Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"microtissues"”.
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Positioning Microtissues on the Differentiation Continuum Reveals their Utility for Studying Skeletal Myopathies and Metabolic Disease
… insulin resistance. Human skeletal muscle microtissues, differentiated under uniaxial tension across two microposts, exhibit greater insulin responsiveness than 2D myotubes and are amenable to contractile stimulation. However, a microtissue-based model of T2D has not been developed. The …
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Development of a micromixing system for treatment of ORL 48 microtissues in different concentrations of cytochalasin B
… of CB-DMEM were applied on to ORL-48 microtissues produced by using flicking technique. The morphological responses, cell viability and cell proliferation of ORL-48 monolayer cells (2D) and microtissues (3D) treated in four different CB concentrations were assessed via phase contrast …
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Development of an electronic aerosol atomisation system for generating three-dimensional (3D) cells in microencapsulations and microtissues characterisation
… controller circuits. The microcapsules and 3D microtissues were biophysically characterised. For the output of the system, the microcapsules size slightly increased with the extrusion rates and decreased significantly with the airflow rates. At an extrusion rate of 20 μl/min and airflow rate of …
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Detection of chemical nutrients in water using a fluidic microplasma chip with enhanced optical collection efficiency
… microfluidic droplet device for fabricating 3D microtissues and studying the cell behaviors in 3D microtissues. It has been found by properly selecting the size of the microchambers on the microfluidic device and choosing an optimal concentration of collagen to fabricate microtissues. The …
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Engineered microtissue platforms for modeling human pathophysiology and drug metabolism
… populations of miniaturized 3D tissues, termed 'microtissues,' for modeling healthy and diseased tissues in both static and perfused systems. First, we developed a flow-based platform to test tumor proliferation in defined microenvironmental settings with large numbers of replicates (n > 1000). A …
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Establishing species-specific 3D liver microtissues for repeat dose toxicology and advancing in vitro to in vivo translation through computational modelling
… in the production of viable and reproducible microtissues, amenable for high-throughput investigations. I show that our in vitro system mimics the in vivo cellular morphology, exhibiting both structural and functional polarisation, along with active and functional transporters. Chapter 3 …
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Microengineered responsive platforms for spatial and geometrical control of multicellular organizations
… methods to fabricate spherical and stripe microtissues of single cell types and their temperature-controlled retrieval. These microtissues were scaffold-free and can potentially produce homotypic cell-cell interactions. Microwells fabricated from poly(Nisopropylacrylamide) (PNIPAAm) …
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Deep and Dynamic Metabolic and Structural Imaging in Living Tissues
… 700µm in living engineered human multicellular microtissues by adopting multimode fiber (MMF)-based low-repetition-rate high-peak-power three-photon (3P) excitation of NAD(P)H at 1100nm. This is achieved by having over 0.5MW peak power at the band of 1100±25nm through adaptively modulating …
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Investigating the role of geometry, mechanics, and microenvironmental cues on liver progenitor cell differentiation
… of 3D, multicellular liver progenitor cell microtissues in constrained geometries. These generated distinct mechanical profiles which I modeled with finite element method software, which was supported with mechanical measurements. I used this system to correlate patterns of tensile and …
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Investigation and control of dermal fibroblast signaling during injury repair
… imaging and manipulation of calcium in 3D microtissues, the development of a reporter for smooth muscle actin and a labeled cellular fibronectin fusion protein, and the optimization of Forster Resonance Energy Transfer sensors. Taken together, our experimental results demonstrate the …
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Synthesis and Applications of Large-Area Monolayer Graphene
… demonstrating its use as a sensor for cardiac microtissues, and as a tunable microheater in mid-infrared devices. Altogether, this work advances both the fundamental understanding and technological scalability of monolayer graphene, positioning it as a versatile platform for future applications …
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MULTISCALE TECHNOLOGIES FOR ENGINEERING AND CRYOPRESERVING OVARIAN TISSUES AND HUMAN IPSCs
… and (2) cryopreservation of the cells/microtissues. To understand the microenvironment of the ovary for informed tissue engineering system design, we spatially characterize the micromechanical properties of ovarian tissue from domestic cats to reveal both elastic and viscoelastic …
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ENGINEERING STEM CELLS AND ORGANOIDS ON A CHIP FOR THE STUDY OF HUMAN HEALTH AND DISEASE
… microvascular beds and vascularized 3D human microtissues and ii) blood-retina barrier of the human retina. Using our vascular engineering strategy, we further demonstrate advanced capabilities of our microengineered models to incorporate patient-derived induced pluripotent stem cells to …
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Microscale Tissue Engineering and Contributors of the Cellular Niche
… transplant and produce at large scale. Our RPE microtissues were designed to capture the benefit of both approaches: namely, simplicity of production and transplantation, as well as enhanced performance. We found that our RPE microtissue exhibited superior cellular behaviour in terms of gene …
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Development of a novel co-culture based in vitro model system to study the wound healing process
… cells and L8 myoblast cells formed 3-D microtissues in vitro that expressed MyoD, P4H, and á-smooth muscle actin. The micromass tissue layer remained adherent to the culture plate when inflicted with a single laceration injury, which allowed monitoring of cell migration into the wound …