA repair machinery
Mechanisms that safeguard mitochondrial DNA (mtDNA) are crucial for limiting the accumulation of mutations associated with mitochondrial dysfunction and age-related diseases. Despite this, the pathways responsible for repairing double-strand breaks (DSBs) in animal mitochondria remain poorly …
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A sequencing-based analyisis of epigenetic modifications of the mitochondrial genome
… it regulates gene expression. Methylation of mitochondrial DNA (mtDNA) has been studied using whole genome bisulfite sequencing (WGBS), but recent evidence has uncovered major technical issues, which introduce a potential bias during methylation quantification. In this study, we first validate …
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Analysis of a fragmented mitochondrial genome in a chewing louse, Geomydoecus aurei
1 PDF file (vii, 47 pages)
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How to Mend a Broken Mitochondrial Genome: Mitochondrial Recombination And Its Applications
… and rely on genes encoded within their own genome (mtDNA) in addition to those in the nucleus. mtDNA mutations are linked to mitochondrial and age-related diseases. Therefore, safeguarding mechanisms are crucial to limit mtDNA mutations and curtail ageing and disease. Yet, pathways that …
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Organization and expression of the mitochondrial genome in cytoplasmic revertants ofcms-S maize
… with $\sigma$ and $\psi$ regions of the main mitochondrial genome to generate internal junction sequences of $\sigma$-$\sigma\sp\prime$, $\psi$-$\sigma\sp\prime$, $\sigma$-$\psi\sp\prime$, and $\psi$-$\psi\sp\prime$, thereby circularizing the mitochondrial genome. However, which internal …
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Mitochondrial Genomics: structure, inheritance and phylogenetic utility of the Mitochondrial genome in Bivalvia and Insecta
… interest was centered on the evolution of mitochondrial genome (mtDNA), and on its usefulness as a phylogeographic and phylogenetic marker at different taxonomic levels in different taxa of Metazoa. From a methodological standpoint, my main effort was dedicated to the sequencing of complete …
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Evolution of Nuclear Integrations of the Mitochondrial Genome in Great Apes and their Potential as Molecular Markers
<p>The mitochondrial control region (MCR) has played an important role as a population genetic marker in many taxa but sequencing of complete eukaryotic genomes has revealed that nuclear integrations of mitochondrial DNA (numts) are abundant and widespread across many taxa. If left undetected, …
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Complex evolution of mitochondrial genomes: lessons from salamanders
This dissertation research on genome evolution focuses on understanding the mechanisms that drive the evolution of mitochondrial genome size, content, and organization in animals. This research uses a combination of empirical and computational approaches to examine the evolution of mitochondrial …
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Diachonic DNA Analyses of Animal Breeds and Populations
… limited phylogenetic resolution provided by the mitochondrial genome, the most commonly used ancient DNA marker. In this thesis, I demonstrate that fine-scale diachronic analyses within single animal populations and breeds over short time scales are feasible. First, in order to address the …
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