Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"misincorporation"”.
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Translational Incorporation of Unsaturated Amino Acids
… and functionality of the translated protein. Misincorporation of unnatural amino acids into this biosynthetic process is rare due to the high fidelity of aminoacyl-tRNA synthetases. This thesis is concerned with the in vitro residue-specific incorporation of unnatural amino acids to produce …
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Translational Incorporation of Unsaturated Amino Acids
… and functionality of the translated protein. Misincorporation of unnatural amino acids into this biosynthetic process is rare due to the high fidelity of aminoacyl-tRNA synthetases. This thesis is concerned with the in vitro residue-specific incorporation of unnatural amino acids to produce …
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The Crosstalk Between DNA Mismatch Repair and Replication
… We show here that consistent with frequent misincorporation by Polɛ-P286R, elevated levels of MMR proteins were found in replicating DNA/chromatin in Polɛ-P286R cells, but this does not result in a reduced mutation frequency, suggesting that cluster binding of MMR proteins at the replication …
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Vergleichende Analyse der Gerstmann-Straeussler-Scheinker-Syndrom-assoziierten Mutation A117V mit der neuen pathogenen Mutation G114V des humanen Prion-Proteins in vivo und in vitro
… within the transmembrane domain of PrP and misincorporation of valine in both cases, PrP G114V and PrP A117V were investigated in comparative studies. Both mutations caused a similar cellular phenotype strongly differing from wild type PrP. Characteristics included reduced expression of …
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Achieving High Replication Fidelity by Intricate Communication between Two Active Sites and Efficient Use of Available Cofactors in Human Mitochondrial DNA Polymerase
… coined the fidelity switch, that detect misincorporation in the nascent base pair and initiate proofreading pathway to maintain high fidelity of mitochondrial DNA. Second, we further probe the fidelity gate on Pol g and its additional role in nucleotide incorporation. Through biochemical, …
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Synthetic approaches to the understanding of DNA nucleobase methylation
… of E. coli DNA polymerase I is examined. The misincorporation frequencies on the ^{7Me9C}dG, ^{7Me7C}dG, and 7CdG templates are comparable to the dG template, although the 7-methyl group slows down the turnover rate of the polymerase when dCTP is incorporated. The stability of ^{7Me9C}dG and …
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Structural And Functional Characterization Of Proteins Of Unknown Function (hp0495, Hp0100 And Hp1259) In Helicobacter Pylori
… for additional mechanisms to prevent the misincorporation of Glu and Asp into proteins in place of Gln and Asn. H. pylori elongation factor (EF-Tu) provides one such machinery, but it is not sufficient to maintain translational accuracy, suggesting the need for additional mechanisms. </p> …
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Development of site-specific RNA labeling strategies to probe alternative RNA splicing
… the University of Leicester revealed significant misincorporation of Z opposite G. Consequently, a change in strategy towards the use of nucleotides developed by the Hirao group was made.Chapter 3 describes the development of a robust synthetic route for the synthesis of a library of …