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Showing 1 to 10 of 10 for “"microscale thermophoresis"”.
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Development of PET Radiotracers for Imaging Neurodegeneration: Targeting alpha-Synuclein Fibrils and TSPO
… development of a novel biophysical technique – microscale thermophoresis (MST). A general automated radiosynthetic method to afford the [18F]fluoro-derivatives of these compounds has also been developed, and preliminary in vitro autoradiography studies and an in vivo microPET scan has been …
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Analysis of the biophysical and in silico exploration of the interaction between small ribonucleoprotein G and the RING finger domain of retinoblastoma binding protein 6 for the identification of protein anti-cancer compounds
… Pro101 and Met194. Biophysical studies using MicroScale Thermophoresis (MST) confirmed the putative SNRPG~RING Finger domain interaction, and determined that the binding affinity was a Kdvalue of 3.1596 nM under aqueous buffer conditions. The overall results from this study suggest the …
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Characterizing and Overcoming Resistance to Aminomethylspectinomycins in Gram-negative Bacteria
… studied using Thermal Denaturation assay and MicroScale Thermophoresis (MST).</p> <p>The microbiological role of these enzymes has been examined by minimum inhibitory concentration (MIC) shifts using an arabinose inducible expression of APH (9)-Ia and ANT (3")(9) in E.coli K12 and JW ΔtolC …
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Explorer l'impact de l'élimination de la charge nette cationique des signaux de localisation nucléaire (NLS) sur le ciblage par l'importine-α
… complexed to importin-ɑ. Circular dichroism and microscale thermophoresis were employed to evaluate NLS-importin-ɑ complex stabilities and binding affinities. Our results showed that zero net-charge NLSs could be developed with binding affinities and interaction modes comparable to those of …
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DESIGN AND DEVELOPMENT OF RNA-BASED DRUGS USING MOLECULAR DYNAMICS SIMULATIONS AND ARTIFICIAL INTELLIGENCE METHODS
… such as PRMT1 and limiting cancer cell survival. Microscale thermophoresis (MST) experiments on the PNA ligand AATAAA revealed a dissociation constant in the low nanomolar range. For HMGB1, sequence optimization of the initial ATAG motif led to a twofold improvement in predicted binding affinity. …
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DEVELOPMENT OF MOLECULAR PROBES ACTING ON aSYN/TUBULIN AND TAU/TUBULIN INTERACTION
… activities during the in vitro experiments. Microscale Thermophoresis (MST) and the tubulin polymerization assay were employed using these peptides, in conditions which were approximate to the physiological ones. From preliminary data, two mutants, seemed to affect the polymerization of …
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METFORMIN, CLIC1 AND TRANSCRANIAL STIMULATION: THE BASIS OF A NEW TARGETED THERAPY AGAINST GLIOBLASTOMA RELAPSE
… the direct metformin-CLIC1 interaction, by NMR, MicroScale Thermophoresis, and single-channel patch-clamp experiments. However, due to the blood brain barrier, the high concentration of metformin (10mM) required to inhibit tumor progression, which has been beneficial in other types of tumors, …
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Investigating the role of ICL3 in β1-adrenergic receptor activation
… Kd for Nb6B9, miniG and SH3 domain using microscale thermophoresis was obtained with all binding partners showing nanomolar affinities. The cAMP response in presence of a range of ligands was recorded using cellular assay comparing the chimera β1ARhICL3 with tβ1AR. In conclusion, the …
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Aptamer-Based Detection of Salivary Melatonin for Circadian Rhythm Sleep Disorders Monitoring
… using circular dichroism spectroscopy and microscale thermophoresis respectively. Different versions of the untagged aptamer i.e., full, and truncated sequence structure were studied under varying buffer and temperature conditions, while Cy5 labeled aptamers were used to determine the …