Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"microvesicle"”.
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Biochemical and physiological analysis of microvesicle subtypes
… of extracellular vesicles: in particular microvesicles (MVs). The characterisation utilised novel applications of existing protocols, demonstrated for the first time within this thesis, resulting in an up-to-date and greatly improved series of techniques for the analysis of microvesicles. …
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Light Scattering Measurement of Phospholipid Microvesicle Membrane Fusion Kinetics
The moments of the time constant density function, as measured by quasi-elastic light scattering and Koppel's method of cumulants, are expressed in terms of moments of the size density function of single-walled hollow spherical scatterers. An equation governing the evolution of fusing particles is …
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Expression and secretion of basigin by human placental trophoblast cells occurs via microvesicle shedding and is a regulated process
… surface of trophoblast-like cells occurs through microvesicle shedding; 4) the signaling cascade responsible for microvesicle release is the protein kinase C (PKC) pathway; 5) BSG-containing microvesicle release by trophoblast-like cells is altered in response to hypoxia/reoxygenation injury; 6) …
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Development of novel isolation methodologies for microvesicles and exosomes, as potential biomarkers for health (Therapeutic Lifestyle Changes), and use of microvesicle-delivered β-Gly in erythroleukaemia
Both MVs and exosomes are isolated by similar methodologies such as differential centrifugation of cell culture supernatant (S/N), filtration, floating on chemical gradients or using antibody labelled magnetic beads. Hence, the isolation techniques, commonly in use, provide low levels of …
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HOW MICROVESICLES ARE "BUDDING" INTO THE CANCER CONVERSATION: THE ROLE OF GLYCOCALYX-INDUCED MEMBRANE BENDING
… contribute to tumor progression and metastases. Microvesicles, a specific EV subtype, directly shed from the plasma membrane, but how this occurs is not fully elucidated. To help address this, there is a need for consistent convention and methodology to clearly differentiate and fully …
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The Influence of Membrane Lipid Order on Cell Shape and Microvesiculation in Human Erythrocytes
… shedding of the cell membrane in the form of microvesicles. We hypothesized that both the shape transition and microvesiculation are influenced by microscopic membrane physical properties such as lipid order. To test this hypothesis, membrane properties were manipulated by varying the …
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A study of bioprocess designs for development of the myogenic secretome as a source of bioactive extracellular vesicles
… nm) were isolated at higher concentrations than microvesicle particles. Chondrocyte internalisation of 2D and 3D myogenic EVs possibly via phagocytosis, displayed heterogeneous EV distribution. The myogenic secretome may enhance collagen II expression and reduce NF-kB expression in chondrocytes. …
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Identification and characterisation of micrornas involved in the pathogenesis of HIV–associated non-Hodgkin's lymphoma
… line Ramos that were exposed to HIV compared to microvesicle treated cells. It was initially planned to use both normal B-cells (L1439A) and BL cells for analysis but Ramos was selected due to technical reasons for this step. Thereafter we validated selected miRNAs by quantitative real-time PCR …
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HIV alters the expression of miRNA hsa-miR-200c-3p in B-cells, leading to enhanced migration of lymphoma cells
… and BL41) exposed to HIV-1 compared to matched-microvesicle treated (control) cells. Following validation, the role of miRNA hsa-miR-200c-3p in the development of HIV-associated BL was investigated. This was done by using online bioinformatic prediction tools, as well as literature searches, to …
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Mechanistic studies on the role of extracellular vesicles in the tumor microenvironment
The transition from normal into malignant cells with acquired uncontrolled growth is a process where cancer cells viciously develop adaptive strategies to overcome microenvironmental and metabolic stress. Hypoxia, or deficient oxygen supply, is a common feature of the expanding tumor which alters …