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Showing 1 to 13 of 13 for “"micropipette aspiration"”.
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Development of a novel micropipette aspiration-based force sensing technique to measure single cell sliding friction
… Thus, we propose an instrument based on the micropipette aspiration technique to solve this problem. In this setup, a highly sensitive friction force sensor is made out of a long, thin, and hollow micropipette that deflects as a function of the tangential force experienced by a cell, …
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Harmonic Acoustics for Single Cell Manipulation
… atomic force microscopy, optical tweezers, and micropipette aspiration, suffer from low throughputs, insufficient repeatability over the single cell assay, and physical contacts that can negatively influence testing results. On the other hand, conventional acoustic tweezers can manipulate cells …
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Multilevel, subdivision-based, thin shell finite elements: development and an application to red blood cell modeling
… constant-volume deformation of a red blood cell: micropipette aspiration and point load application.
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Cellular responses to mechanical stresses applied via magnetic manipulators
… force microscopy, optical and magnetic tweezers, micropipette aspiration and other techniques have been used to explore and develop physical models of the cell. By using these techniques, the basic physical response of a cell to mechanical forces has been analyzed, yet the reported cell moduli in …
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Mechanochemical pattern formation in the cellular actomyosin cortex
… external geometrical deformations by combining micropipette aspiration with live fluorescence imaging. In Chapter 4, I describe the infrared spectroscopy setup built in the pursuit of uncovering the properties of fluorescent markers. Taken together, the work in this thesis outlines a …
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Engineering Dynamic Granular Composites for the Repair of Cartilage Tissue
… of HA microgels were explored via building a micropipette aspiration device and validated through nanoindentation to understand differences between microgel and bulk properties. Next, granular composites are introduced and the volume ratio of spheroids and microgels were tailored in silico and …
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Phase separation in giant vesicles
… the membrane tension. This was demonstrated by micropipette aspiration experiments on vesicles encapsulating two phases. The budding of one phase can significantly decrease the surface energy by decreasing the contact area between the coexisting phases. The elasticity of the membrane allows it …
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Effects of Linoleic Acid on Tether Formation between Monocytes and Endothelial Cells
… adhesion remain unclear. Using a combination of micropipette aspiration techniques and fluorescent microscopy, I tested the hypothesis that linoleic acid increases membrane tether formation between monocytes and endothelial cells. </p><p>Treatment of U937 monocytes with free linoleic acid or …
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Study of T cell activation and migration at the single-cell and single-molecule level
… This hypothesis was tested using the techniques micropipette aspiration and atomic force microscopy, which allow the investigation of the elastic and viscous responses of single T cells. It was discovered that the activation process reduced T cell stiffness by more than three folds, a finding …
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Investigation of minimum fragment requirement for mimicking n-cadherin mediated adhesion
… wild type cadherins expressed on cell surfaces, Micropipette Aspiration Assay (MPA) was performed. Using N-cadherin as a model for classical cadherins, the binding affinity of full length N-cadherin (N-cad EC1-5) expressed on a test cell surface (here, mesenchymal stem cell (MSCs) with the first …
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Biomechanical and Molecular Approaches to Aortic Valve Disease in a Mouse Model
<p>Aortic valve disease (AVD) occurs in 2.5% of the human population and is a significant cause of cardiovascular mortality. Aortic valve malformation is a heritable condition that underlies the majority of cases, suggesting a developmental origin. Increased interstitial cell activation and …
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Characterization of the nano- and meso-scale hierarchic assembly in biomimetic membranes and living systems
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2026-12-01
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Multilamellar lipid structures and their elastic properties in living systems
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-08-01