Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"microtubule network"”.
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Regulation Of The Tubulin Code In Cardiac Hypertrophy And Failure
A proliferated and post-translationally modified microtubule network underlies cellular growth in cardiac hypertrophy and contributes to contractile dysfunction in heart failure. Yet how the heart achieves this modified network is poorly understood. Determining how the “tubulin code” – the …
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Modelling and image processing of microtubule dynamics and organisation
Microtubules, dynamic protein polymers, form networks that are essential for intracellular organisation. Involved in many cellular processes that are vital in development and homeostasis, improper regulation of the microtubule network is implicated in various diseases. This work addresses the …
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MES-DERIVED CARDIOMYOCYTES AS CELLULAR MODEL TO STUDY CARDIAC DISEASES: ALTERATIONS IN STRN AND GNB5 GENES
… of STRN was reported to destabilize microtubules and increase INa, immunofluorescence analysis confirmed the higher Na+ channel expression and a more dynamic microtubule network in KO CMs. Motion video tracking analysis highlighted an altered contraction in STRN-KO CMs, and this was …
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Bidirectional transport by molecular motors
… motors which transport various cargos along microtubule filaments. The microtubules possess an intrinsic direction, having a 'plus' and a 'minus' end. Some molecular motors such as cytoplasmic dynein walk to the minus end, while others such as conventional kinesin walk to the plus end. Cells …
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Dual Roles for NBR1 in Bone Remodelling and Autophagosomal Protein Degradation
… a direct interaction between Nbrl and the microtubule associated protein MAP IB. It is hypothesised that via its interaction with MAP IB and LC3, Nbrl targets ubiquitinated proteins to the microtubule network where they can be transported to sites of autophagosomal formation. Further …
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Role of Syk in the regulation of cytoskeleton and stress granules in breast cancer
… and allowed visualization of a more substantial microtubule network. The microtubules of Syk-expressing cells were more stable to nocodazole-induced depolymerization and were more highly acetylated than those of Syk-deficient cells. Silencing of MAP1B, a major substrate for Syk in MDA-MB-231 …
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Morphogenesis of follicular epithelium in Drosophila melanogaster: function of von Hippel-Lindau tumour suppressor gene
… I determined that d-VHL binds to and stabilizes microtubules. Loss of d-VHL depolymerizes the microtubule network during oogenesis, leading to a possible deregulation in the subcellular trafficking transport of polarity markers from Golgi apparatus to the different domains in which follicle cells …
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Relationship between Hepatitis B Virus Core Particles and Intracellular Motility
… Mitochondria were stained by MitoTracker. And microtubules (MTs) was visualized using anti-tubulin antibody. RESULTS: The mitochondrial aggregation towards the nucleus was observed in HBV replicating stable cells, but not in HuH7 and HepG2 cells. When HBV replicating stable cells were treated …
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Rab8 Directs Tubulation and Furrow Ingression During Epithelial Formation in Drosophila Melanogaster
… an epithelial sheet. Additionally, an intact microtubule network is required for proper Rab8 dynamics during early furrow formation, but is dispensable once a Rab8 cortical array has formed. These studies suggest that active, membrane-bound Rab8 populations prefigure and initiate furrow …
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Chlorpyrifos and chlorpyrifos oxon induce neurite retraction and cytoskeletal disruption in mouse N2a cells and human neural progenitor stem cells
… (pNFH) monoclonal antibody SMI34, while the microtubule network was unaffected. Western blotting analysis revealed transiently increased levels of reactivity of Ta51 after 2 h exposure but reduced levels of reactivity following 8 h treatment with both compounds, whereas reactivity of …
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A biochemical and molecular analysis of functional differences between dystrophin and utrophin
… is not sufficient to rescue the disorganized microtubule network of the mdx mouse. Thus, we have elucidated a second functional difference between dystrophin and utrophin. Additionally, Fiona-mdx mice lack full recovery of cage activity after mild exercise. Our results suggest that any …
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Investigating Mechanotransduction and Mechanosensitivity in Mammalian Cells
… motor proteins form a contractile (actomyosin) network inside the cell that is connected to the extracellular microenvironment through focal adhesion and integrin sites. The transmission of internal actomyosin strain to the microenvironment via focal adhesion sites generates mechanical traction …
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In vitro reconstitution of Central Spindle Motility
… dedicated motor proteins, to cytoskeletal network. While much is known about the biophysics of motors on isolated tracks, how the complex cytoskeleton topologies found in cells, such as antiparallel microtubule (MT) overlaps, can influence the steady state distribution of cargoes is not …
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Collective dynamics of astrocyte and cytoskeletal systems
… study collective behavior in cellular networks with unprecedented detail. Imaging the electrical signaling of neuronal networks at the cellular level has generated exciting insights into the multiscale interactions within the brain. This thesis aims at a complementary view of the …
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Nanolithography and nanoscopy methods for the study of biological samples in confined spaces
… which are then transported along the cellular microtubule network. Thirdly, STED is used to image the morphology of the endoplasmic reticulum (ER) in primary neurons. Calnexin is a chaperone-like membrane protein on the ER surface which is due to its close connection to glucoglycans of great …
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ANALYSIS OF HERPES SIMPLEX VIRUS ICP0 FUNCTION AND THE ANTIBODY RESPONSE TO A LIVE HSV-2 ICP0- MUTANT VACCINE
… to the cytoplasm where it reorganizes the host microtubule network (PLoS ONE 5(6): e10975). In the first part of my dissertation, we used the anti-neoplastic drug paclitaxel to determine if ICP0’s microtubule-reorganizing activity is required for efficient replication of HSV- 1. Surprisingly, we …
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Regulation of Tubulin dynamics by the +Tip tracking protein Mal3
The Microtubule (MT) network is a central component of the eukaryotic cell cytoskeleton. In the fission yeast S. pombe, a complex of three proteins specifically tracks MT +ends and stabilizes MTs in the cell. It is composed of the proteins Mal3, Tip1 and Tea2. Mal3, the S. pombe homologue of EB1, …