Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 37 for “"misexpression"”.
-
Gene misexpression in humans
Gene misexpression is the unexpected transcription of a gene in a context where it is usually inactive. In humans, gene misexpression has been implicated in cancers and several rare diseases, for example congenital limb malformations, congenital hyperinsulinism and monogenic severe childhood …
-
The Misexpression of Sonic Hedgehog Leads to Digit Duplication
The limb buds of the early embryo are formed at the lateral flank by an outgrowth of cells from a layer of embryonic tissue called the lateral plate mesoderm. Shortly after this initial outgrowth, cells from the lateral edges of adjacent developing tissue invade the limb bud to form the muscles, …
-
MUSCLE-SPECIFIC MECP2 MISEXPRESSION INDUCES SKELETAL AND VISCERAL MUSCLE DEFECTS RESCUED BY BUTYRATE SUPPLEMENTATION IN DROSOPHILA
… explores the tissue-autonomous effects of MECP2 misexpression in muscle. Aims This project aims to establish a Drosophila model to investigate the impact of MECP2 misexpression in muscle tissue and its role in muscle development, assess whether the resulting phenotypes are autonomous or secondary …
-
EXPRESSION AND MISEXPRESSION OF THE MIR-183 FAMILY IN THE DEVELOPING HEARING ORGAN OF THE CHICKEN
… the chicken inner ear to study expression and misexpression of the miR-183 family. In this study, I reported the differential gene expression of the miR-183 family through development in the embryonic chicken inner ear by in situ hybridization. The spatiotemporal expression patterns of all …
-
Characterization of Codon Optimized Wild Type TDP-43 Mediated Neurodegeneration in a Drosophila Model for ALS.
… robust gain of function phenotypes produced by misexpression of insect codon optimized wild type TDP-43 using the binary GAL4/UAS system, as well as direct promoter fusion constructs with glass promoter. Codon optimized TDP-43 misexpression causes robust phenotypes in the adult eye, wings and …
-
A Genetic Screen for Tribbles Suppressors Identifies the E3 Ubiquitin Ligase Neuralized as a Novel Target
… of the wing and overall smaller tissue size. Co-misexpression of the known targets of Tribbles slbo (Slow Border Cells) and string suppresses the Tribbles wing phenotype. We used this observation as a tool to screen for additional Tribbles targets and from our screen we identified three E3 …
-
Autophagy gridlock in tauopathy
… opposite effect. We also showed that human tau misexpression induced the accumulation of autophagic intermediates which was correlated with a slight increase in macroautophagy induction. The autophagic intermediates were immunoreactive for Blue Cheese, the fly homolog of autophagy-linked FYVE …
-
The Evolutionary Causes and Consequences of Mammalian Hybridization
… associated with sterility. I show that misexpression does not universally tend towards overexpression in sterile hybrids and that misexpression tends to increase with the progression of spermatogenesis. Furthermore, I detected sex-chromosome specific overexpression in both systems but …
-
A DE NOVO COMPUTATIONAL DISCOVERY PLATFORM FROM RNA TO PROTEIN
… muscular dystrophy (FSHD). Our results show that misexpression of DUX4, which encodes an embryonic transcription factor, impairs RNA metabolism by inhibiting Nonsense-Mediated Decay, thus leading to the accumulation of incomplete transcripts and truncated proteins. De novo transcriptome assembly …
-
Altered FRG1 Levels During Xenopus Laevis Development Leads to Muscular and Vascular Phenotypes Supporting a Role for the Misregulation of FRG1 in FSHD
… region gene 1) is a leading candidate gene whose misexpression may lead to FSHD. As FSHD pathology is most prominent in the musculature, most research and therapy efforts have focused on muscle cells. However, between 50-75% of FSHD patients also exhibit retinal vasculopathy and FSHD muscle has …
-
The Role of Wnt Signaling in Development of the Ophthalmic Trigeminal Placode.
… specific molecular marker of opV placode cells. Misexpression of dominant-active β-catenin as an activator of canonical Wnt signaling, however, is not sufficient to promote the opV placode cell fate. We conclude that canonical Wnt signaling is necessary for normal opV placode development, and …
-
The Evolution of Drosophila Immunity
… was mostly cis-driven, and there was extensive misexpression of immune-related genes in hybrids.
-
Role of the paired-like gene Pitx1 in Xenopus head development.
… mesoderm, and first branchial arch derivatives. Misexpression of xPitx1 in whole embryos leads to the formation of enlarged or ectopic cement glands. In addition, variable posterior deficits are observed with extreme cases where the embryo exhibits no recognizable structures posterior to the …
-
VALIDATION OF CANDIDATE GENES IN RESPONSE TO VERSICAN MANIPULATION IN DEVELOPING SYNOVIAL JOINTS
… and Wnt pathway genes as they pertain to the misexpression of versican. RT-PCR and real-time PCR were used in this study to validate expression of the genes chosen. Through the use of these techniques the degree of expression has been quantified and compared to the fold changes observed with …
-
Middle ear development: Genetics and disease
… of joint development and show<br/>Gdf5 misexpression in the Eya1 +/- middle ear. I suggest Eya1 indirectly regulates middle ear joint patterning through a more general role in cartilage development. During postnatal development, Eya1 may further be required for the maintenance of joints. …
-
Mechanisms of Type IV Collagen Targeting to Developing Basement Membranes
… endogenous localization, conditional knockdown, misexpression, and RNAi screening techniques to investigate how the sole C. elegans type IV collagen molecule is recruited to the BMs of growing gonadal and pharyngeal organs during larval development. In Chapter 1, I review BM structure and …
-
Growth Cone Pathfinding and Neuromuscular Synaptogenesis in Drosophila Midline Mutants
… null muscles restores motoneuron synaptogenesis. Misexpression of a cytoplasmically truncated commissureless construct in muscles functions as a dominant negative by inhibiting internalization and synaptogenesis. My findings support a role for commissureless modifying the molecular profile of the …
-
The Roles of the Drosophila Protein Tribbles in Oogenesis and Insulin Signaling Pathway
… Trbl level required for BC migration. In a wing misexpression screen for Trbl interacting proteins, I identified the Serine/Threonine protein kinase Akt, a major mediator of insulin signaling. In recent years, mammalian Trib3 and Trib2 proteins have been implicated in the regulation of Insulin …
-
The Polycomb Complex 2 contributes to developmental delay when histone methylation is inappropriately inherited
… that H3K27me contributes to MES-4 germline gene misexpression in the somatic tissues of <em>spr-5; met-2</em> mutants. We completed RNA-seq, ChIP-seq and qRT-PCR experiments to further examine how these histone modifying enzymes cooperate to regulate proper germline versus somatic gene …
-
WHO: A Novel Wuschel-Like Homeodomain Transcription Factor in Arabidopsis
… Taken together, these findings indicate that the misexpression of WHO caused by promoter element disruption affect fundamental developmental programs in Arabidopsis, and suggest that the homeobox gene WHO plays an important role in regulating meristematic cell differentiation and organ formation.
Page 1 of 2