Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 12 of 12 for “"microsomal membranes"”.
-
Early Events in the Biosynthesis and Subcellular Consignment of Secretory and Membrane Proteins
… systems supplemented with heterologous microsomal membranes either during or after translation: a) proteolytic enzymes, SDS-PAGE and autoradiography have been used to characterize various biosynthetic forms of bovine pituitary growth hormone and prolactin, vesicular stomatitis virus …
-
Studies on the Degradation of Dihydrofolate Reductase and Other Intracellular Proteins by Mammalian Cells
… volume of the proteins of the subcellular membranes, this material probably represented enzyme which was inactive and bound to these membranes. Greatest binding occurred with membranes of the microsomal fraction.</p> <p>The formation of inactive enzyme of high molecular weight did not …
-
Parallel effects of signal peptide hydrophobic core modifications on cotranslational translocation and posttranslational cleavage by purified signal peptidase
… and processing of the mutant signal peptide by microsomal membranes. Decreasing the length of the core by 6 residues abolished signal activity and partially blocked its interaction with signal recognition particle. Deleting only 2 residues from the core resulted in a position dependent effect on …
-
Poliovirus Replication: Interactions with Cytoplasmic Membranes and with Compounds Which Affect Virus Biosynthesis
… is intimately associated with intracellular membranes and affects their production and distribution within the cell. We have shown that the density distribution of cytoplasmic membranes separated by isopycnic centrifugation in discontinuous sucrose gradients is progressively altered after …
-
Insertion Signal and Membrane Anchor Functions of the Amino Terminus of Cytochrome P45011C2
… protein was not translocated to the interior of microsomal membrane vesicles. Full length P450 proteins integrated into microsomal membranes in an SRP dependent manner, while partial proteins lacking the amino terminus failed to integrate. P450 transcripts produced more than one translation …
-
Identification and characterization of a novel structural protein of porcine reproductive and respiratory syndrome virus, the replicase nonstructural protein 2
… nsp2 was found to strongly associate with canine microsomal membranes. Through high-speed ultracentrifugation, protease protection assay, and immunoprecipitation nsp2 was defined as an integral membrane protein and additionally identified to display an unexpected N-terminal cytoplasmic / …
-
Membrane Domain of Plant 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase: Targeting, Topology, and Function
… reveal that tomato HMGRs are targeted to microsomal membranes in a cotranslational fashion that does not involve cleavage of an N-terminal targeting peptide. Membrane topography of HMGR was revealed by protease protection studies, indicating that both tomato HMGRs span the membrane two …
-
Development of a Cytochrome P450 Expression System with Biomass Scale-up, Streamlined Downstream Processing and Stability Modifications
… These ER-bound P450s are usually referred to as microsomal P450s. A yeast expression system was used to co-express several human P450s and their reductases, essential for P450 activities. Episomal and chromosomally integrating plasmids with auxotrophic markers were used to build the system. Gene …
-
Post-translational Insertion of a Small Tail-Anchored Protein into the Membrane of the Endoplasmic Reticulum
… vitro translation system supplemented with rough microsomal membranes (RM) post-translationally. Upon insertion into the membrane, RAMP4op becomes N-glycosylated. This allows clear discrimination between cytosolic and membrane inserted forms of the protein. In this assay system, RAMP4op can be …
-
The Golgi Complex of the Hepatocyte: Isolation and Partial Enzomological Characterization
… press). After this treatment, a fraction of membranes largely free of lipoprotein particles could be isolated by centrifugation. This fraction is assumed to represent the membranes which bounded the Golgi vesicles in the cell. The total Golgi fraction (membrane and contents) and the isolated …
-
Crosstalk of protein phosphorylation and lysine acetylation on 14-3-3 proteins and their applications in brassinosteroid signaling and photosynthesis
… greater in the 14-3-3ω that was associated with microsomal membranes compared to the soluble phase. 14-3-3ω was co-immunoprecipitated with the BRI1-BAK1 complex in vivo, and BRI1-bound 14-3-3ω was phosphorylated at Thr-94 and Thr-140 sites. Moreover, the phosphorylation of both sites responded to …
-
Organismi acquatici e ambiente: meccanismi biochimici di interazione, risposta e adattamento
… ATPase activities of plasma- and mitochondrial membranes from tissues of cultured marine bivalve molluscs and potentially stressful environmental conditions, such as the exposure to contaminants both of natural origin (ammonia nitrogen, the main contaminant of aquaculture plants) and of …