Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 49 for “"mitochondrial activity"”.
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A toxicological analysis of the cyanotoxin β-methylamino-L-alanine at multiple neural levels and identification of novel modes of activity
… and Ca2+ influx, coupled with disruption to mitochondrial activity and general neuronal death, indicate that the main mode of activity is via excitotoxic mechanisms. Data presented here indicate that BMAA induces increased intracellular Ca2+ influx, DNA damage, mitochondrial activity, lactate …
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Maturation and Culture Media Effects on In Vitro Bovine Embryo Developmental Competence
… content, Mitotracker Red CMX-Rosamine to measure mitochondrial activity, or Cell Rox Green to assess reactive oxygen species (ROS). Using a confocal microscope, images were taken of each stained embryo to detect and measure fluorescence. Any stage 7 embryos that were not imaged were slow frozen …
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Effects of Polydatin on In Vitro Bovine Embryo Developmental Competence, Metabolism, and Cryopreservation
… content or Mitotracker Red CMX-Rosamine for mitochondrial activity or Cell Rox Green for reactive oxygen species. Ten images per embryo were acquired using confocal microscopy at 5-µM step size and detected fluorescence by IMAGE PRO software. Embryos were also slow frozen in ethylene glycol …
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Mitochondrial metabolism and CNS myelination
… of white matter involvement in patients with mitochondrial disorders has been increasing. Given the high-energy demand nature of the brain, this tissue is preferentially affected by energy deficiency caused by mitochondrial dysfunctions. After birth, partial oxygen pressures rise in the …
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Rooibos tea flavonoids increase mineral content in human osteoblast-like cells
… by quantifying alkaline phosphatase (ALP) activity, cell mitochondria activity and toxicity, in addition to changes in regulatory markers of osteoblastic activity. Rutin (≥50μM), hyperoside (≥5.0μM), orientin (0.1μM-1.0μM, 15μM-100μM) and luteolin (5.0μM) enhanced mineral content. This was …
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Developing C. elegans as a model to study Type 2 Diabetes Mellitus
… metabolic rate by partially inhibiting the mitochondrial complex I in mammals. Using C. elegans as a genetic model organism, we show that metformin reduces the mitochondrial activity through endosomal Na+/H+ exchanger, which a previous lab member has found to be a potential target of …
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Regulation of mitochondrial respiration by LDHB suppression in hepatoma cell lines
Increase in aerobic glycolysis and mitochondrial dysfunction are important metabolic features in human cancers. However, it still remains unclear how these phenomena are developed during tumorigenesis. To maintain aerobic glycolysis, continuous generation of NAD+ by lactate dehydrogenase (LDH) with …
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ACETYL COA CARBOXYLASE 1 IN PROSTATE CANCER AS A CHEMOTHERAPEUTIC TARGET
… cancer. Prostate specific deletion of ACC1 activity reduced tumor burden in 12-week-old C57BL/6J mice. However, prostate tumors are able to bypass the requirement for ACC1 by the time mice are 24-weeks of age. Metabolic analysis revealed different prostate cancer cell lines prefer to utilize …
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Mitophagy-Mediated Regulation of ROS Homeostasis During HSC Activation Contributes to Cell Fate
… and HSC exhaustion. Although an increase in mitochondrial activity has been suggested as the cause of HSC depletion in autophagy-defective models, the contribution of mitophagy to HSC function remains to be elucidated. Here, we capitalized on the observation that HSCs lacking the mammalian …
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Investigating metabolic and mitochondrial adaptations triggered by blood flow during the endothelial-to-hematopoietic transition
… Force generated by blood flow stimulates mitochondrial protein translation, cristae formation, increased mitochondrial membrane potential, and oxidative phosphorylation. These adaptations can be mimicked <em>ex vivo </em>by exposing cultured hematopoietic precursors to force, resulting in …
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An examination of psychological stress and its effects on the mitochondrial DNA
… measurement, current findings firmly implicate mitochondrial activity in cell stress reactions. Recent studies have highlighted the effects of psychological stress on mitochondria in terms of oxidative deterioration and crisis signalling. However, these studies have mainly assessed this …
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Exploring the in vitro effects of chronic methamphetamine exposure on selected cancer cell lines
… assessed using the trypan blue exclusion assay. Mitochondrial activity was evaluated using the XTT assay. Intracellular reactive oxygen species (ROS) levels were quantified using the H₂DCFDA assay. All measurements were analysed relative to controls to identify dose- and time-dependent trends. …
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Transcriptional Regulation of Muscle Contractility and Metabolism by a MicroRNA-Mediated Feed Forward Loop
… an increased number of slow myofibers, elevated mitochondrial activity, and exhibit down regulation of the fast myofiber gene program, resulting in enhanced muscular endurance. This effect on skeletal muscle is mediated by the direct binding of Sox6 to conserved cis-regulatory elements upstream …
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The co-modulation of apoptosis and the cell cycle
… with changes in cell volume, total protein and mitochondrial activity indicate that following the initiation of p21\(^C\)\(^I\)\(^P\)\(^1\)-expression cells undergo an increase in their protein synthesis machinery and that the energy, previously required for cell division may be diverted towards …
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A metabolic perturbation by U0126 identifies a role for glutamine in resveratrol-induced cell death
… U0126 acted independently of MEK, inhibiting mitochondrial function and shifting cells to aerobic glycolysis. Mitochondrial activity of U0126 arose through decomposition, producing both mitochondrial fluorescence and cyanide, a known inhibitor of complex IV. Applying U0126 mitochondrial …
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NOVEL INSIGHTS ON ALLAN-HERNDON-DUDLEY SYNDROME: TRANSCRIPTIONAL PROFILING AND FUNCTIONAL CHARACTERIZATION OF THREE GENETIC VARIANTS IN SLC16A2 GENE
… structural components’ impairment, a reduced mitochondrial activity and lipid droplets’ accumulation as a result of a disrupted thyroid hormone signaling. Overall, our study confirms the use of patients’ derived fibroblasts as a reliable model to study in vitro rare conditions, as well as …
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TARGETING TYPE-I PRMTS RESTORES PLATINUM SENSITIVITY IN OVARIAN CARCINOMA THROUGH MITOCHONDRIAL METABOLISM
… post-translational modifications, and mitochondrial metabolic plasticity underpinning chemoresistance in EOC. We present an integrative multi-omics analysis of carboplatin-resistant A2780 ovarian cancer cells (A2780R) developed via stepwise drug adaptation, revealing pronounced …
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The role of apoptosis in <i>in vitro</i> and <i>in vivo</i> models of amyotrophic lateral sclerosis
… In wobbler mice I found that: I) mitochondrial activity is reduced but caspase9 -mediated apoptosis does not seem involved, II) glutamate induced excitotoxicity does not seem involved in motor neuron degeneration, III) markers of neuroinflammation, such as activated microglia and …
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On the discovery and biological characterization of porcine β-Defensin 5 (pBD-5)
… folded disulfide bonds, exhibited antimicrobial activity against Escherichia coli and Staphylococcus epidermidis at physiologically relevant concentrations, underscoring the importance of oxidative folding for its bioactivity. Notably, pBD-5 selectively enhanced mitochondrial activity in B-cells, …
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