Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"microwells"”.
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The Influence of Human Umbilical Vein Endothelial Cells in the Formation of Glioblastoma Spheroids in Three-Dimensional Microwells
… GBM cells with endothelial cells in the hydrogel microwells would mimic the in vivo microenvironment of tumor cells. Thus, we could provide beneficial 3D in vitro co-culture models for GBM treatment with oncolytic adenovirus, Delta-24-RGD (Δ24RGD). Therefore, in the first part of this study, we …
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Microengineered responsive platforms for spatial and geometrical control of multicellular organizations
… produce homotypic cell-cell interactions. Microwells fabricated from poly(Nisopropylacrylamide) (PNIPAAm) responded to temperature by changing their shapes. Spherical microtissues of a single cell type were formed in responsive microwells and recovered by using shape changing properties of …
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Improving the development of bovine in vitro produced embryos cultured individually
… were either cultured in standard droplets or microwells made at the bottom of culture droplets either in groups or individually for a 2x2 design. This experiment experienced poor development in all treatments including the group control, and none of the treatments were found to be …
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Design of a Portable Device to Detect Per- and Polyfluoroalkyl Substances (PFAS) in Water
… fluids into a cartridge of many single use microwells. The microwells are multilayered devices designed to not interact with PFAS that can be easily integrated together into a cartridge with more than a hundred individual microwells fitting in a single 25 X 25 mm2 sheet allowing many tests …
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Integrated Microfluidic System for Digital Detection of Extracellular Vesicles
… and a smart actuation device for sealing the microwells. The chip achieves the EV quantification by digital Enzyme-Linked Immunosorbent Assay (ELISA) and enzymatic signal amplification. Specific areas of glass slide beneath the detection area of the chip coated with anti-CD81 antibodies are …
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Design and fabrication of microfluidic valves using poly(N-isopropylacrylamide)
… The design of the valve consists of filling microwells with poly(NIPAAm) and bonding PDMS channels above them. This filling is achieved using thermal inkjet printing of a prepolymer solution and subsequent polymerization using UV irradiation. When the gel is swollen, it blocks flow from …
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IN VITRO MODULATION OF HUMAN CORNEAL ENDOTHELIAL CELL BEHAVIOR VIA TOPOGRAPHICAL AND BIOCHEMICAL CUES
… The topographies that we test are nanopillars, microwells, and micropillars. The biochemical cues are extracellular matrix coating of fibronectin-collagen I, FNC Coating Mix, and laminin-chondroitin sulfate. Our results indicate that topographical and biochemical cues are able to alter HCEC …
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Determination of Pancreatic and Salivary Amylase By Enzyme Immunoassay and Their Prevalence in Hyperamylasemic Patients
… of the p-amylase and s-amylase was developed. Microwells were utilized for the solid-phase immobilization of the amylase inhibitor cycloheptaamylose (CHA) which was coupled to bovine serum albumin (BSA) by carbodiimide coupling. Monoclonal antibodies were produced against p-amylase and …
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Microfluidic Assessment of Stem Cell-Derived Pancreatic Beta Cells
… device from photolithography, containing microwells capable of immobilizing human β islet or stem cell-derived β cells for perifusional analysis with low (2mM) and high (20mM) concentrations of glucose, following subsequent dynamic estimation of insulin produced using the insulin ELISA. …
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Development of High-Throughput Platforms for Single-Cell Analysis
… tools that rely on microfabricated arrays of microwells. The first platform merges fluorescence cytometry with label-free profiling of the small molecule composition of tens of thousands of cells based on matrix assisted laser desorption/ionization (MALDI) mass spectrometry. We evaluated …
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Analysis of DNA damage and repair responses in cerium exposed cells and hepatocyte spheroids
… the agarose chip contains a grid of ~100 microwells condensed into the area of a single well of a standard 96-well plate. HepG2 spheroids formed in these microwells through agarose assisted aggregation, and cell viability was maintained over time. To test efficacy for genotoxicity studies …
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Microbead-Based Biosensing in Microfluidic Devices
… technique was used to stamp an array of microwells in a thin plastic substrate where appropriately functionalized agarose microbeads were selectively placed within a conduit. Functionalized quantum dot nanoparticles were pumped through the conduit and used as a fluorescent label to …
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Microfluidic devices for studying early response of cytokine signaling
… experiments and manual assays by standard microwells and pipetting techniques. The developed method also provides high time-resolution and reproducible data for studies of cell signaling events, especially at early stages. These cell signaling events are difficult to be investigated by …
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Patterned substrates for cell culture with electrohydrodynamic jet printing
… mixture to create substrates with circular microwells. Finite element modeling (FEM) was used to show that microwell topography was a result of backfill contraction during exposure. The FE model was then used to make predictions for further substrate design. The techniques presented in this …
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Inexpensive technologies enabling widespread utilization of image-predicated cell sorting
… allowed to settle into an array of cell-sized microwells. A laser is then used to optically levitate desired cells out of the array and into a flow stream for collection. This thesis demonstrates > 100-fold enrichment of target cell populations and up to 89% output purity following a single …
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Design and testing of a high accuracy robotic single-cell manipulator
… be able to pick individual cells from array of microwells, each 30 Pm or 50 pm cubed. Design efforts have been made for higher accuracy, higher throughput, and compactness. The proposed system is designed to have a T-drive mechanism with two linear stages for XY-plane positioning to have higher …
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Competitive Enzyme Immunoassay by Polyclonal Antibodies Against Organophosphorus Pesticides
… instrumentation required to test OPs.</p> <p>Microwells and microtubes were utilized as the solid-phase immobilization for the development of an enzyme immunoassay for OPs. A sensitive and simple microtube based competition enzyme immunoassay for the quantitative determination of …
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Encoded hydrogel microparticles for high-throughput molecular diagnostics and personalized medicine
… and gel particles in polydimethylsiloxane (PDMS) microwells, it was possible to dramatically improve the particles' target capture efficiency and thereby move closer to a regime in which miRNAs and other biological molecules may be directly detected without target amplification from single cells.
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Microfluidic processes to create structured microparticle arrangements and their applications
… precision, specificity, and versatility). Microwells are fabricated on top of porous membrane by a vacuum-assisted molding method. Particles are guided and assembled into wells by hydrodynamic force associated with fluid flow through pores in microwell. Iteration of assembly and washing …
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Investigating the role of geometry, mechanics, and microenvironmental cues on liver progenitor cell differentiation
Engineered systems permit investigation of the variety of signals in the microenvironment that direct cells and tissue fates. This work focuses on developing and using such systems to investigate how mechanical characteristics impact liver progenitor cell differentiation. In this dissertation, I …