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Showing 1 to 20 of 20 for “"microtiter plate"”.
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The Use of Natural Products as Potential Anti-Pseudomonas Agents
… evaluated at various concentrations by using a microtiter plate assay or culture tube assay. The inhibition of biofilm formation was also determined using crystal violet assay. The results indicated that the selected natural products have antibacterial effect and anti-biofilm formation on …
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Hodnocení efektivity vybraných metodických přístupů za účelem disagregace stafylokokové biofilmové biomasy
… methodical configurations - in the wells of a microtiter plate and on pegs (analogy to the Calgary Biofilm Device). To evaluate the degree of consistency of the obtained data, which reflects the homogenity of the biofilm biomass disaggeragation, the "recovery" method, and the method of …
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Development of a rapid riboflavin growth-based assay using Lactobacillus rhamnosus
… the food industry. By scaling down the assay to microtiter plates both, time and materials can be conserved. Use of microtiter plates would also allow for numerous samples to be assayed simultaneously. The goal for developing the microtiter plate assay is to obtain results more rapidly while …
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Solid-supported synthesis of peptidoglycan fragments and their biological evaluation
… different PBPs were studied and a protocol for microtiter plate-based assays is developed allowing the screening of compound libraries for inhibitors of PBPs. In the future, the synthesized peptidoglycan fragments will be used as reference compounds in the framework of the NetRBI project.
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Directed evolution of a subtilisin Carlsberg variant towards improved perhydrolytic activity for industrial application
… was optimized for screening in 96 and 384-well microtiter plate system, with the possibility to further upscale throughput by using flow cytometry screening technology. The screening platform was developed in this work to improve protease and likely other hydrolases (e.g. lipases or esterases) …
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Effects of Cinnamon Water Extract as a Cariostatic Agent on Nicotine-Induced Streptococcus Mutans Biofilm
… Then a 24-hour culture of S. mutans UA159 in microtiter plates was treated with varying nicotine concentrations (0-32 mg/ml) in TSBS at the same time with or without the optimum cinnamon water extract concentration. A spectrophotometer was used to determine total growth absorbance and …
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New phosphorescence based probes and techniques for the analysis of cellular oxygen and respiration
… and two-fold higher sensitivity to compare with microtiter plate. We compared three common OCR platforms: hermetically sealed quartz cuvettes for absolute OCRs, partially sealed with mineral oil 96-WPs for relative OCRs, and open 96-WPs for local cell oxygenation. Both 96-WP platforms were …
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The Elimination of Listeria monocytogenes Biofilms from Stainless Steel Deli Meat Slicer Components by the use of Hurdle Technologies
… innocua by observation through motility tests, microtiter plate biofilm assay and microscopy. Listeria strains were grown on stainless steel coupons cut from a deli meat slicer blade in order the observe biofilm growth. This study also investigated the synergistic effects of steam and chemical …
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The Antimicrobial Activity of Cold Pressed Terpeneless Valencia Orange Oil at Cold Temperatures
… The strains were tested using a ninety-six well microtiter plate method, with or without membrane filtration. The compound 2, 3, 5-triphenyl tetrazolium chloride was used as a growth indicator. Serial, two fold dilutions of the oil were tested. Plates were incubated at 37, 10, or 4°C for times up …
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Discovery and Characterization of Small Molecule Inhibitors of Biofilm Formation in Pseudomonas Aeruginosa
… iron chelators as iron delivery agents in a microtiter plate assay for biofilm production. Picolinic acid and acetohydroxamic acid-chelated iron were able to stymie biofilm production at moderate concentrations in P. aeruginosa PA14 and 17 Pseudomonas strains from CF patients. Chelated iron …
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Synthesis, Characterization, Critical Micelle Concentration and Antimicrobial Activity of Two-headed Amphiphiles
… 2CCbn series amphiphiles were measured by using microtiter plate reader and by looking turbidity. The cutoff effect was found for the 2CCbn series. The MIC decreased up to C20 chain length and started rising for C22. The 2CCb18 (MICâ 2.2 µg/mL) of the 2CCbn series was the most effective …
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Triazole-linked reduced amide isosteres: An approach for the fragment-based drug discovery of anti-Alzheimer's BACE1 inhibitors and NH-assisted Fürst-Plattner opening of cyclohexene oxides
… inhibitor project we used an originally designed microtiter plate-based screening to discover 4 triazole-linked reduced amide isosteres that showed modest (single digit micromolar) BACE1 inhibition. Our ligands were designed based on a very potent (single digit nanomolar) isopththalamide ligand …
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Online monitoring in continuously shaken microtiter plates for scalable upstream bioprocessing
This thesis is focusing on the applicability of microtiter plates as platform for high-throughput experimentation in bioprocess development. Therefore, three main aspects have been studied in this thesis: 1) characterization of mass transfer in microtiter plates (MTPs), 2) development of a new …
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PCR-Slide: a modular and cascadable platform for DNA sample processing with integrated nanoliter dosage
… pitch corresponding to the pitch of the 384 well microtiter plates (4.5 mm). This allows the transfer of sample liquid into the wells of such a microtiter plate. As the reaction cavities of the PCR-Slide are also arranged in the same way, it is possible to dispense from one PCR-Slide into another …
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Selektive Markierung von Glykokonjugaten mit modifizierten Donorzuckern als Substrate von Glykosyltransferasen und Glykosidasen
… simple detection method by the sandwich ELSA in microtiter plate format and the high level of biotinylation rise high expectations in the direction of an early diagnosis of rheumatoid arthritis. With pNP-6-biotinyl-Gal(NAc) potential substrates of the selective transfer with glycosidases have …
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Entwicklung einer modellbasierten Hochdurchsatztechnik zur Enzymcharakterisierung mit Schwerpunkt auf Langzeitstabilität
… consists of short term enzyme tests in 96-well microtiter plates under partly extreme conditions to predict the enzyme long term stability under moderate conditions. The technique is based on the mathematical modeling of temperature dependent enzyme activation and deactivation. Adapting the …
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The detection of occult metastatic disease in patients with cutaneous melanoma
… valuable and were used to develop a novel microtiter plate assay for measuring the active fraction of PAI1 in biological fluids by making use of Mabs against different epitopes of PAI1.
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Novel Determinants That Influence Azole Susceptibility in Candida glabrata and Candida albicans
… associated with trailing growth in a liquid microtiter plate assay. We also demonstrate that an Rta3p-GFP fusion protein localizes predominantly to the plasma membrane, consistent with a putative function for Rta3p as a lipid translocase.</p>
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Defining the mechanisms of microbial sensing among members of the toll-like receptor 2 sub-family
… for lipopeptides and the TLR2 co-receptor. Using microtiter plate assays, we have demonstrated that Pam3CSK4 induces the formation of the TLR1/TLR2 and TLR2/TLR10 heterodimeric complexes, and have further shown that ternary complex formation is ligand-specific and can be blocked by monoclonal …
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High-throughput experimentation for microscale cultivations and recombinant protein expression
… common high-throughput cultivation processes in microtiter plates are investigated with the BioLector technology. It is demonstrated that extensive monitoring of key parameters (i.e. biomass and product formation) in microscale cultivations enables a profound understanding and hence optimization …